scholarly journals Real-Time Polymerase Chain Reaction Analysis of CYP1B1 Gene Expression in Human Liver

2003 ◽  
Vol 71 (1) ◽  
pp. 11-19 ◽  
Author(s):  
T. K. H. Chang
Keyword(s):  
Gene Expression ◽  
Polymerase Chain Reaction ◽  
Real Time ◽  
Human Liver ◽  
Polymerase Chain Reaction Analysis ◽  
Chain Reaction ◽  
Cyp1b1 Gene ◽  
Polymerase Chain ◽  
Cyp1b1 Gene Expression

Comparison of reference genes for quantitative real-time polymerase chain reaction analysis of gene expression in sugarcane

2004 ◽  
Vol 22 (4) ◽  
pp. 325-337 ◽  
Author(s):  
Hayati M. Iskandar ◽  
Robert S. Simpson ◽  
Rosanne E. Casu ◽  
Graham D. Bonnett ◽  
Donald J. Maclean ◽  
...  
Keyword(s):  
Gene Expression ◽  
Polymerase Chain Reaction ◽  
Real Time ◽  
Reference Genes ◽  
Polymerase Chain Reaction Analysis ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Optimized QRT-PCR Approach for the Measurable Impact of Adjuvant Cholecalciferol Therapy in Ameliorating Cytokine Gene Expression

2021 ◽  
Vol 3 (6) ◽  
pp. 44-50
Author(s):  
Javed Akram ◽  
Akram Tariq ◽  
Gibran Ali ◽  
Fridoon Jawad Ahmed ◽  
Syeda Saba Aslam
Keyword(s):  
Gene Expression ◽  
Vitamin D ◽  
Polymerase Chain Reaction ◽  
Real Time ◽  
Polymerase Chain Reaction Analysis ◽  
Cytokine Gene Expression ◽  
Cytokine Gene ◽  
Chain Reaction ◽  
Open Label ◽  
Polymerase Chain

The endemic Vitamin D deficiency in Pakistan and the current COVID-19 epidemic have converged into a double whammy scenario in Pakistan [1]. Nutritional epigenomic studies have highlighted Vitamin D as a master Vitamin influencing various genomic expressions through its active metabolite 1α,25-dihydroxyvitamin D3 [2]. The objective of this study was to evaluate the measurable impact of adjuvant Cholecalciferol therapy in the Cytokine gene expression of COVID-19 patients by quantitative Real-Time Polymerase Chain Reaction analysis. The trial was a randomized control prospective open label interventional trial done on moderate to severe COVID-19 patients with deranged inflammatory and coagulation biomarkers. SunnyD STAT (Vitamin D3 200000 IU) softgels were given at Day 1, Day 3 and Day 5 of the treatment. Optimized quantitative Real-Time Polymerase Chain Reaction analysis showed decreased genetic expressions of Interleukin 6 (IL-6), Interleukin 2RA (IL-2RA) and Tumor Necrosis Factor (TNF-a) in the interventional group against the age and co-morbidities matched controls, providing molecular and genetic level evidence for the purported mechanism of amelioration of Cytokines induced pathogenic inflammation. However, inherent limitations of the design restrict the generalizability of the results and warrants caution for extrapolation. We recommend randomized placebo-controlled trials with larger sampling and genome wide profiling to infer more definite interpretations.

scholarly journals Expression of DROSHA and DICER genes in peripheral blood leukocytes in lung sarcoidosis

2018 ◽  
Vol 90 (3) ◽  
pp. 21-24
Author(s):  
I E Malysheva ◽  
O V Balan ◽  
E L Tikhonovich ◽  
T O Volkova
Keyword(s):  
Gene Expression ◽  
Polymerase Chain Reaction ◽  
Real Time ◽  
Peripheral Blood ◽  
Messenger Rna ◽  
Peripheral Blood Leukocytes ◽  
Chain Reaction ◽  
Blood Leukocytes ◽  
Healthy Donors ◽  
Polymerase Chain

Aim. To study the expression level of the genes DROSHA and DICER in peripheral blood leukocytes (PBL) of patients with sarcoidosis of the lungs Materials and methods. The study included 32 patients diagnosed with persistent lung sarcoidosis (mean age 41.56±1.27 years) and 36 healthy donors (control; mean age 42.79±1.95 years). The level of expression of messenger RNA (mRNA) of the genes DROSHA and DICER were determined in PBL of healthy donors and patients with sarcoidosis of the lung by polymerase chain reaction in real time. Results. As a result of the conducted researches it is established that the level of drosha gene expression in PBL patients with sarcoidosis of lungs is significantly reduced in comparison with the control (p

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