Sequence variation of the internal transcribed spacer (ITS) region of ribosomal DNA in Cerastoderma species (Bivalvia: Cardiidae)

Peucedanum huangshanense, a new species discovered in Anhui, China, is illustrated and described. Detailed morphological comparisons have showed that it is similar to P. praeruptorum but differs from the latter by having larger compound umbels (5–14 cm across), rays up to 25, and long-ovoid mericarps with lateral ribs narrowly winged. To explore the phylogenetic position of this species, nuclear ribosomal DNA internal transcribed spacer (ITS) region was sequenced for P. huangshanense and P. praeruptorum collected in different places. The morphological and molecular evidences support the hypothesis that P. huangshanense is a new distinct species.

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Detection of multiple infections by Monocystis strains in a single earthworm host using ribosomal internal transcribed spacer sequence variation

Parasitology ◽

10.1017/s0031182009990722 ◽

2009 ◽

Vol 137 (1) ◽

pp. 45-51 ◽

Cited By ~ 3

Author(s):

T. P. VELAVAN ◽

H. SCHULENBURG ◽

N. K. MICHIELS

Keyword(s):

Ribosomal Rna ◽

Internal Transcribed Spacer ◽

Sequence Variation ◽

Lumbricus Terrestris ◽

Its Region ◽

Seminal Vesicles ◽

Multiple Infections ◽

Apicomplexan Parasites ◽

Single Host ◽

Ribosomal Cistron

SUMMARYMonocystis sp. are sporocyst-forming apicomplexan parasites common in seminal vesicles of the earthworm Lumbricus terrestris where they may account for temporary castration. This study describes the internal transcribed spacer (ITS) region of the ribosomal cistron of Monocystis sp. This region, including ITS-1, the 5·8S ribosomal RNA gene, and ITS-2, was PCR amplified, cloned, and sequenced for Monocystis sp. isolated from the seminal vesicles of several wild-caught L. terrestris. Our analysis revealed substantial polymorphisms, also within single host organisms, indicating intra-host diversity of parasites. These genetic markers are the first that allow distinction of Monocystis sp. genotypes, opening new avenues for the study of parasite diversity within and between hosts.

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Assessment of genetic diversity among different sugarcane genotypes using internal transcribed spacer (ITS) region of the ribosomal DNA (rDNA)

GSC Biological and Pharmaceutical Sciences ◽

10.30574/gscbps.2018.5.2.0108 ◽

2018 ◽

Vol 5 (2) ◽

pp. 017-025

Author(s):

Farsangi Forough Jomhe ◽

Avinash Shivaji Thorat ◽

Rachayya Mallikarjun Devarumath

Keyword(s):

Genetic Diversity ◽

Ribosomal Dna ◽

Internal Transcribed Spacer ◽

Its Region

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DNAbased detection of western gall rust

New Zealand Plant Protection ◽

10.30843/nzpp.2004.57.6894 ◽

2004 ◽

Vol 57 ◽

pp. 146-150 ◽

Cited By ~ 1

Author(s):

T.D. Ramsfield ◽

D.R. Vogler

Keyword(s):

Ribosomal Dna ◽

Internal Transcribed Spacer ◽

Rust Fungus ◽

Its Region ◽

Pcr Primers ◽

Host Tissue ◽

Nuclear Ribosomal Dna ◽

Infected Host ◽

The Novel ◽

Pcr Product

The internal transcribed spacer (ITS) region of the nuclear ribosomal DNA of the western gall rust fungus (Peridermium harknessii) was amplified using the basidiomycetespecific PCR primers ITS1F and ITS4B The PCR product was then sequenced and aligned with other pine stem rust ITS sequences and a conserved region within P harknessii was targeted with the novel PCR primer Phar1 Our PCR protocol was able to differentiate P harknessii from Cronartium comandrae and C coleosporioides and detected P harknessii within infected host tissue However P harknessii was not distinguishable from C quercuum fsp fusiforme The method provides a rapid and sensitive detection protocol for P harknessii and C quercuum fsp fusiforme within infected host tissue

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Teleomorph Formation of Phaeoacremonium aleophilum, Cause of Esca and Grapevine Decline in California

Plant Disease ◽

10.1094/pd-89-0177 ◽

2005 ◽

Vol 89 (2) ◽

pp. 177-184 ◽

Cited By ~ 23

Author(s):

S. Rooney-Latham ◽

A. Eskalen ◽

W. D. Gubler

Keyword(s):

Vitis Vinifera ◽

Ribosomal Dna ◽

Internal Transcribed Spacer ◽

Type System ◽

Its Region ◽

Wood Chips ◽

Nuclear Ribosomal Dna ◽

Mating Types ◽

Molecular Analyses

Phaeoacremonium is a recently described genus of the hyphomycetes and includes species associated with grapevine (Vitis vinifera) declines worldwide. Spores of Phaeoacremonium spp. have been trapped in infested vineyards, but neither asexual nor sexual fruiting structures have been observed in the field. Mating studies were carried out to determine if California P. aleophilum isolates are capable of forming a teleomorph in vitro. Sterilized grapevine shavings were placed on the surface of water agar plates with pairs of different California isolates of P. aleophilum, an isolate from the holotype of P. aleophilum, plus other related Phaeoacremonium spp. After approximately 28 to 35 days, perithecia were seen forming on wood chips and agar of many pairings. Upon maturation, fertile perithecia had gelatinous droplets of ascospores oozing from their ostioles. Successful crosses, resulting in mature perithecia, corresponded to a heterothallic mating type system. When F1 progeny were backcrossed with their parents, heterothallism was confirmed. Molecular analyses of the internal transcribed spacer (ITS) region of the nuclear ribosomal DNA from ascospore progeny and perithecia confirmed that these perithecia were the teleomorph of P. aleophilum, Togninia minima. Furthermore, 4 months after moist incubating grapevine pieces from naturally infected vineyards, mature perithecia of T. minima could be seen forming on the xylem and pith tissues, suggesting both mating types occur on the same vine.

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A Survey of Phytophthora spp. in Midwest Nurseries, Greenhouses, and Landscapes

Plant Disease ◽

10.1094/pdis-07-12-0689-re ◽

2013 ◽

Vol 97 (5) ◽

pp. 635-640 ◽

Cited By ~ 17

Author(s):

A. J. Leonberger ◽

C. Speers ◽

G. Ruhl ◽

T. Creswell ◽

J. L. Beckerman

Keyword(s):

Host Range ◽

Ribosomal Dna ◽

Internal Transcribed Spacer ◽

Parental Species ◽

Its Region ◽

Cloning And Sequencing ◽

Hybrid Species ◽

Phytophthora Spp ◽

Two Hybrid

A survey of nurseries, greenhouses, and landscapes was conducted from 2006 to 2008 in order to determine the prevalence and diversity of Phytophthora spp. From sites in Iowa, Michigan, Ohio, and, predominantly, Indiana, 121 Phytophthora isolates were obtained from 1,657 host samples spanning 32 host genera. Based on sequence of the internal transcribed spacer (ITS) region of the ribosomal DNA, 11 Phytophthora spp. and two hybrid species were identified. A majority of the isolates were P. citricola (35.9%) or P. citrophthora (27.4%). Six isolates were confirmed as hybrids (four of P. cactorum × hedraiandra and two of P. nicotianae × cactorum) by cloning and sequencing the ITS region. Three P. cactorum × hedraiandra isolates were obtained from the same site, from three Rhododendron spp., which are known hosts to the parental species. The fourth isolate, however, was recovered out of a different location in a Dicentra sp., which is not a known host to either parental species, suggesting an expansion of host range of the hybrid isolate as compared with either parental species.

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Intragenomic sequence variations in the second internal transcribed spacer (ITS2) ribosomal DNA of the malaria vector Anopheles stephensi

PLoS ONE ◽

10.1371/journal.pone.0253173 ◽

2021 ◽

Vol 16 (6) ◽

pp. e0253173

Author(s):

Shobhna Mishra ◽

Gunjan Sharma ◽

Manoj K. Das ◽

Veena Pande ◽

Om P. Singh

Keyword(s):

Molecular Marker ◽

Ribosomal Dna ◽

Internal Transcribed Spacer ◽

Sequence Variation ◽

Anopheles Stephensi ◽

28S Rdna ◽

Reference Sequence ◽

Its2 Region ◽

Intragenomic Variation ◽

Second Internal Transcribed Spacer

Second Internal Transcribed Spacer (ITS2) ribosomal DNA (rDNA) sequence is a widely used molecular marker for species-identification or -delimitation due to observed concerted evolution which is believed to homogenize rDNA copies in an interbreeding population. However, intra-specific differences in ITS2 of Anopheles stephensi have been reported. This study reports the presence of intragenomic sequence variation in the ITS2-rDNA of An. stephensi and hypothesizes that observed intra-specific differences in this species may have resulted due to ambiguous DNA sequence-chromatogram resulting from intragenomic heterogeneity. Anopheles stephensi collected from different parts of India were sequenced for complete ITS2 and the variable region of 28S-rDNA (d1-d3 domains). Intragenomic variations were found in ITS2 region of all An. stephensi sequenced, but no such variation was observed in d1 to d3 domains of 28S-rDNA. Cloning and sequencing of ITS2 through the d3 domain of the 28S region of rDNA from representative samples from northern, central, and southern India confirmed the presence of intragenomic variation in ITS2 due to transitions at three loci and two bp indel in a di-nucleotide microsatellite locus. Multiple haplotypes were observed in ITS2 raised from such variations. Due to the absence of detectable intragenomic sequence variation in the d1 to d3 domain of 28S rDNA of An. stephensi, this region can serve as an ideal reference sequence for taxonomic and phylogenetic studies. The presence of intragenomic variation in rDNA should be carefully examined before using this as a molecular marker for species delimitation or phylogenetic analyses.

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First report of Fusarium proliferatum causing stem and root rot on lucky bamboo (Dracaena braunii) in Iraq

Hellenic Plant Protection Journal ◽

10.2478/hppj-2019-0001 ◽

2019 ◽

Vol 12 (1) ◽

pp. 1-5

Author(s):

A.A. Lahuf

Keyword(s):

Ribosomal Dna ◽

Root Rot ◽

Internal Transcribed Spacer ◽

Morphological Characteristics ◽

Its Region ◽

Fusarium Proliferatum ◽

Ornamental Plant ◽

Sequence Analyses ◽

First Report

Summary Lucky bamboo (Dracaena braunii) is a popular ornamental plant in Iraq. Individuals of this plant showing stem and root rot symptoms were observed during a survey conducted from November 2015 to February 2016 in several nurseries in Kerbala province, Iraq. Based on morphological characteristics and sequence analyses of the internal transcribed spacer (ITS) region of the ribosomal DNA (rDNA), the pathogen was identified as Fusarium proliferatum. This is the first report of stem and root rot caused by F. proliferatum on lucky bamboo (D. braunii) in Iraq.

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