Objective: The main aim of this study was to evaluate the antioxidants present in Sesbania grandiflora (L.) Pers. belongs to the family Fabaceae.Methods: Fresh samples were used for the analysis of antioxidants such as total phenol, carotenoids, Vitamin-A, Vitamin-C, Vitamin-E, peroxidase (POD), catalase (CAT), superoxide dismutase (SOD), ascorbate peroxidase, monodehydroascorbate reductase, and glutathione reductase by standard estimation methods.Results: Present studies revealed that this wild leafy plant has numerous antioxidant factors that destroying the free radicals that damage the cells.Conclusion: S. grandiflora contain many enzymatic and non-enzymatic antioxidants and could be a good source of dietary antioxidants which play an important role in the prevention of diseases associated with oxidative stress.