scholarly journals The genome sequence of Xanthomonas oryzae pathovar oryzae KACC10331, the bacterial blight pathogen of rice

2005 ◽  
Vol 33 (2) ◽  
pp. 577-586 ◽  
Author(s):  
B.-M. Lee
Keyword(s):  
Genome Sequence ◽  
Bacterial Blight ◽  
Xanthomonas Oryzae ◽  
Bacterial Blight Pathogen

Biological control of rice bacterial blight by plant-associated bacteria producing 2,4-diacetylphloroglucinol

2006 ◽  
Vol 52 (1) ◽  
pp. 56-65 ◽  
Author(s):  
Palaniyandi Velusamy ◽  
J Ebenezar Immanuel ◽  
Samuel S Gnanamanickam ◽  
Linda Thomashow
Keyword(s):  
Biological Control ◽  
Pseudomonas Fluorescens ◽  
Bacterial Blight ◽  
Field Experiments ◽  
Screening Method ◽  
Xanthomonas Oryzae ◽  
Rice Bacterial Blight ◽  
Fluorescent Pseudomonas ◽  
Tn5 Mutants ◽  
Bacterial Blight Pathogen

Certain plant-associated strains of fluorescent Pseudomonas spp. are known to produce the antimicrobial antibiotic 2,4-diacetylphloroglucinol (DAPG). It has antibacterial, antifungal, antiviral, and antihelminthic properties and has played a significant role in the biological control of tobacco, wheat, and sugar beet diseases. It has never been reported from India and has not been implicated in the biological suppression of a major disease of the rice crop. Here, we report that a subpopulation of 27 strains of plant-associated Pseudomonas fluorescens screened in a batch of 278 strains of fluorescent pseudomonads produced DAPG. The DAPG production was detected by a PCR-based screening method that used primers Phl2a and Phl2b and amplified a 745-bp fragment characteristic of DAPG. HPLC,1H NMR, and IR analyses provided further evidence for its production. We report also that this compound inhibited the growth of the devastating rice bacterial blight pathogen Xanthomonas oryzae pv. oryzae in laboratory assays and suppressed rice bacterial blight up to 59%–64% in net-house and field experiments. Tn5 mutants defective in DAPG production (Phl–) of P. fluorescens PTB 9 were much less effective in their suppression of rice bacterial blight.Key words: biocontrol, 2,4-diacetylphloroglucinol, Pseudomonas fluorescens, rice, Xanthomonas oryzae pv. oryzae.

scholarly journals Antagonistic activity of rice rhizosphere isolates against Xanthomonas oryzae pv. oryzae bacterial blight pathogen

2021 ◽  
pp. 211-215
Author(s):  
Yamini sousheel N. ◽  
Bharati Narayan Bhat ◽  
Gouri Shankar Laha ◽  
S. Triveni
Keyword(s):  
Bacterial Blight ◽  
Antagonistic Activity ◽  
Xanthomonas Oryzae ◽  
Lesion Length ◽  
Pseudomonas Spp ◽  
Fluorescent Pseudomonas ◽  
Rice Rhizosphere ◽  
Bacillus Spp ◽  
Bacterial Blight Pathogen

Attempts were made to isolate beneficial bio agents from rice rhizosphere which resulted in isolation of  46 Bacillus spp and 15 fluorescent Pseudomonas spp which were further investigated for their potential aginst BB of rice diseaseAmong twenty six isolates of Bacillus, two isolates were most antagonistic and showed highest inhibition percentage (57.09) The potential isolates of Pseudomonas (P-4, P-5, P-6, P-7 and P-8), Bacillus (ARI 1-2, ARI 2-4, ARI 1-3, D1-1-2 and D2-1-1) were tested in vivo under glass house conditions for antagonism against Xoo which revealed that P-7 was effective in reduction of lesion length (18.5cm) when compared to control (20.37cm). The potential isolates of Pseudomonas (P-4, P-5, P-6, P-7 and P-8), Bacillus (ARI 1-2, ARI 2-4, ARI 1-3, D1-1-2 and D2-1-1) were tested in vivo under glass house conditions for antagonism against Xoo which revealed that P-7 was effective in reduction of lesion length (18.5cm) when compared to control (20.37cm). The present study indicated that PGPR isolates P-4, P-6, P-7 and P-8 can be used as biofertilizers, which will add up for enhanced growth of rice.   

scholarly journals Complete Genome Sequence of Bacillus altitudinis P-10, a Potential Bioprotectant against Xanthomonas oryzae pv. oryzae, Isolated from Rice Rhizosphere in Java, Indonesia

2017 ◽  
Vol 5 (48) ◽  
Author(s):  
Anto Budiharjo ◽  
Haeyoung Jeong ◽  
Dyah Wulandari ◽  
Soohyun Lee ◽  
Choong-Min Ryu
Keyword(s):  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Bacterial Blight ◽  
Rice Field ◽  
Xanthomonas Oryzae ◽  
Content Type ◽  
Bacillus Altitudinis ◽  
Rice Rhizosphere ◽  
Organic Rice

ABSTRACT Bacillus altitudinis P-10 was isolated from the rhizosphere of rice grown in an organic rice field and provides strong antagonism against the bacterial blight caused by Xanthomonas oryzae pv. oryzae in rice. Herein, we provide the complete genome sequence and a possible explanation of the antibiotic function of the P-10 strain.

scholarly journals A strain of an emerging Indian pathotype of Xanthomonas oryzae pv. oryzae defeats the rice bacterial blight resistance gene xa13 without inducing a clade III SWEET gene and is nearly identical to a recent Thai isolate

2018 ◽  
Author(s):  
Sara C. D. Carpenter ◽  
Prashant Mishra ◽  
Chandrika Ghoshal ◽  
Prasanta Dash ◽  
Li Wang ◽  
...  
Keyword(s):  
Resistance Genes ◽  
Bacterial Blight ◽  
Xanthomonas Oryzae ◽  
Host Susceptibility ◽  
Transcription Activator ◽  
Rice Bacterial Blight ◽  
Indian Strain ◽  
Bacterial Blight Pathogen ◽  
Insight Into ◽  
S Genes

AbstractThe rice bacterial blight pathogen Xanthomonas oryzae pv. oryzae (Xoo) injects transcription activator-like effectors (TALEs) that bind and activate host ‘susceptibility’ (S) genes important for disease. Clade III SWEET genes are major S genes for bacterial blight. The resistance genes xa5, which reduces TALE activity generally, and xa13, a SWEET11 allele not recognized by the cognate TALE, have been effectively deployed. However, strains that defeat both resistance genes individually were recently reported in India and Thailand. To gain insight into the mechanism(s), we completely sequenced the genome of one such strain from each country and examined the encoded TALEs. Strikingly, the two strains are clones, sharing nearly identical TALE repertoires, including a TALE known to activate SWEET11 strongly enough to be effective even when diminished by xa5. We next investigated SWEET gene induction by the Indian strain. The Indian strain induced no clade III SWEET in plants harbouring xa13, indicating a pathogen adaptation that relieves dependence on these genes for susceptibility. The findings open a door to mechanistic understanding of the role SWEET genes play in susceptibility and illustrate the importance of complete genome sequence-based monitoring of Xoo populations in developing varieties with effective disease resistance.

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