Functional characterization of C. elegans Y-box-binding proteins reveals tissue-specific functions and a critical role in the formation of polysomes

AbstractThe sgs-1 (suppressor of activated Gαs) gene encodes one of the four adenylyl cyclases in the nematode C. elegans and is most similar to mammalian adenylyl cyclase type IX. We isolated a complete loss-of-function mutation in sgs-1 and found it to result in animals with retarded development that arrest in variable larval stages. sgs-1 mutant animals exhibit lethargic movement and pharyngeal pumping and (while not reaching adulthood) have a mean life span that is >50% extended compared to wild type. An extensive set of reduction-of-function mutations in sgs-1 was isolated in a screen for suppressors of a neuronal degeneration phenotype induced by the expression of a constitutively active version of the heterotrimeric Gαs subunit of C. elegans. Although most of these mutations change conserved residues within the catalytic domains of sgs-1, mutations in the less-conserved transmembrane domains are also found. The sgs-1 reduction-of-function mutants are viable and have reduced locomotion rates, but do not show defects in pharyngeal pumping or life span.

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Identification and functional characterization of odorant-binding proteins 69a and 76a of Drosophila suzukii

Heliyon ◽

10.1016/j.heliyon.2021.e06427 ◽

2021 ◽

Vol 7 (3) ◽

pp. e06427

Author(s):

Haixia Zhan ◽

Du Li ◽

Youssef Dewer ◽

Changying Niu ◽

Fengqi Li ◽

...

Keyword(s):

Binding Proteins ◽

Functional Characterization ◽

Drosophila Suzukii ◽

Odorant Binding Proteins ◽

Odorant Binding

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Structural and functional characterization of the receptor binding proteins of Escherichia coli O157 phages EP75 and EP335

Computational and Structural Biotechnology Journal ◽

10.1016/j.csbj.2021.06.001 ◽

2021 ◽

Author(s):

Sander Witte ◽

Léa V. Zinsli ◽

Rafael Gonzalez-Serrano ◽

Cassandra I. Matter ◽

Martin J. Loessner ◽

...

Keyword(s):

Escherichia Coli ◽

Receptor Binding ◽

Binding Proteins ◽

Functional Characterization ◽

Escherichia Coli O157

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Functional characterization of odorant-binding proteins from the scarab beetle Holotrichia oblita based on semiochemical-induced expression alteration and gene silencing

Insect Biochemistry and Molecular Biology ◽

10.1016/j.ibmb.2018.11.002 ◽

2019 ◽

Vol 104 ◽

pp. 11-19 ◽

Cited By ~ 13

Author(s):

Jiao Yin ◽

Chaoqun Wang ◽

Chiqin Fang ◽

Shuai Zhang ◽

Yazhong Cao ◽

...

Keyword(s):

Gene Silencing ◽

Binding Proteins ◽

Functional Characterization ◽

Scarab Beetle ◽

Induced Expression ◽

Odorant Binding Proteins ◽

Odorant Binding

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Functional Characterization of a New Class of Odorant-Binding Proteins in the MothMamestra brassicae

Biochemical and Biophysical Research Communications ◽

10.1006/bbrc.1998.9806 ◽

1998 ◽

Vol 253 (2) ◽

pp. 489-494 ◽

Cited By ~ 52

Author(s):

Jonathan Bohbot ◽

Franck Sobrio ◽

Philippe Lucas ◽

Patricia Nagnan-Le Meillour

Keyword(s):

Binding Proteins ◽

Functional Characterization ◽

Odorant Binding Proteins ◽

New Class ◽

Odorant Binding

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Functional characterization of an adhesive component from the embryonic chick neural retina

Journal of Cell Science ◽

10.1242/jcs.36.1.323 ◽

1979 ◽

Vol 36 (1) ◽

pp. 323-342

Author(s):

R. Rutz ◽

J. Lilien

Keyword(s):

Conditioned Medium ◽

Ph Dependence ◽

Functional Characterization ◽

Surface Binding ◽

Cell Agglutination ◽

Tissue Specific ◽

A Cell ◽

Surface Ligand ◽

Fixed Cell

We have developed a quantitative assay for tissue-specific adhesive components which is based on the agglutination of glutaraldehyde-fixed cells. At least 2 components are required for fixed-cell agglutination: a cell-surface ligand which is obtained from tissue culture-conditioned medium, and a soluble ‘agglutinin’ which accumulates in conditioned medium from monolayer cultures. Our results suggest that the surface-binding ligand and the agglutinin interact directly, resulting in tissue-specific agglutination of cells. The agglutination reaction exhibits divalent cation, temperature, and pH dependence. Several models of cell adhesion are described; the simplest of these which can account for the data is a multicomponent model in which the 2 adhesive components have structural roles.

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