scholarly journals Dioxin Exposure Blocks Lactation through a Direct Effect on Mammary Epithelial Cells Mediated by the Aryl Hydrocarbon Receptor Repressor

2014 ◽  
Vol 143 (1) ◽  
pp. 36-45 ◽  
Author(s):  
Kaitlin J. Basham ◽  
Christopher J. Leonard ◽  
Collin Kieffer ◽  
Dawne N. Shelton ◽  
Maria E. McDowell ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Aryl Hydrocarbon Receptor ◽  
Direct Effect ◽  
Mammary Epithelial Cells ◽  
Mammary Epithelial ◽  
Aryl Hydrocarbon ◽  
Dioxin Exposure

Modulation of CYP1B1 and CYP1A1 gene expression and activation of aryl hydrocarbon receptor by Ginkgo biloba extract in MCF-10A human mammary epithelial cells

2009 ◽  
Vol 87 (9) ◽  
pp. 674-683 ◽  
Author(s):  
Ganesh Rajaraman ◽  
Guixiang Yang ◽  
Jie Chen ◽  
Thomas K.H. Chang
Keyword(s):  
Gene Expression ◽  
Epithelial Cells ◽  
Aryl Hydrocarbon Receptor ◽  
Ginkgo Biloba ◽  
Mammary Epithelial Cells ◽  
Ginkgo Biloba Extract ◽  
Mammary Epithelial ◽  
Aryl Hydrocarbon ◽  
Cyp1a1 Gene ◽  
Human Mammary Epithelial

The aryl hydrocarbon receptor (AhR) signaling pathway regulates the production of CYP1B1 and CYP1A1, which catalyze the bioactivation of various procarcinogens. In the present study, we investigated the effect of Ginkgo biloba extract and some of its chemical constituents on CYP1B1 and CYP1A1 gene expression and AhR activity in cultured MCF-10A human mammary epithelial cells. Treatment of MCF-10A cells with noncytotoxic concentrations of G. biloba extract (25–300 µg/mL for 24 or 48 h) increased CYP1B1 and CYP1A1 mRNA expression, which was accompanied by an increase in CYP1-mediated ethoxyresorufin O-dealkylation activity. The inductive effects of G. biloba extract were attenuated by an AhR antagonist (3′,4′-dimethoxyflavone). G. biloba extract (25–300 µg/mL) increased AhR-dependent reporter activity, as determined in MCF-10A cells transfected with an AhR-regulated luciferase reporter plasmid (pGudluc6.1). Bilobalide and ginkgolides A, B, C, and J were not responsible for the modulation of CYP1B1 and CYP1A1 gene expression or AhR activation by G. biloba extract. In contrast, quercetin increased CYP1B1 and CYP1A1 gene expression and activated AhR, whereas kaempferol and isorhamnetin suppressed constitutive CYP1B1 expression and antagonized AhR activation by benzo[a]pyrene. Overall, our findings provide an impetus for future investigations on the effect of G. biloba extract in CYP1-mediated chemical carcinogenesis.

Human mammary epithelial cells are sensitized to ionizing radiation by knockdown of caveolin-1

2010 ◽  
Vol 34 (8) ◽  
pp. S7-S7
Author(s):  
Nan Zhang ◽  
Jian Che ◽  
Zheng Wu ◽  
Yang Wang ◽  
Liwei Liu ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Ionizing Radiation ◽  
Mammary Epithelial Cells ◽  
Mammary Epithelial ◽  
Caveolin 1 ◽  
Human Mammary Epithelial Cells ◽  
Human Mammary Epithelial

Microphysiometry to Evaluate Real-Time Response of Mammary Epithelial Cells to IGF-I

2000 ◽  
Vol 28 (1-2) ◽  
pp. 209-211 ◽  
Author(s):  
Rose Marie Robinson ◽  
R. Michael Akers ◽  
Kimberly E. Forsten
Keyword(s):  
Epithelial Cells ◽  
Real Time ◽  
Mammary Epithelial Cells ◽  
Mammary Epithelial ◽  
Time Response ◽  
Igf I
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