scholarly journals Replication stress drives constitutive activation of the DNA damage response and consequent radioresistance in glioblastoma cancer stem cells

2018 ◽  
Vol 20 (suppl_1) ◽  
pp. i3-i4
Author(s):  
Ross Carruthers ◽  
Shafiq Ahmed ◽  
Shaliny Ramachandran ◽  
Ester Hammond ◽  
Anthony Chalmers
Author(s):  
Maria Louka ◽  
Effrossyni Boutou ◽  
Vasiliki Bakou ◽  
Vassiliki Pappa ◽  
Anastasios Georgoulis ◽  
...  

Oncogene ◽  
2019 ◽  
Vol 38 (17) ◽  
pp. 3103-3118 ◽  
Author(s):  
Ayala Gold ◽  
Lital Eini ◽  
Malka Nissim-Rafinia ◽  
Ruth Viner ◽  
Shlomit Ezer ◽  
...  

2020 ◽  
Vol 122 (2) ◽  
pp. 301-302
Author(s):  
Yulin Chen ◽  
Dan Li ◽  
Dapeng Wang ◽  
Xuefeng Liu ◽  
Ning Yin ◽  
...  

2019 ◽  
Vol 2019 ◽  
pp. 1-10 ◽  
Author(s):  
Heriberto Abraham Valencia-González ◽  
Graciela Ruíz ◽  
Elizabeth Ortiz-Sánchez ◽  
Alejandro García-Carrancá

Recently, a subpopulation of tumor cells, called cancer stem cells (CSC), has been characterized, and these have emerged as a major topic in cancer research. CSC are proposed to repair DNA damage more efficiently than the rest of tumor cells, resisting chemotherapy or radiotherapy and causing clinical recurrence and metastasis. We aimed to determine the molecular basis of radioresistance and first compared the response to ionizing radiation (IR) between cancer stem cell-enriched cultures grown as spheres and conventional tumor cell line cultures grown as monolayer, from HeLa and MCF-7 cancer cell lines. To verify that our sphere cultures were enriched in CSC, we evaluated the double staining of CD49f and ALDH activity for HeLa cells by flow cytometry. We then evaluated whether differences could exist in sensor elements in the DNA damage response pathway among these cultures. We found that CSC cultures showed less sensitivity to radiation than conventional tumor cell line cultures. We observed a higher baseline expression of activated response sensor proteins of DNA damage, such as ATM, H2A.X, and PARP1, in untreated CSC cultures. These findings provide the first evidence, to our knowledge, that DNA damage response sensor proteins are present and preferentially activated in CSC, as opposed to the bulk of cells in monolayer cultures. Likewise, they provide the basis for biological differences in response to IR between CSC and other tumor cell populations. Understanding the DNA damage response pathway may provide therapeutic targets to sensitize CSC to cytotoxic therapies to improve current cancer treatments.


2011 ◽  
Vol 8 (1) ◽  
pp. 16-29 ◽  
Author(s):  
Cedric Blanpain ◽  
Mary Mohrin ◽  
Panagiota A. Sotiropoulou ◽  
Emmanuelle Passegué

2012 ◽  
Vol 113 (12) ◽  
pp. 3643-3652 ◽  
Author(s):  
Yulin Chen ◽  
Dan Li ◽  
Dapeng Wang ◽  
Xuefeng Liu ◽  
Ning Yin ◽  
...  

MedChemComm ◽  
2017 ◽  
Vol 8 (2) ◽  
pp. 295-319 ◽  
Author(s):  
Cyril Ronco ◽  
Anthony R. Martin ◽  
Luc Demange ◽  
Rachid Benhida

A review highlighting on cancer stem cells, with an exhaustive listing and comparison of biological efficacies and pharmacology of the inhibitors of 5 pivotal enzymes of the DNA-damage response.


2020 ◽  
Vol 474 ◽  
pp. 106-117 ◽  
Author(s):  
Etna Abad ◽  
Dmitry Graifer ◽  
Alex Lyakhovich

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