scholarly journals Hybridization of synthetic oligodeoxyribonucleotides to ΦX174 DNA: the effect of single base pair mismatch

1979 ◽  
Vol 6 (11) ◽  
pp. 3543-3558 ◽  
Author(s):  
R. Bruce Wallace ◽  
J. Shaffer ◽  
R.F. Murphy ◽  
J. Bonner ◽  
T. Hirose ◽  
...  
2014 ◽  
Vol 86 (7) ◽  
pp. 3461-3467 ◽  
Author(s):  
Shuo Wu ◽  
Pingping Liang ◽  
Haixiang Yu ◽  
Xiaowen Xu ◽  
Yuan Liu ◽  
...  

2001 ◽  
Vol 123 (47) ◽  
pp. 11811-11812 ◽  
Author(s):  
Timothy S. Hall ◽  
Petr Pancoska ◽  
Peter V. Riccelli ◽  
Kathleen Mandell ◽  
Albert S. Benight

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Anustup Poddar ◽  
Muhammad S. Azam ◽  
Tunc Kayikcioglu ◽  
Maksym Bobrovskyy ◽  
Jichuan Zhang ◽  
...  

AbstractBase-pairing interactions mediate many intermolecular target recognition events. Even a single base-pair mismatch can cause a substantial difference in activity but how such changes influence the target search kinetics in vivo is unknown. Here, we use high-throughput sequencing and quantitative super-resolution imaging to probe the mutants of bacterial small RNA, SgrS, and their regulation of ptsG mRNA target. Mutations that disrupt binding of a chaperone protein, Hfq, and are distal to the mRNA annealing region still decrease the rate of target association, kon, and increase the dissociation rate, koff, showing that Hfq directly facilitates sRNA–mRNA annealing in vivo. Single base-pair mismatches in the annealing region reduce kon by 24–31% and increase koff by 14–25%, extending the time it takes to find and destroy the target by about a third. The effects of disrupting contiguous base-pairing are much more modest than that expected from thermodynamics, suggesting that Hfq buffers base-pair disruptions.


2003 ◽  
Vol 69 (5) ◽  
pp. 2848-2856 ◽  
Author(s):  
Hidetoshi Urakawa ◽  
Said El Fantroussi ◽  
Hauke Smidt ◽  
James C. Smoot ◽  
Erik H. Tribou ◽  
...  

ABSTRACT The discrimination between perfect-match and single-base-pair-mismatched nucleic acid duplexes was investigated by using oligonucleotide DNA microarrays and nonequilibrium dissociation rates (melting profiles). DNA and RNA versions of two synthetic targets corresponding to the 16S rRNA sequences of Staphylococcus epidermidis (38 nucleotides) and Nitrosomonas eutropha (39 nucleotides) were hybridized to perfect-match probes (18-mer and 19-mer) and to a set of probes having all possible single-base-pair mismatches. The melting profiles of all probe-target duplexes were determined in parallel by using an imposed temperature step gradient. We derived an optimum wash temperature for each probe and target by using a simple formula to calculate a discrimination index for each temperature of the step gradient. This optimum corresponded to the output of an independent analysis using a customized neural network program. These results together provide an experimental and analytical framework for optimizing mismatch discrimination among all probes on a DNA microarray.


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