Salt stress, among other abiotic stresses, has a high impact on crop yield. Salt tolerance is a multifactorial trait that involves the ability of cells to retain K ions, regulate reactive O species (ROS) production, and synthesise new molecules to cope with osmotic stress. In the present work, two different cultivars of Cucumis sativus L. (cv. Parys, sensitive; cv. Polan, tolerant) were selected based on their germination capabilities under 100 mM NaCl. The capacity of these two cultivars to tolerate salt stress was analysed using several different physiological and genetic approaches. K+ fluxes from roots, as an immediate response to salinity, showed the higher ability of cv. Polan to maintain K+ compared with cv. Parys, according to the expression level of inward rectifying potassium channel 1 (AKT1). ROS production was also investigated in both cultivars and a higher basal ROS level was observed in cv. Polan than in cv. Parys. Concurrently, an increased basal level of respiratory burst oxidase homologue F (RBOHF) gene was also found, as well as a strong induction of the ethylene responsive factor 109 (ERF109) transcription factor after salt treatment in cv. Polan. Our data suggest that roots’ ability to retain K+, a higher level of RBOHF and a strong induction of ERF109 should all be considered important components for salt tolerance in C. sativus.
Expression of jasmonic ethylene responsive factor gene in transgenic poplar tree leads to increased salt tolerance