scholarly journals Enhanced secretion of tropane alkaloids in Nicotiana tabacum hairy roots expressing heterologous hyoscyamine-6β-hydroxylase

2005 ◽  
Vol 56 (420) ◽  
pp. 2611-2618 ◽  
Author(s):  
Suvi T. Häkkinen ◽  
Elisabeth Moyano ◽  
Rosa M. Cusidó ◽  
Javier Palazón ◽  
M. Teresa Piñol ◽  
...  
Keyword(s):  
Nicotiana Tabacum ◽  
Hairy Roots ◽  
Tropane Alkaloids ◽  
Enhanced Secretion

Antioxidant response of tobacco (Nicotiana tabacum) hairy roots after phenol treatment

2011 ◽  
Vol 49 (9) ◽  
pp. 1020-1028 ◽  
Author(s):  
Lucas G. Sosa Alderete ◽  
Elizabeth Agostini ◽  
María I. Medina
Keyword(s):  
Nicotiana Tabacum ◽  
Hairy Roots ◽  
Antioxidant Response ◽  
Phenol Treatment

scholarly journals Production of tropane alkaloids in Hyoscyamus niger (black henbane) hairy roots grown in bubble-column and spray bioreactors

2013 ◽  
Vol 36 (4) ◽  
pp. 843-853 ◽  
Author(s):  
Zbigniew Jaremicz ◽  
Maria Luczkiewicz ◽  
Adam Kokotkiewicz ◽  
Aleksandra Krolicka ◽  
Pawel Sowinski
Keyword(s):  
Hairy Roots ◽  
Bubble Column ◽  
Tropane Alkaloids ◽  
Hyoscyamus Niger ◽  
Black Henbane

scholarly journals Expression of the antibody 14D9 in Nicotiana tabacum hairy roots

2005 ◽  
Vol 8 (2) ◽  
pp. 170-176 ◽  
Author(s):  
Carolina Martinez ◽  
Silvana Petruccelli ◽  
Ana Maria Giulietti ◽  
Maria Alejandra Alvarez
Keyword(s):  
Nicotiana Tabacum ◽  
Hairy Roots

CRISPR/Cas9-mediated disruption of the PYRROLIDINE KETIDE SYNTHASE gene reduces the accumulation of tropane alkaloids in Atropa belladonna hairy roots

2021 ◽  
Author(s):  
Fumihito Hasebe ◽  
Honoka Yuba ◽  
Takashi Hashimoto ◽  
Kazuki Saito ◽  
Nobutaka Funa ◽  
...  
Keyword(s):  
Hairy Roots ◽  
Biosynthetic Pathway ◽  
Polyketide Synthase ◽  
Tropane Alkaloids ◽  
Tropane Alkaloid ◽  
Atropa Belladonna ◽  
Type Iii Polyketide Synthase ◽  
Canonical Type ◽  
Solanaceae Family

Abstract Tropane alkaloids, including clinically important hyoscyamine and scopolamine, are produced in the roots of medicinal plant species, such as Atropa belladonna, from the Solanaceae family. Recent molecular and genomic approaches have advanced our understanding of the metabolic enzymes involved in tropane alkaloid biosynthesis. A non-canonical type III polyketide synthase (PKS), pyrrolidine ketide synthase (PYKS), catalyzes a two-step decarboxylative reaction, which involves imine-ketide condensation indispensable to tropane skeleton construction. In this study, we generated pyks mutant A. belladonna hairy roots via CRISPR/Cas9-mediated genome editing and analyzed the metabolic consequences of the loss of PYKS activity on tropane alkaloids, providing insights into a crucial role of the scaffold-forming reaction in the biosynthetic pathway.

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