scholarly journals Recycling of Solanum Sucrose Transporters Expressed in Yeast, Tobacco, and in Mature Phloem Sieve Elements

2010 ◽  
Vol 3 (6) ◽  
pp. 1064-1074 ◽  
Author(s):  
Johannes Liesche ◽  
Hong-Xia He ◽  
Bernhard Grimm ◽  
Alexander Schulz ◽  
Christina Kühn
Keyword(s):  
Sieve Elements ◽  
Sucrose Transporters

scholarly journals Role of the SUT1 and SUT2 sugar transporters during stolbur phytoplasma infection in tomato

2020 ◽  
Author(s):  
Federica De Marco ◽  
Brigitte Batailler ◽  
Michael R. Thorpe ◽  
Frédérique Razan ◽  
Rozenn Le Hir ◽  
...  
Keyword(s):  
Redox Homeostasis ◽  
Sugar Transport ◽  
Transcriptional Analysis ◽  
Starch Accumulation ◽  
Metabolic Switch ◽  
Sieve Elements ◽  
Sugar Transporters ◽  
Abnormal Growth ◽  
Sucrose Transporters ◽  
Phytoplasma Infection

SummaryPhytoplasmas inhabit phloem sieve elements and cause abnormal growth and altered sugar partitioning. But how they interact with phloem functions is not clearly known. The phloem responses were investigated in tomato infected by ‘Candidatus Phytoplasma solani’, at the beginning of the symptomatic stage of infection, both in symptomatic and asymptomatic leaves, the first symptoms appearing in the sink top leaf at the stem apex. Antisense lines impaired in the phloem sucrose transporters SUT1 and SUT2 were included. The infection in source leaves was not associated with symptoms. In the symptomatic, sink leaf, yellowing and leaf curling was associated with higher starch accumulation and expression of defense genes. The transcriptional analysis of symptomatic leaf midribs indicated that transcript levels for genes acting in the glycolysis and peroxisome metabolism in infected plants differed from these in non-infected plants. Phytoplasma multiplied actively in at least three additional lower leaves although they were symptomless, with no sign of activation of defense markers, although the rate of exudation of sucrose from these symptomless, source leaves was lower for infected plants. A few metabolites in phloem-enriched exudate were affected by the infection, such as glycolate and aspartate, and some of them were also affected in the control SUT1- and SUT2- antisense lines, in which sucrose retrieval or release in the sieve elements are impaired. A metabolic switch could explain the delivery of more glycolate into the sieve elements of infected plants. The findings suggest a link between sugar transport and redox homeostasis.One sentence summaryAn impairment of sucrose retrieval and release in the sieve elements occurs during phytoplasma infection, associated with changes in sugar and peroxisome metabolism

A New Subfamily of Sucrose Transporters, SUT4, with Low Affinity/High Capacity Localized in Enucleate Sieve Elements of Plants

2000 ◽  
Vol 12 (8) ◽  
pp. 1345 ◽  
Author(s):  
Andreas Weise ◽  
Laurence Barker ◽  
Christina Kuhn ◽  
Sylvie Lalonde ◽  
Henrik Buschmann ◽  
...  
Keyword(s):  
High Capacity ◽  
Sieve Elements ◽  
Sucrose Transporters

scholarly journals A New Subfamily of Sucrose Transporters, SUT4, with Low Affinity/High Capacity Localized in Enucleate Sieve Elements of Plants

2000 ◽  
Vol 12 (8) ◽  
pp. 1345-1355 ◽  
Author(s):  
Andreas Weise ◽  
Laurence Barker ◽  
Christina Kühn ◽  
Sylvie Lalonde ◽  
Henrik Buschmann ◽  
...  
Keyword(s):  
High Capacity ◽  
Sieve Elements ◽  
Sucrose Transporters

The Fine Structure of Phloem Cells

1985 ◽  
Vol 43 ◽  
pp. 632-635
Author(s):  
James Cronshaw
Keyword(s):  
Structural Studies ◽  
High Concentration ◽  
Long Distance ◽  
Phloem Tissue ◽  
Sieve Elements ◽  
Long Distance Transport ◽  
P Protein ◽  
Companion Cells ◽  
Electron Microscopical ◽  
Distance Transport

Long distance transport in plants takes place in phloem tissue which has characteristic cells, the sieve elements. At maturity these cells have sieve areas in their end walls with specialized perforations. They are associated with companion cells, parenchyma cells, and in some species, with transfer cells. The protoplast of the functioning sieve element contains a high concentration of sugar, and consequently a high hydrostatic pressure, which makes it extremely difficult to fix mature sieve elements for electron microscopical observation without the formation of surge artifacts. Despite many structural studies which have attempted to prevent surge artifacts, several features of mature sieve elements, such as the distribution of P-protein and the nature of the contents of the sieve area pores, remain controversial.

Freeze-Fracture Studies of Membranes in Developing Sieve Elements in a Plant Tissue Culture

1980 ◽  
Vol 38 ◽  
pp. 636-637
Author(s):  
R. D. Sjolund ◽  
C. Y. Shih
Keyword(s):  
Plant Tissue ◽  
Cultured Cells ◽  
Freeze Fracture ◽  
Tissue Cultures ◽  
Sieve Elements ◽  
Intact Plants ◽  
Plant Tissue Cultures ◽  
Whole Plants ◽  
Energy Dependent ◽  
Similar Elements

The differentiation of phloem in plant tissue cultures offers a unique opportunity to study the development and structure of sieve elements in a manner that avoids the injury responses associated with the processing of similar elements in intact plants. Short segments of sieve elements formed in tissue cultures can be fixed intact while the longer strands occuring in whole plants must be cut into shorter lengths before processing. While iyuch controversy surrounds the question of phloem function in tissue cultures , sieve elements formed in these cultured cells are structurally similar to those of Intact plants. We are particullarly Interested In the structure of the plasma membrane and the peripheral ER in these cells because of their possible role in the energy-dependent active transport of sucrose into the sieve elements.

Nondispersive Protein Bodies in Sieve Elements: A Survey and Review of their Origin, Distribution and Taxonomic Significance

IAWA Journal ◽  
1991 ◽  
Vol 12 (2) ◽  
pp. 143-175 ◽  
Author(s):  
H.-Dietmar Behnke
Keyword(s):  
Electron Microscope ◽  
Protein Bodies ◽  
Evolutionary Trends ◽  
Taxonomic Significance ◽  
Additional Species ◽  
Sieve Elements ◽  
P Protein ◽  
Taxonomic Range ◽  
Characteristic Features

Nondispersive protein bodies present in the sieve elements in addition to dispersive P-protein are characteristic features of many woody dicotyledons; their origin may be nuclear or cytoplasmic. While nuclear nondispersive protein bodies are found in only two families, the Boraginaceae and Myristicaceae, bodies of cytoplasmic origin are present in 39 of the more than 350 families screened. These results were obtained from 228 dicotyledons studied with the electron microscope and data of additional species from the literature. The terminology, origin, form and distribution of nondispersive protein bodies are discussed. Their ultrastructural composition is described as being predominantly spindle-shaped, compound- spherical, rod-shaped and rosette-like. Based on the data accumulated from over 450 species (of about 3000 screened) it is evident that their taxonomic range is confined to a few dicotyledon superorders. Compound-spherical nondispersive protein bodies are characteristic of most of the Malvanae/Violanae; spindle-shaped forms are restricted to the Fabaceae (Rutanae). Rosanae-Proteanae-Myrtanae and the Magnolianae are the only other superorders that contain nondispersive protein bodies in several of their families. Evolutionary trends and possible taxonomic consequences implied in this distribution are discussed.

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