Induced production of tropane alkaloids, and expression of hyoscyamine 6β-hydroxylase (h6h) and putrescine N-methyl transferase (pmt2) genes in hairy roots and propagated plantlets of Atropa belladonna L. elicited by methyl jasmonate

2020 ◽  
Vol 131 ◽  
pp. 328-334 ◽  
Author(s):  
Azar Moradi ◽  
Mohsen Sharifi ◽  
Amir Mousavi
Keyword(s):  
Methyl Jasmonate ◽  
Hairy Roots ◽  
Tropane Alkaloids ◽  
Atropa Belladonna ◽  
Methyl Transferase

CRISPR/Cas9-mediated disruption of the PYRROLIDINE KETIDE SYNTHASE gene reduces the accumulation of tropane alkaloids in Atropa belladonna hairy roots

2021 ◽  
Author(s):  
Fumihito Hasebe ◽  
Honoka Yuba ◽  
Takashi Hashimoto ◽  
Kazuki Saito ◽  
Nobutaka Funa ◽  
...  
Keyword(s):  
Hairy Roots ◽  
Biosynthetic Pathway ◽  
Polyketide Synthase ◽  
Tropane Alkaloids ◽  
Tropane Alkaloid ◽  
Atropa Belladonna ◽  
Type Iii Polyketide Synthase ◽  
Canonical Type ◽  
Solanaceae Family

Abstract Tropane alkaloids, including clinically important hyoscyamine and scopolamine, are produced in the roots of medicinal plant species, such as Atropa belladonna, from the Solanaceae family. Recent molecular and genomic approaches have advanced our understanding of the metabolic enzymes involved in tropane alkaloid biosynthesis. A non-canonical type III polyketide synthase (PKS), pyrrolidine ketide synthase (PYKS), catalyzes a two-step decarboxylative reaction, which involves imine-ketide condensation indispensable to tropane skeleton construction. In this study, we generated pyks mutant A. belladonna hairy roots via CRISPR/Cas9-mediated genome editing and analyzed the metabolic consequences of the loss of PYKS activity on tropane alkaloids, providing insights into a crucial role of the scaffold-forming reaction in the biosynthetic pathway.

Differential Production of Tropane Alkaloids in Hairy Roots and in vitro Cultured Two Accessions of Atropa belladonna L. under Nitrate Treatments

2010 ◽  
Vol 65 (5-6) ◽  
pp. 373-379 ◽  
Author(s):  
Najmeh Ahmadian Chashmi ◽  
Mozafar Sharifi ◽  
Farah Karimi ◽  
Hasan Rahnama
Keyword(s):  
Secondary Metabolites ◽  
Hairy Roots ◽  
Nitrate Concentration ◽  
Tropane Alkaloids ◽  
Atropa Belladonna ◽  
Remarkable Effect ◽  
Aerial Parts ◽  
Potential Source ◽  
Stable Production

Plants are a potential source of a large number of valuable secondary metabolites. In vitro cultures are being considered as an alternative to agricultural processes for studying valuable secondary metabolites. In this way, nutritive factors are important parameters influencing the production of these compounds in plants. Effects of nitrate concentrations (KNO3) on the production of two tropane alkaloids, hyoscyamine and scopolamine, and the growth of aerial parts and roots of two in vitro propagated accessions of Atropa belladonna and hairy roots were investigated. As hairy roots cultures are able to keep a stable production of alkaloids over long periods of subculturing, they are considered as an interesting option for the study of alkaloid biosynthesis. A hairy roots culture of Atropa belladonna was established by transformation with Agrobacterium rhizogenes strain AR15834. The results of our study showed that a rise in KNO3 concentration caused a decline in hairy roots growth, and had a remarkable effect on the alkaloid content. The alkaloid concentrations obtained in the hairy roots were 3 - 20 times higher than that in the plants at 35 mM of KNO3. Increasing the nitrate concentration in the medium of hairy roots also improved the hyoscyamine/scopolamine ratio, while it increased the scopolamine/hyoscyamine ratio in the studied plants.

scholarly journals Erratum: Production of Sesquiterpene-Type Phytoalexins by Hairy Roots of Hyoscyamus albus Co-treated with Cupper Sulfate and Methyl Jasmonate [Chem. Pharm. Bull. 58(7): 934-938 (2010)]

2010 ◽  
Vol 58 (9) ◽  
pp. 1281
Author(s):  
Moriyuki Kawauchi ◽  
Toshihide Arima ◽  
Osamu Shirota ◽  
Setsuko Sekita ◽  
Takahisa Nakane ◽  
...  
Keyword(s):  
Methyl Jasmonate ◽  
Hairy Roots ◽  
Hyoscyamus Albus

Investigation of Tropane Alkaloids in Genetically Transformed Atropa belladonna L. Cultures

2004 ◽  
Author(s):  
H. Hank ◽  
�. Sz?ke ◽  
K. T�th ◽  
I. L�szl� ◽  
L. Kursinszki
Keyword(s):  
Tropane Alkaloids ◽  
Atropa Belladonna

Influence of Methyl Jasmonate on Production of Ecdysteroids from Hairy Roots of Silene linicola C.C. Gmelin

2019 ◽  
Vol 45 (7) ◽  
pp. 920-926 ◽  
Author(s):  
A. A. Erst ◽  
L. N. Zibareva ◽  
E. S. Filonenko ◽  
T. V. Zheleznichenko
Keyword(s):  
Methyl Jasmonate ◽  
Hairy Roots

scholarly journals Enhanced production of valtrate in hairy root cultures of Valeriana jatamansi Jones by methyl jasmonate, jasmonic acid and salicylic acid elicitors

2020 ◽  
Vol 48 (2) ◽  
pp. 839-848
Author(s):  
Shuang ZHAO ◽  
Hong TANG
Keyword(s):  
Salicylic Acid ◽  
Jasmonic Acid ◽  
Methyl Jasmonate ◽  
Hairy Root ◽  
Hairy Roots ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Potent Inducer ◽  
Valeriana Jatamansi ◽  
Pharmacologically Active

Valtrate is a pharmacologically active epoxyiridoid ester found in the roots and rhizomes of Valeriana jatamansi Jones. The plant produces only small amounts of this metabolite naturally, and so induction of hairy roots as well as elicitation can be useful to increase its commercial production. In this study, strain R1601 of Agrobacterium rhizogenes was used to induce hairy roots in V. jatamansi, and stable hairy root cultures of V. jatamansi were established successfully. The influence of three exogenous elicitors including methyl jasmonate (MJ), jasmonic acid (JA) and salicylic acid (SA) on valtrate production in the hairy root cultures of V. jatamansi was also investigated, and the 25-day-old hairy root cultures were treated with different concentrations of the elicitors at exposure time of 7 days. This present study showed that MJ (100 mg/L) highly promoted valtrate production at 7 days after elicitation, to a level of 3.63 times higher than that of non-elicited control. SA did not significantly increase the production of valtrate. This is the first-time study to assess the elicitation of hairy root cultures to promote valtrate biosynthesis in V. jatamansi and the resulting experiments demonstrated that MJ was indeed a potent inducer of valtrate biosynthesis.

scholarly journals Enhanced secretion of tropane alkaloids in Nicotiana tabacum hairy roots expressing heterologous hyoscyamine-6β-hydroxylase

2005 ◽  
Vol 56 (420) ◽  
pp. 2611-2618 ◽  
Author(s):  
Suvi T. Häkkinen ◽  
Elisabeth Moyano ◽  
Rosa M. Cusidó ◽  
Javier Palazón ◽  
M. Teresa Piñol ◽  
...  
Keyword(s):  
Nicotiana Tabacum ◽  
Hairy Roots ◽  
Tropane Alkaloids ◽  
Enhanced Secretion
Sign in / Sign up

Export Citation Format

Share Document