Automated High-performance Liquid Chromatography Detection of Serum Retinol Levels in Cervical Cancer Patients Compared with Normal Subjects

2006 ◽  
Vol 18 (7) ◽  
pp. 577
Author(s):  
J. Abu ◽  
M. Batuwangala ◽  
P. Symonds ◽  
K. Herbert
Keyword(s):  
Cervical Cancer ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Cancer Patients ◽  
High Performance ◽  
Normal Subjects ◽  
Serum Retinol

Neutrophil-activating peptide-2 in patients with pulmonary edema from congestive heart failure or ARDS

1993 ◽  
Vol 264 (5) ◽  
pp. L490-L495 ◽  
Author(s):  
A. B. Cohen ◽  
M. D. Stevens ◽  
E. J. Miller ◽  
M. A. Atkinson ◽  
G. Mullenbach ◽  
...  
Keyword(s):  
Heart Failure ◽  
Congestive Heart Failure ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Pulmonary Edema ◽  
High Performance ◽  
Distress Syndrome ◽  
Normal Subjects ◽  
Antigen Concentration ◽  
Fluid Formation

We carried out studies to determine whether the neutrophil-activation peptide-2 (NAP-2) plays a role in the recruitment and/or degranulation of neutrophils into the lungs of patients with the adult respiratory distress syndrome (ARDS) or congestive heart failure (CHF). NAP-2 precursors plus NAP-2 (beta-thromboglobulin-like antigen) were measured in lung fluids and plasmas with a radioimmunoassay, and NAP-2 was separated from its precursors by high-performance liquid chromatography. Pulmonary edema fluids (PEFs) from patients with CHF contained higher concentrations of the beta-thromboglobulin-like antigen than PEFs from patients with ARDS, and bronchoalveolar lavage fluids (BALs) from patients with ARDS contained higher concentrations of beta-thromboglobulin-like antigen than BALs from normal subjects. beta-Thromboglobulin-like antigen concentration was 4.1-fold greater in PEFs from patients with CHF than in their plasmas. Chemotactically active NAP-2 was also demonstrated in PEFs but not in plasmas from patients with CHF and ARDS. These data suggest that significant platelet degranulation occurred into the lungs of the patients with CHF and that NAP-2 and other platelet constituents may contribute to fluid formation in patients with CHF.

Determination of Doxorubicin and Doxorubicinol in Plasma of Cancer Patients by High-Performance Liquid Chromatography

1999 ◽  
Vol 266 (2) ◽  
pp. 216-221 ◽  
Author(s):  
Peter de Bruijn ◽  
Jaap Verweij ◽  
Walter J. Loos ◽  
Herman J. Kolker ◽  
André S.T. Planting ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Cancer Patients ◽  
High Performance

Liquid-chromatographic profiling of endogenous fluorescent substances in sera and urine of uremic and normal subjects.

1983 ◽  
Vol 29 (4) ◽  
pp. 675-677 ◽  
Author(s):  
H Mabuchi ◽  
H Nakahashi
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Normal Subjects ◽  
Body Fluids ◽  
Normal Serum ◽  
Fluorescence Excitation ◽  
Normal Urine ◽  
Liquid Chromatographic ◽  
Chromatographic Profiling

Abstract Endogenous fluorescent substances increase in serum during uremia. We have used "high-performance" liquid chromatography to profile these fluorescent substances in both uremic and normal body fluids. The fluorescence excitation and emission maxima we used were 322 and 415 nm, respectively. Of the numerous fluorescent substances found in uremic body fluids and in normal urine, some were also detectable in normal serum, but at relatively weak fluorescence intensities.

scholarly journals Cytotoxic and Immunomodulatory Potential Activity of Physalis peruviana Fruit Extracts on Cervical Cancer (HeLa) and Fibroblast (L929) Cells

2017 ◽  
Vol 22 (4) ◽  
pp. 777-787 ◽  
Author(s):  
Helen Mier-Giraldo ◽  
Luis Eduardo Díaz-Barrera ◽  
Lucy Gabriela Delgado-Murcia ◽  
Manuel Fernando Valero-Valdivieso ◽  
Gabriela Cáez-Ramírez
Keyword(s):  
Cervical Cancer ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Interleukin 6 ◽  
High Performance ◽  
Interleukin 8 ◽  
Dependent Manner ◽  
L929 Cells ◽  
Physalis Peruviana ◽  
Immunomodulatory Potential

It was purposed to evaluate the biological potential of ethanol and isopropanol crude extracts of ripe Physalis peruviana fruits. Cytotoxic and immunomodulatory effects of the expression of interleukin-6, interleukin-8, and monocyte chemoattractant protein-1 (MCP-1) were evaluated on human cervical cancer (HeLa) and murine fibroblast (L929) cells. The composition was evaluated by high-performance liquid chromatography diode-array detection and high-performance liquid chromatography ultraviolet/visible detection. The presence of ursolic acid and rosmarinic acid was found in both solvents. However, gallic acid, quercetin, and epicatechin were higher in isopropanol extracts ( P < .05). The results indicated a relationship among the total polyphenol content, antioxidant activity, and cytotoxic activity that was dependent on the solvent used. Isopropanol extracts presented a half-maximal inhibition concentration value (IC50) of 60.48 ± 3.8 μg/mL for HeLa cells and 66.62 ± 2.67 μg/mL for L929 fibroblasts. The extracts reduced the release of interleukin-6, interleukin-8, and MCP-1 in a dose-dependent manner. Extracts showed anticancer and immunomodulatory potential for new complementary pharmaceutical products development.

Characterization by high-performance liquid chromatography of circulating growth hormone-releasing factors in normal subjects

1986 ◽  
Vol 111 (3) ◽  
pp. 507-511 ◽  
Author(s):  
E. S. Penny ◽  
A. M. Sopwith ◽  
R. L. Patience ◽  
J. A. H. Wass ◽  
G. M. Besser ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Large Scale ◽  
Molecular Form ◽  
Normal Subjects ◽  
Reversed Phase ◽  
Percentage Increase ◽  
Mixed Meal ◽  
Analytical Separation

ABSTRACT Four forms of circulating immunoreactive human GH-releasing factor (ir-hGRF) have been identified in each of four normal subjects, with a mean increase in total ir-hGRF of twofold over basal levels following a mixed meal. Plasma samples (200 ml) from each individual were subjected to large-scale Vycor extraction with initial purification by high-performance liquid chromatography on a reversed-phase C18 column, followed by analytical separation of the ir-hGRF components using a C3 wide-pore reversed-phase column, and subsequent radioimmunoassay of the fractions. The mean recovery of total ir-hGRF from the plasma (fasted and non-fasted) was 76±16% (2×s.e.m.). Analytical separation of the ir-hGRF revealed four components which co-eluted with synthetic hGRF-37, hGRF-40 and hGRF-44, and a peak eluting between hGRF-40 and -44 which may represent hGRF-42. The hGRF-40 was shown to be the predominant circulating molecular form in the fasted state in each subject, and in three out of four subjects following a mixed meal. The hGRF-44 showed the greatest percentage increase over basal in all four individuals. J. Endocr. (1986) 111, 507–511

Screening breast cancer patients forATM mutations and polymorphisms by using denaturing high-performance liquid chromatography

2001 ◽  
Vol 38 (2-3) ◽  
pp. 200-208 ◽  
Author(s):  
David P. Atencio ◽  
Christopher M. Iannuzzi ◽  
Sheryl Green ◽  
Richard G. Stock ◽  
Jonine L. Bernstein ◽  
...  
Keyword(s):  
Breast Cancer ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Cancer Patients ◽  
High Performance ◽  
Breast Cancer Patients

Effect of Morphine and Bupivacaine on Nicotine-Induced Catecholamine Secretion from Encapsulated Chromaffin Cells

2005 ◽  
Vol 277-279 ◽  
pp. 56-61
Author(s):  
Hai Yan Hong ◽  
Jeong Ok Lim ◽  
Woon Yi Baek
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Cancer Patients ◽  
Chromaffin Cells ◽  
High Performance ◽  
Hplc Analysis ◽  
Intractable Pain ◽  
Analgesic Effects ◽  
Bovine Chromaffin Cells ◽  
Analysis System

The control of intractable pain through transplanted of chromaffin cells has been recently reported where the analgesic effects are principally due to the production of opioid peptides and catecholamines (CAs) by the chromaffin cells. Currently many cancer patients receive general opioids or local anesthetics, such as bupivacaine. Therefore, the present study investigated the effect of morphine or bupivacaine on the secretion of nicotine-induced CAs from encapsulated chromaffin cells over a period of 180 min. As such, bovine chromaffin cells were isolated and encapsulated with alginate–poly–L–lysine–alginate (APA) biomaterials to prevent immunorejection. The capsules were then pre-incubated with nicotine for 5 min prior to morphine or bupivacaine stimulation, and the quantity of CAs analyzed using a high performance liquid chromatography (HPLC) analysis system. The resulting data showed that the encapsulated chromaffin cells retained the ability of their parent chromaffin cells when responding to opioids by suppressing the release of CAs. In contrast, bupivacaine did not have any statistically significant affect on the basal and nicotine-induced CA release from the encapsulated chromaffin cells.

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