Basic principles and technological state of the art: SPECT

Basic Principles ◽

Non Invasive ◽

Single-photon emission computed tomography (SPECT) photons as a medical imaging technique detects the radiation emitted by radioisotopes injected into the body to provide in vivo measurements of regional tissue function. From its introduction in the cardiologic clinical field, nuclear imaging has classically represented the reference technique for the non-invasive evaluation of myocardial perfusion, becoming the most frequently performed imaging modality for the functional assessment of patients with ischaemic heart disease.

Radioactive Labeling of Milk-Derived Exosomes with 99mTc and In Vivo Tracking by SPECT Imaging

Nanomaterials ◽

10.3390/nano10061062 ◽

2020 ◽

Vol 10 (6) ◽

pp. 1062 ◽

Cited By ~ 4

Author(s):

María Isabel González ◽

Pilar Martín-Duque ◽

Manuel Desco ◽

Beatriz Salinas

Keyword(s):

Single Photon ◽

Photon Emission ◽

Spect Imaging ◽

Histological Techniques ◽

Non Invasive ◽

Longitudinal Tracking ◽

Pharmacokinetic Properties ◽

Over the last decade, exosomes from diverse biological sources have been proposed as new natural platforms in drug delivery. Translation of these nanometric tools to clinical practice requires deep knowledge of their pharmacokinetic properties and biodistribution. The pharmacokinetic properties of exosomes are sometimes evaluated using biochemical and histological techniques that are considerably invasive. As an alternative, we present radiochemical labeling of milk-derived exosomes based on reduced 99mTc (IV) without modifying biological and physicochemical properties. This approach enables longitudinal tracking of natural exosomes by non-invasive single photon emission computed tomography (SPECT) imaging and the evaluation of their pharmacokinetic properties according to the route of administration.

In vivo functional imaging with SPECT and PET

Radiochimica Acta ◽

10.1524/ract.2001.89.4-5.203 ◽

2001 ◽

Vol 89 (4-5) ◽

Cited By ~ 2

Author(s):

H. Herzog

Keyword(s):

Spatial Information ◽

Single Photon ◽

Photon Emission ◽

The Body ◽

Physiological Processes ◽

Positron Emission ◽

In Vivo Functional Imaging ◽

Nuclear medicine methods permit the visualisation of a variety of metabolic and physiological processes all over the body. Although planar scintigraphy has been found useful for many questions, detailed spatial information about the diseased organ can only be obtained with tomographic methods. Dependent on the radionuclide involved, two different tomographic procedures are available: single photon emission computed tomography (SPECT) and positron emission tomography (PET). The first part of this paper describes shortly the historical development of these methods as well as their technical and methodological basics. To elucidate the large variety of possible applications, an overview of SPECT and PET procedures utilised in research as well as in clinical practice are presented. Furthermore, both methods are compared and their individual advantages are pointed out.

Non-invasive evaluation of myocardial reperfusion by transthoracic Doppler echocardiography and single-photon emission computed tomography in patients with anterior acute myocardial infarction

Cardiovascular Ultrasound ◽

10.1186/1476-7120-9-16 ◽

2011 ◽

Vol 9 (1) ◽

Cited By ~ 3

Author(s):

Egle Sadauskiene ◽

Diana Zakarkaite ◽

Ligita Ryliskyte ◽

Jelena Celutkiene ◽

Alfredas Rudys ◽

...

Keyword(s):

Single Photon ◽

Photon Emission ◽

Myocardial Reperfusion ◽

Single Photon Emission ◽

Transthoracic Doppler Echocardiography ◽

Non Invasive ◽

Single Photon Emission Computed ◽

Photon Emission Computed Tomography ◽

Invasive Evaluation

Magnetic Resonace–Based Attenuation Correction for Micro–Single-Photon Emission Computed Tomography

Molecular Imaging ◽

10.2310/7290.2011.00036 ◽

2012 ◽

Vol 11 (2) ◽

pp. 7290.2011.00036 ◽

Cited By ~ 4

Author(s):

Vincent Keereman ◽

Yves Fierens ◽

Christian Vanhove ◽

Tony Lahoutte ◽

Stefaan Vandenberghe

Keyword(s):

Attenuation Correction ◽

Single Photon ◽

Photon Emission ◽

The Body ◽

Micro Ct ◽

Mr Images ◽

Brain Scans ◽

The Brain ◽

Attenuation correction is necessary for quantification in micro–single-photon emission computed tomography (micro-SPECT). In general, this is done based on micro–computed tomographic (micro-CT) images. Derivation of the attenuation map from magnetic resonance (MR) images is difficult because bone and lung are invisible in conventional MR images and hence indistinguishable from air. An ultrashort echo time (UTE) sequence yields signal in bone and lungs. Micro-SPECT, micro-CT, and MR images of 18 rats were acquired. Different tracers were used: hexamethylpropyleneamine oxime (brain), dimercaptosuccinic acid (kidney), colloids (liver and spleen), and macroaggregated albumin (lung). The micro-SPECT images were reconstructed without attenuation correction, with micro-CT-based attenuation maps, and with three MR-based attenuation maps: uniform, non-UTE-MR based (air, soft tissue), and UTE-MR based (air, lung, soft tissue, bone). The average difference with the micro-CT-based reconstruction was calculated. The UTE-MR-based attenuation correction performed best, with average errors ≤ 8% in the brain scans and ≤ 3% in the body scans. It yields nonsignificant differences for the body scans. The uniform map yields errors of ≤ 6% in the body scans. No attenuation correction yields errors ≥ 15% in the brain scans and ≥ 25% in the body scans. Attenuation correction should always be performed for quantification. The feasibility of MR-based attenuation correction was shown. When accurate quantification is necessary, a UTE-MR-based attenuation correction should be used.