scholarly journals Preparation and characterization of methacrylated gelatin/bacterial cellulose composite hydrogels for cartilage tissue engineering

2019 ◽  
Vol 7 (2) ◽  
pp. 195-202 ◽  
Author(s):  
Liling Gu ◽  
Tao Li ◽  
Xiongbo Song ◽  
Xianteng Yang ◽  
Senlei Li ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bacterial Cellulose ◽  
Articular Chondrocytes ◽  
Cartilage Tissue Engineering ◽  
Cartilage Tissue ◽  
X Ray Diffraction ◽  
Porous Network ◽  
Composite Hydrogels ◽  
Chondrocytic Phenotype

Abstract Methacrylated gelatin (GelMA)/bacterial cellulose (BC) composite hydrogels have been successfully prepared by immersing BC particles in GelMA solution followed by photo-crosslinking. The morphology of GelMA/BC hydrogel was examined by scanning electron microscopy and compared with pure GelMA. The hydrogels had very well interconnected porous network structure, and the pore size decreased from 200 to 10 µm with the increase of BC content. The composite hydrogels were also characterized by swelling experiment, X-ray diffraction, thermogravimetric analysis, rheology experiment and compressive test. The composite hydrogels showed significantly improved mechanical properties compared with pure GelMA. In addition, the biocompatility of composite hydrogels were preliminarily evaluated using human articular chondrocytes. The cells encapsulated within the composite hydrogels for 7 days proliferated and maintained the chondrocytic phenotype. Thus, the GelMA/BC composite hydrogels might be useful for cartilage tissue engineering.

Characterization of Chitosan-Based Scaffolds Seeded with Sheep Nasal Chondrocytes for Cartilage Tissue Engineering

2021 ◽  
Author(s):  
Anamarija Rogina ◽  
Maja Pušić ◽  
Lucija Štefan ◽  
Alan Ivković ◽  
Inga Urlić ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cartilage Tissue Engineering ◽  
Cartilage Tissue

scholarly journals In vitro characterization of a novel magnetic fibrin-agarose hydrogel for cartilage tissue engineering

2020 ◽  
Vol 104 ◽  
pp. 103619 ◽  
Author(s):  
Ana Belén Bonhome-Espinosa ◽  
Fernando Campos ◽  
Daniel Durand-Herrera ◽  
José Darío Sánchez-López ◽  
Sébastien Schaub ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cartilage Tissue Engineering ◽  
Cartilage Tissue ◽  
In Vitro Characterization ◽  
Agarose Hydrogel

scholarly journals Study of bacterial cellulose as scaffold on cartilage tissue engineering

2018 ◽  
Author(s):  
Saharman Gea ◽  
Reka Mustika Sari ◽  
Averroes Fazlurrahman Piliang ◽  
Denny Pratama Indrawan ◽  
Yasir Arafat Hutapea
Keyword(s):  
Tissue Engineering ◽  
Bacterial Cellulose ◽  
Cartilage Tissue Engineering ◽  
Cartilage Tissue

scholarly journals Structural and mechanical characterization of crosslinked and sterilised nanocellulose-based hydrogels for cartilage tissue engineering

2019 ◽  
Vol 212 ◽  
pp. 242-251 ◽  
Author(s):  
Ayesha Al-Sabah ◽  
Stephanie E.A. Burnell ◽  
Irina N. Simoes ◽  
Zita Jessop ◽  
Nafiseh Badiei ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Mechanical Characterization ◽  
Cartilage Tissue Engineering ◽  
Cartilage Tissue

scholarly journals Bioprinting of a Zonal-Specific Cell Density Scaffold: A Biomimetic Approach for Cartilage Tissue Engineering

2021 ◽  
Vol 11 (17) ◽  
pp. 7821
Author(s):  
Angeliki Dimaraki ◽  
Pedro J. Díaz-Payno ◽  
Michelle Minneboo ◽  
Mahdiyeh Nouri-Goushki ◽  
Maryam Hosseini ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Articular Cartilage ◽  
Cell Density ◽  
Articular Chondrocytes ◽  
Cartilage Tissue Engineering ◽  
Smooth Transition ◽  
Cartilage Tissue ◽  
Cartilage Defects ◽  
Specific Cell ◽  
Cell Densities

The treatment of articular cartilage defects remains a significant clinical challenge. This is partially due to current tissue engineering strategies failing to recapitulate native organization. Articular cartilage is a graded tissue with three layers exhibiting different cell densities: the superficial zone having the highest density and the deep zone having the lowest density. However, the introduction of cell gradients for cartilage tissue engineering, which could promote a more biomimetic environment, has not been widely explored. Here, we aimed to bioprint a scaffold with different zonal cell densities to mimic the organization of articular cartilage. The scaffold was bioprinted using an alginate-based bioink containing human articular chondrocytes. The scaffold design included three cell densities, one per zone: 20 × 106 (superficial), 10 × 106 (middle), and 5 × 106 (deep) cells/mL. The scaffold was cultured in a chondrogenic medium for 25 days and analyzed by live/dead assay and histology. The live/dead analysis showed the ability to generate a zonal cell density with high viability. Histological analysis revealed a smooth transition between the zones in terms of cell distribution and a higher sulphated glycosaminoglycan deposition in the highest cell density zone. These findings pave the way toward bioprinting complex zonal cartilage scaffolds as single units, thereby advancing the translation of cartilage tissue engineering into clinical practice.

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