scholarly journals Study on vitamin A reference standard for high performance liquid chromatography. Formulation of retinyl palmitate soft capsule and evaluation of its quality and stability.

1986 ◽  
Vol 35 (3) ◽  
pp. T12-T15
Author(s):  
Takao INOUE ◽  
Yukio SHOJI ◽  
Yasuji TAKASHIMA ◽  
Reijiro KOHNO ◽  
Hitoshi EZAWA ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Vitamin A ◽  
High Performance ◽  
Retinyl Palmitate ◽  
Reference Standard

Quantification of Vitamin A in Fortified Rapeseed, Groundnut and Soya Oils Using a Simple Portable Device: Comparison to High Performance Liquid Chromatography

2013 ◽  
Vol 83 (2) ◽  
pp. 122-128 ◽  
Author(s):  
Cécile Renaud ◽  
Jacques Berger ◽  
Arnaud Laillou ◽  
Sylvie Avallone
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Vitamin A ◽  
High Performance ◽  
Vitamin A Deficiency ◽  
Developed Countries ◽  
Portable Device ◽  
Least Developed Countries ◽  
Control Food ◽  
Assay Precision

Vitamin A deficiency is still one of the major public health problems in least developed countries. Fortification of vegetable oils is a strategy implemented worldwide to prevent this deficiency. For a fortification program to be effective, regular monitoring is necessary to control food quality in the producing units. The reference methods for vitamin A quantification are expensive and time-consuming. A rapid method should be useful for regular assessment of vitamin A in the oil industry. A portable device was compared to high-performance liquid chromatography (HPLC) for three plant oils (rapeseed, groundnut, and soya). The device presented a good linearity from 3 to 30 mg retinol equivalents per kg (mg RE.kg- 1). Its limits of detection and quantification were 3 mg RE.kg- 1 for groundnut and rapeseed oils and 4 mg RE.kg- 1 for soya oil. The intra-assay precision ranged from 1.48 % to 3.98 %, considered satisfactory. Accuracy estimated by the root mean squares error ranged from 3.99 to 5.49 and revealed a lower precision than HPLC (0.4 to 2.25). Although it offers less precision than HPLC, the device estimates quickly the vitamin A content of the tested oils from 3 or 4 to 15 mg RE.kg- 1.

Tingginya tingkat konsumsi minyak goreng oleh Masyarakat Indonesia menjadikan minyak goreng digunakan sebagai food vehiclepada program fortifikasi vitamin A.Program tersebut dilakukan untuk mengatasi Kekurangan vitamin A (KVA). KVAsangat mempengaruhi kela

2018 ◽  
Vol 5 (1) ◽  
Author(s):  
LILY RESTUSARI ◽  
SRI WIDIA NINGSIH ◽  
YULIANA ARSIL
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Vitamin A ◽  
High Performance

Tingginya tingkat konsumsi minyak goreng oleh Masyarakat Indonesia menjadikan minyak goreng digunakan sebagai food vehiclepada program fortifikasi vitamin A.Program tersebut dilakukan untuk mengatasi Kekurangan vitamin A (KVA). KVAsangat mempengaruhi kelangsungan hidup anak dan standar upaya penyelamatan proses kehamilan dan persalinan. Namun pemanasan yang terjadi selama proses penggorenganpada minyak dapatmerusak jumlah vitamin A yang difortifikasi. Tujuan penelitian ini untuk membandingkan nilai retensi vitamin A di dalam kentang yang digoreng dengan menggunakan minyak goreng curah dan minyak goreng kemasan fortifikasi dengan pengaruh suhu dan waktu penggorengan. Penelitian ini menggunakan metode eksperimental disain rancangan acak lengkap (RAL) dengan dua faktor, suhu (140dan 170oC) dan waktu penggorengan (10 dan 15 menit) tanpa pengulangan.Sampel penelitian yang digunakan adalah kentang beku, minyak goreng curah dan minyak goreng kemasan fortifikasi.Nilai retensi vitamin A kentang dan minyak goreng diukur dengan menggunakan metode High Performance Liquid Chromatography (HPLC).Analisis perbedaan dan interaksi variabel diolah dengan ANOVA dua arah. Berdasarkan luas area puncak vitamin A pada kromatogram sebelum dan setelah penggorengan, ditemukan kecenderungan penurunan persentase retensi vitamin A dari minyak goreng kemasan fortifikasi dibandingkan minyak goreng curah.Nilai retensi vitamin A pada kentang yang digoreng dengan minyak goreng curah adalah 217-251%,sedangkan retensivitamin A kentang yang digoreng dengan minyak goreng kemasan fortifikasi adalah 48-131%. Analisis ANOVA dua arah tanpa pengulangan untuk persen retensi vitamin A menunjukkan bahwa (p<0,05) variabel jenis minyak goreng berpengaruh secara nyata terhadap retensi vitamin A. Retensi vitamin A pada kentang goreng yang digoreng dengan minyak goreng curah jauh lebih tinggi dibanding minyak goreng kemasan fortifikasi

scholarly journals Authentication of the Bilberry Extracts by an HPLC Fingerprint Method Combining Reference Standard Extracts

Molecules ◽  
2020 ◽  
Vol 25 (11) ◽  
pp. 2514
Author(s):  
Bingbing Liu ◽  
Tiantian Hu ◽  
Weidong Yan
Keyword(s):  
Quality Control ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Reference Standard ◽  
Black Rice ◽  
Hplc Fingerprint ◽  
Berry Extracts ◽  
Bilberry Extract

A simple and fast high-performance liquid chromatography (HPLC) fingerprint method combining reference standard extract for the identification of bilberry extract was developed and validated. Six batches of bilberry extract collected from different manufactures were used to establish the HPLC fingerprint. Other berry extracts—such as blueberry extracts, mulberry extracts, cranberry extracts, and black rice extracts—were also analyzed for their HPLC chromatograms. The fingerprints of five batches of bilberry extract showed high similarities, while one batch was distinguished from others. Additionally, the content of anthocyanin Cyanidin-3-O-glucoside (Cy-3-glc) in each berry extract was analyzed and compared. The results indicate that this HPLC fingerprint method, combining reference standard extracts, could be used for the authentication and quality control of bilberry extracts.

scholarly journals Simultaneous determination of retinol, retinyl palmitate and β-carotene in rat serum treated with 7,12-dimethylbenz[a]anthracene and Hypericum Perforatum L. by high-performance liquid chromatography with diode-array detection

2010 ◽  
Vol 8 (1) ◽  
pp. 108-115 ◽  
Author(s):  
Abdulkadir Levent ◽  
Suat Ekin ◽  
Gökhan Oto
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Hypericum Perforatum ◽  
Treated Group ◽  
Retinyl Palmitate ◽  
Rat Serum ◽  
Array Detection ◽  
Hypericum Perforatum L ◽  
Β Carotene

AbstractA new and simple high-performance liquid chromatography method was developed and validated for the simultaneous determination of retinol, retinyl palmitate and β-carotene in rat serum treated with Hypericum Perforatum L. and 7,12-dimethylbenz[a]anthracene. Furthermore, vitamin C was determined spectrophotometrically. High-performance liquid chromatography analysis was performed utilizing an Inertsil ODS3 reversed phase column with methanol-acetonitrile-tetrahydrofuran (65:30:5, v/v/v) as mobile phase, at a flow rate of 1.5 mL min−1 and 40°C. Diode-array detection was conducted at 325 and 450 nm for retinol and retinyl palmitate, and β-carotene, respectively with a running time of 26 min. The high-performance liquid chromatography assay and extraction procedure proposed are simple, rapid, sensitive and accurate. This method was then applied to determine the amounts of retinol, retinyl palmitate and β-carotene in rat serum. Results of this study demonstrated that at 60th day in the 7,12-dimethylbenz[a]anthracene-treated group there was a significant decrease (pa] anthracene + Hypericum Perforatum L. treated group compared to the control group..

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