scholarly journals Transforming growth factor- 1 decreases epithelial sodium channel functionality in renal collecting duct cells via a Smad4-dependent pathway

2007 ◽  
Vol 23 (4) ◽  
pp. 1126-1134 ◽  
Author(s):  
C.-T. Chang ◽  
C.-C. Hung ◽  
Y.-C. Chen ◽  
T.-H. Yen ◽  
M.-S. Wu ◽  
...  
Keyword(s):  
Growth Factor ◽  
Sodium Channel ◽  
Transforming Growth Factor ◽  
Collecting Duct ◽  
Epithelial Sodium Channel ◽  
Duct Cells ◽  
Collecting Duct Cells ◽  
Renal Collecting Duct ◽  
Dependent Pathway

scholarly journals Extracellular vesicles regulate purinergic signaling and epithelial sodium channel expression in renal collecting duct cells

2021 ◽  
Vol 35 (5) ◽  
Author(s):  
Eric R. Barros Lamus ◽  
Valentina Carotti ◽  
Christine R. S. Vries ◽  
Femke Witsel ◽  
Onno J. Arntz ◽  
...  
Keyword(s):  
Sodium Channel ◽  
Extracellular Vesicles ◽  
Purinergic Signaling ◽  
Collecting Duct ◽  
Epithelial Sodium Channel ◽  
Duct Cells ◽  
Channel Expression ◽  
Collecting Duct Cells ◽  
Renal Collecting Duct

scholarly journals The Epithelial Sodium Channel (ENaC) Traffics to Apical Membrane in Lipid Rafts in Mouse Cortical Collecting Duct Cells

2007 ◽  
Vol 282 (52) ◽  
pp. 37402-37411 ◽  
Author(s):  
Warren G. Hill ◽  
Michael B. Butterworth ◽  
Huamin Wang ◽  
Robert S. Edinger ◽  
Jonathan Lebowitz ◽  
...  
Keyword(s):  
Sodium Channel ◽  
Lipid Rafts ◽  
Apical Membrane ◽  
Collecting Duct ◽  
Epithelial Sodium Channel ◽  
Cortical Collecting Duct ◽  
Duct Cells ◽  
Collecting Duct Cells ◽  
Epithelial Sodium Channel Enac

Epidermal growth factor inhibits amiloride-sensitive sodium absorption in renal collecting duct cells

2003 ◽  
Vol 284 (1) ◽  
pp. F57-F64 ◽  
Author(s):  
Jie-Pan Shen ◽  
Calvin U. Cotton
Keyword(s):  
Growth Factor ◽  
Chronic Exposure ◽  
Collecting Duct ◽  
Short Circuit ◽  
Short Circuit Current ◽  
Mrna Levels ◽  
Erk Pathway ◽  
Duct Cells ◽  
Collecting Duct Cells ◽  
Renal Collecting Duct

The effects of the ERK pathway on electrogenic transepithelial Na+ absorption by renal collecting duct cells were determined. Approximately 90% of the unstimulated short-circuit current (15 ± 1 μA/cm2, n = 10) across conditionally immortalized murine collecting duct epithelial cells (mCT1) is amiloride sensitive and is likely mediated by apical epithelial Na+ channels. Chronic exposure (24 h) of the epithelial monolayers to either EGF (50 ng/ml) or transforming growth factor-α (TGF-α; 20 ng/ml) reduced amiloride-sensitive short-circuit current by >60%. The inhibitory effect of EGF on Na+ absorption was not due to inhibition of basolateral Na+-K+-ATPase, because the pump current elicited by permeabilization of apical membrane with nystatin was not reduced by EGF. Chronic exposure of the mCT1 cells to EGF (20 ng/ml, 24 h) elicited a 70–85% decrease in epithelial Na+ channel subunit mRNA levels. Exposure of mCT1 cells to either EGF (20 ng/ml) or PMA (150 nM) induced rapid phosphorylation of p42/p44 (ERK1/2) and pretreatment of the monolayers with PD-98059 (an ERK kinase inhibitor; 30 μM) prevented phosphorylation of p42/p44. Similarly, pretreatment of mCT1 monolayers with PD-98059 prevented the EGF- and PMA-induced inhibition of amiloride-sensitive Na+ absorption. The results of these studies demonstrate that amiloride-sensitive Na+ absorption by renal collecting duct cells is regulated by the ERK pathway. This pathway may play a role in alterations in ion transport that occur in polycystic kidney disease.

scholarly journals Resveratrol Inhibits the Epithelial Sodium Channel via Phopshoinositides and AMP-Activated Protein Kinase in Kidney Collecting Duct Cells

PLoS ONE ◽  
2013 ◽  
Vol 8 (10) ◽  
pp. e78019 ◽  
Author(s):  
Kelly M. Weixel ◽  
Allison Marciszyn ◽  
Rodrigo Alzamora ◽  
Hui Li ◽  
Oliver Fischer ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Sodium Channel ◽  
Collecting Duct ◽  
Epithelial Sodium Channel ◽  
Kidney Collecting Duct ◽  
Duct Cells ◽  
Collecting Duct Cells ◽  
Amp Activated Protein Kinase
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