Changes in the expression of adhesion molecules as peripheral blood monocytes differentiate into peritoneal macrophages

Abstract The effect of azathioprine on the kinetics of peripheral blood monocytes and peritoneal macrophages was studied in normal mice and in mice in which an inflammatory reaction was provoked. Two dosage levels were used: a high dose of 200mg/kg which is the maximum tolerated daily dose in mice, and low dose of 3 mg/kg which is about equivalent to a nontoxic, immunosuppressive, anti-inflammatory dose in man. The number of peripheral blood monocytes decreases gradually during azathioprine treatment of normal mice, the extent and duration being dependent on the dose and duration of administered over a period of 9 days gives an almost complete reduction, and a low dose (3 mg/kg) given for the same period results in a reduction of about 50%. This effect seems to be reversible, because when treatment is stopped the number of monocytes starts to increase 24–48 hr later. The number of peritoneal macrophages is only affected when a high dose (200 mg/kg) is given over a long period; a low dose has virtually no effect. In mice in which an inflammatory reaction was prevoked in the peritoneal cavity, the normally occurring increase in the numbers of both peripheral blood monocytes and peritoneal macrophages was suppressed, the extent being dependent on the dose of azathioprine administered. Labeling studies with 3H-thymidine indicated that the reduction of peripheral blood monocytes and peritoneal macrophages in the inflammatory exudate is due to a diminished monocyte production.

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Evaluation of the macrophages functional assay from different sources: Peritoneal macrophages, peripheral blood monocytes, and RAW 264.7 cells

Toxicology Letters ◽

10.1016/j.toxlet.2015.08.656 ◽

2015 ◽

Vol 238 (2) ◽

pp. S222

Author(s):

N.H. Kim ◽

S.M. Lee ◽

J.H. Lee ◽

K.D. Kim ◽

J.D. Heo

Keyword(s):

Peripheral Blood ◽

Peritoneal Macrophages ◽

Raw 264.7 Cells ◽

Functional Assay ◽

Raw 264.7 ◽

Blood Monocytes ◽

Peripheral Blood Monocytes ◽

Different Sources

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The effect of azathioprine (Imuran) on the kinetics of monocytes and macrophages during the normal steady state and an acute inflammatory reaction

Blood ◽

10.1182/blood.v46.1.51.bloodjournal46151 ◽

1975 ◽

Vol 46 (1) ◽

pp. 51-64 ◽

Cited By ~ 2

Author(s):

AE Gassmann ◽

R van Furth

Keyword(s):

Inflammatory Reaction ◽

Peripheral Blood ◽

Low Dose ◽

Peritoneal Macrophages ◽

High Dose ◽

Blood Monocytes ◽

Peripheral Blood Monocytes ◽

Azathioprine Treatment ◽

Monocytes And Macrophages ◽

Kinetics Of

The effect of azathioprine on the kinetics of peripheral blood monocytes and peritoneal macrophages was studied in normal mice and in mice in which an inflammatory reaction was provoked. Two dosage levels were used: a high dose of 200mg/kg which is the maximum tolerated daily dose in mice, and low dose of 3 mg/kg which is about equivalent to a nontoxic, immunosuppressive, anti-inflammatory dose in man. The number of peripheral blood monocytes decreases gradually during azathioprine treatment of normal mice, the extent and duration being dependent on the dose and duration of administered over a period of 9 days gives an almost complete reduction, and a low dose (3 mg/kg) given for the same period results in a reduction of about 50%. This effect seems to be reversible, because when treatment is stopped the number of monocytes starts to increase 24–48 hr later. The number of peritoneal macrophages is only affected when a high dose (200 mg/kg) is given over a long period; a low dose has virtually no effect. In mice in which an inflammatory reaction was prevoked in the peritoneal cavity, the normally occurring increase in the numbers of both peripheral blood monocytes and peritoneal macrophages was suppressed, the extent being dependent on the dose of azathioprine administered. Labeling studies with 3H-thymidine indicated that the reduction of peripheral blood monocytes and peritoneal macrophages in the inflammatory exudate is due to a diminished monocyte production.

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Studies on the Fc receptor of human peripheral blood monocytes and peritoneal macrophages: possible evidence for inducibility

Leukocyte Membrane Determinants Regulating Immune Reactivity ◽

10.1016/b978-0-12-233750-5.50044-0 ◽

1976 ◽

pp. 239

Author(s):

D.G. ROMANS ◽

K.J. DORRINGTON ◽

R.E. FALK

Keyword(s):

Peripheral Blood ◽

Human Peripheral Blood ◽

Peritoneal Macrophages ◽

Fc Receptor ◽

Blood Monocytes ◽

Peripheral Blood Monocytes

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THE EFFECT OF GLUCOCORTICOSTEROIDS ON THE KINETICS OF MONONUCLEAR PHAGOCYTES

Journal of Experimental Medicine ◽

10.1084/jem.131.3.429 ◽

1970 ◽

Vol 131 (3) ◽

pp. 429-442 ◽

Cited By ~ 155

Author(s):

Jan Thompson ◽

Ralph van Furth

Keyword(s):

Peritoneal Cavity ◽

Peripheral Blood ◽

Peritoneal Macrophages ◽

Rapid Decrease ◽

Mononuclear Phagocytes ◽

Water Soluble ◽

Hydrocortisone Acetate ◽

Blood Monocytes ◽

Peripheral Blood Monocytes ◽

Kinetics Of

The effect of glucocorticosteroids on the kinetics of mononuclear phagocytes, i.e., peripheral blood monocytes and peritoneal macrophages, was studied in normal mice, as well as in mice in which an inflammatory reaction was evoked in the peritoneal cavity. The administration of glucocorticosteroids resulted in a rapid decrease (within 3–6 hr) in the number of circulating monocytes, the duration being dependent on the nature and dose of the compound. The water-soluble dexamethasone sodium phosphate is only briefly active (less than 12 hr), but hydrocortisone acetate, which forms a subcutaneous depot, reduced the number of monocytes for more than 2 wk. In normal mice, hydrocortisone did not affect the number of macrophages already present in the peritoneal cavity, but the transit of mononuclear phagocytes from the circulation into the peritoneal cavity was arrested. During an inflammatory response in the peritoneal cavity, hydrocortisone suppresses both the increase in the number of monocytes in the peripheral blood and the increase in the number of peritoneal macrophages. This reduction of the inflammatory exudate appeared to be due to a diminished influx of mononuclear phagocytes from the peripheral blood. No lytic action of glucocorticosteroids on the mononuclear phagocytes could be demonstrated.

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