scholarly journals Establishment of single-cell screening system for the rapid identification of transcriptional modulators involved in direct cell reprogramming

2012 ◽  
Vol 40 (21) ◽  
pp. e165-e165 ◽  
Author(s):  
Jay W. Shin ◽  
Takahiro Suzuki ◽  
Noriko Ninomiya ◽  
Mami Kishima ◽  
Yuki Hasegawa ◽  
...  
Keyword(s):  
Single Cell ◽  
Rapid Identification ◽  
Cell Reprogramming ◽  
Screening System ◽  
Transcriptional Modulators ◽  
Direct Cell

Precise Integration of Direct Cell Reprogramming-Induced Neuronal Cells into Adult Neocortical Circuits

2018 ◽  
Author(s):  
Fengchao Wang ◽  
Yueguang Liu ◽  
Yutong Pang ◽  
Fei Liu ◽  
Xiang Li ◽  
...  
Keyword(s):  
Neuronal Cells ◽  
Cell Reprogramming ◽  
Precise Integration ◽  
Direct Cell

scholarly journals Quality control of direct cell–mineral adhesion measurements in air and liquid using inverse AFM imaging

RSC Advances ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 5384-5392
Author(s):  
Abd Alaziz Abu Quba ◽  
Gabriele E. Schaumann ◽  
Mariam Karagulyan ◽  
Doerte Diehl
Keyword(s):  
Quality Control ◽  
Single Cell ◽  
Single Cell Level ◽  
Cell Level ◽  
C Cell ◽  
Afm Imaging ◽  
Direct Cell ◽  
Mineral Interaction

Setup for a reliable cell-mineral interaction at the single-cell level, (a) study of the mineral by a sharp tip, (b) study of the bacterial modified probe by a characterizer, (c) cell-mineral interaction, (d) subsequent check of the modified probe.

scholarly journals RNA-guided cell targeting with CRISPR/RfxCas13d collateral activity in human cells

2021 ◽  
Author(s):  
Peiguo Shi ◽  
Michael R Murphy ◽  
Alexis O Aparicio ◽  
Jordan S Kesner ◽  
Zhou Fang ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Single Cell ◽  
State Transition ◽  
Cell Types ◽  
Rapid Identification ◽  
Human Cells ◽  
Single Cell Sequencing ◽  
Functional Studies ◽  
Single Marker ◽  
Inhibit Cell Proliferation

While single-cell sequencing has allowed rapid identification of novel cell types or states and associated RNA markers, functional studies remain challenging due to the lack of tools that are able to target specific cells based on these markers. Here we show that targeting a single marker RNA with CRISPR/RfxCas13d led to collateral transcriptome destruction in human cells, which can be harnessed to inhibit cell proliferation or to suppress cell state transition.

scholarly journals Direct cell reprogramming is a stochastic process amenable to acceleration

Nature ◽  
2009 ◽  
Vol 462 (7273) ◽  
pp. 595-601 ◽  
Author(s):  
Jacob Hanna ◽  
Krishanu Saha ◽  
Bernardo Pando ◽  
Jeroen van Zon ◽  
Christopher J. Lengner ◽  
...  
Keyword(s):  
Stochastic Process ◽  
Cell Reprogramming ◽  
Direct Cell

scholarly journals Resolving Cell Fate Decisions during Somatic Cell Reprogramming by Single-Cell RNA-Seq

2019 ◽  
Vol 73 (4) ◽  
pp. 815-829.e7 ◽  
Author(s):  
Lin Guo ◽  
Lihui Lin ◽  
Xiaoshan Wang ◽  
Mingwei Gao ◽  
Shangtao Cao ◽  
...  
Keyword(s):  
Somatic Cell ◽  
Single Cell ◽  
Cell Fate ◽  
Cell Reprogramming ◽  
Rna Seq ◽  
Cell Fate Decisions ◽  
Somatic Cell Reprogramming

scholarly journals Alcohol‐tolerant mutants of cyanobacterium Synechococcus elongatus PCC 7942 obtained by single‐cell mutant screening system

2017 ◽  
Vol 114 (8) ◽  
pp. 1771-1778 ◽  
Author(s):  
Sayuri Arai ◽  
Kayoko Hayashihara ◽  
Yuki Kanamoto ◽  
Kazunori Shimizu ◽  
Yasutaka Hirokawa ◽  
...  
Keyword(s):  
Single Cell ◽  
Synechococcus Elongatus ◽  
Screening System ◽  
Cell Mutant ◽  
Mutant Screening ◽  
Synechococcus Elongatus Pcc 7942 ◽  
Pcc 7942

Comparative Study of AFM and FESEM for Imaging the Single Cell of Escherichia Coli Bacteria

2015 ◽  
Vol 34 ◽  
pp. 61-66
Author(s):  
Kamarulazizi Ibrahim ◽  
Mohammad Hafiz Khalid ◽  
Mohamed Hassan Eisa ◽  
Mohd Nazalan Najimudin ◽  
Mohammad A. Al Rajhi ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Comparative Study ◽  
Single Cell ◽  
Food Poisoning ◽  
Rapid Identification ◽  
Force Microscopy ◽  
E Coli ◽  
Atomic Force ◽  
Identification Of Bacteria ◽  
Escherichia Coli Bacteria

In this work, a comparative study using atomic force microscopy (AFM) and field emission–scanning electron microscopy (FESEM) has been carried out to assess the morphology of single cellEscherichia colibacteria (E-coli).E-colibacteria are a major concern for public health. Attention was focused on the certain strains ofE-colibacteria, because some strains can be toxic and cause food poisoning. TheE-colibacteria have attracted much research interest because this bacterium is easily to get, cheap and rapid reproductively. Imaging ofE-colirecently, was improved by using high resolution microscopy. Current techniques for detection such as, AFM and FESEM has attracted great interest and emerging as a potentially powerful whole-organism fingerprinting tool for the rapid identification of bacteria. The obtained results of AFM and FESEM techniques have been compared to show the image quality of single cellE-coli.

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