scholarly journals Functional Analysis of the Asian Soybean Rust Resistance Pathway Mediated by Rpp2

2011 ◽  
Vol 24 (2) ◽  
pp. 194-206 ◽  
Author(s):  
Ajay K. Pandey ◽  
Chunling Yang ◽  
Chunquan Zhang ◽  
Michelle A. Graham ◽  
Heidi D. Horstman ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Candidate Genes ◽  
Leaf Tissue ◽  
Soybean Rust ◽  
Virus Induced Gene Silencing ◽  
Resistance Response ◽  
P450 Monooxygenase ◽  
Methyl Transferase ◽  
Asian Soybean Rust ◽  
Resistance Pathway

Asian soybean rust is an aggressive foliar disease caused by the obligate biotrophic fungus Phakopsora pachyrhizi. On susceptible plants, the pathogen penetrates and colonizes leaf tissue, resulting in the formation of necrotic lesions and the development of numerous uredinia. The soybean Rpp2 gene confers resistance to specific isolates of P. pachyrhizi. Rpp2-mediated resistance limits the growth of the pathogen and is characterized by the formation of reddish-brown lesions and few uredinia. Using virus-induced gene silencing, we screened 140 candidate genes to identify those that play a role in Rpp2 resistance toward P. pachyrhizi. Candidate genes included putative orthologs to known defense-signaling genes, transcription factors, and genes previously found to be upregulated during the Rpp2 resistance response. We identified 11 genes that compromised Rpp2-mediated resistance when silenced, including GmEDS1, GmNPR1, GmPAD4, GmPAL1, five predicted transcription factors, an O-methyl transferase, and a cytochrome P450 monooxygenase. Together, our results provide new insight into the signaling and biochemical pathways required for resistance against P. pachyrhizi.

scholarly journals Transcriptome analyses and virus induced gene silencing identify genes in the Rpp4-mediated Asian soybean rust resistance pathway

2013 ◽  
Vol 40 (10) ◽  
pp. 1029 ◽  
Author(s):  
Aguida M. A. P. Morales ◽  
Jamie A. O'Rourke ◽  
Martijn van de Mortel ◽  
Katherine T. Scheider ◽  
Timothy J. Bancroft ◽  
...  
Keyword(s):  
Gene Expression ◽  
Transcription Factors ◽  
Gene Silencing ◽  
Differentially Expressed ◽  
Soybean Rust ◽  
Phakopsora Pachyrhizi ◽  
R Gene ◽  
Virus Induced Gene Silencing ◽  
Asian Soybean Rust ◽  
Defence Responses

Rpp4 (Resistance to Phakopsora pachyrhizi 4) confers resistance to Phakopsora pachyrhizi Sydow, the causal agent of Asian soybean rust (ASR). By combining expression profiling and virus induced gene silencing (VIGS), we are developing a genetic framework for Rpp4-mediated resistance. We measured gene expression in mock-inoculated and P. pachyrhizi-infected leaves of resistant soybean accession PI459025B (Rpp4) and the susceptible cultivar (Williams 82) across a 12-day time course. Unexpectedly, two biphasic responses were identified. In the incompatible reaction, genes induced at 12 h after infection (hai) were not differentially expressed at 24 hai, but were induced at 72 hai. In contrast, genes repressed at 12 hai were not differentially expressed from 24 to 144 hai, but were repressed 216 hai and later. To differentiate between basal and resistance-gene (R-gene) mediated defence responses, we compared gene expression in Rpp4-silenced and empty vector-treated PI459025B plants 14 days after infection (dai) with P. pachyrhizi. This identified genes, including transcription factors, whose differential expression is dependent upon Rpp4. To identify differentially expressed genes conserved across multiple P. pachyrhizi resistance pathways, Rpp4 expression datasets were compared with microarray data previously generated for Rpp2 and Rpp3-mediated defence responses. Fourteen transcription factors common to all resistant and susceptible responses were identified, as well as fourteen transcription factors unique to R-gene-mediated resistance responses. These genes are targets for future P. pachyrhizi resistance research.

scholarly journals Identification and Analyses of Candidate Genes for Rpp4-Mediated Resistance to Asian Soybean Rust in Soybean

2009 ◽  
Vol 150 (1) ◽  
pp. 295-307 ◽  
Author(s):  
Jenelle D.F. Meyer ◽  
Danielle C.G. Silva ◽  
Chunling Yang ◽  
Kerry F. Pedley ◽  
Chunquan Zhang ◽  
...  
Keyword(s):  
Candidate Genes ◽  
Soybean Rust ◽  
Asian Soybean Rust

Asian Soybean Rust Sentinel Plots

2006 ◽  
Author(s):  
Ralph von Qualen ◽  
Xiao-Bing Yang
Keyword(s):  
Soybean Rust ◽  
Asian Soybean Rust

Asian Soybean Rust Sentinel Plots

2006 ◽  
Author(s):  
Ralph von Qualen ◽  
Xiao-Bing Yang
Keyword(s):  
Soybean Rust ◽  
Asian Soybean Rust

scholarly journals Functional examination of lncRNAs in allotetraploid Gossypium hirsutum

BMC Genomics ◽  
2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Luyao Wang ◽  
Jin Han ◽  
Kening Lu ◽  
Menglin Li ◽  
Mengtao Gao ◽  
...  
Keyword(s):  
Abiotic Stresses ◽  
Expression Patterns ◽  
Leaf Tissue ◽  
Evolutionary Model ◽  
Cotton Leaf ◽  
Functional Screening ◽  
Virus Induced Gene Silencing ◽  
Individual Gene ◽  
Cotton Species ◽  
Allotetraploid Cotton

Abstract Background An evolutionary model using diploid and allotetraploid cotton species identified 80 % of non-coding transcripts in allotetraploid cotton as being uniquely activated in comparison with its diploid ancestors. The function of the lncRNAs activated in allotetraploid cotton remain largely unknown. Results We employed transcriptome analysis to examine the relationship between the lncRNAs and mRNAs of protein coding genes (PCGs) in cotton leaf tissue under abiotic stresses. LncRNA expression was preferentially associated with that of the flanking PCGs. Selected highly-expressed lncRNA candidates (n = 111) were subjected to a functional screening pilot test in which virus-induced gene silencing was integrated with abiotic stress treatment. From this low-throughput screen, we obtained candidate lncRNAs relating to plant height and tolerance to drought and other abiotic stresses. Conclusions Low-throughput screen is an effective method to find functional lncRNA for further study. LncRNAs were more active in abiotic stresses than PCG expression, especially temperature stress. LncRNA XLOC107738 may take a cis-regulatory role in response to environmental stimuli. The degree to which lncRNAs are constitutively expressed may impact expression patterns and functions on the individual gene level rather than in genome-wide aggregate.

Identification and classification of Asian soybean rust using leaf-based hyperspectral reflectance

2021 ◽  
Vol 42 (11) ◽  
pp. 4177-4198
Author(s):  
Renato Herrig Furlanetto ◽  
Marcos Rafael Nanni ◽  
Monica Sayuri Mizuno ◽  
Luís Guilherme Teixeira Crusiol ◽  
Camila Rocco da Silva
Keyword(s):  
Soybean Rust ◽  
Hyperspectral Reflectance ◽  
Asian Soybean Rust

Registration of Asian Soybean Rust-Resistant Soybean Germplasm G01-PR16

2011 ◽  
Vol 5 (1) ◽  
pp. 118-122 ◽  
Author(s):  
H. Roger Boerma ◽  
Maria J. Monteros ◽  
Bo-Keun Ha ◽  
E. Dale Wood ◽  
Daniel V. Phillips ◽  
...  
Keyword(s):  
Soybean Rust ◽  
Soybean Germplasm ◽  
Asian Soybean Rust

scholarly journals Distinct Biphasic mRNA Changes in Response to Asian Soybean Rust Infection

2007 ◽  
Vol 20 (8) ◽  
pp. 887-899 ◽  
Author(s):  
Martijn van de Mortel ◽  
Justin C. Recknor ◽  
Michelle A. Graham ◽  
Dan Nettleton ◽  
Jaime D. Dittman ◽  
...  
Keyword(s):  
Gene Expression ◽  
Time Course ◽  
Expression Profiles ◽  
Fungal Growth ◽  
Initial Response ◽  
Soybean Rust ◽  
Specific Response ◽  
Susceptible Genotype ◽  
Rust Infection ◽  
Asian Soybean Rust

Asian soybean rust (ASR), caused by Phakopsora pachyrhizi, is now established in all major soybean-producing countries. Currently, there is little information about the molecular basis of ASR–soybean interactions, which will be needed to assist future efforts to develop effective resistance. Toward this end, abundance changes of soybean mRNAs were measured over a 7-day ASR infection time course in mock-inoculated and infected leaves of a soybean accession (PI230970) carrying the Rpp2 resistance gene and a susceptible genotype (Embrapa-48). The expression profiles of differentially expressed genes (ASR-infected compared with the mock-inoculated control) revealed a biphasic response to ASR in each genotype. Within the first 12 h after inoculation (hai), which corresponds to fungal germination and penetration of the epidermal cells, differential gene expression changes were evident in both genotypes. mRNA expression of these genes mostly returned to levels found in mock-inoculated plants by 24 hai. In the susceptible genotype, gene expression remained unaffected by rust infection until 96 hai, a time period when rapid fungal growth began. In contrast, gene expression in the resistant genotype diverged from the mock-inoculated control earlier, at 72 h, demonstrating that Rpp2-mediated defenses were initiated prior to this time. These data suggest that ASR initially induces a non-specific response that is transient or is suppressed when early steps in colonization are completed in both soybean genotypes. The race-specific resistance phenotype of Rpp2 is manifested in massive gene expression changes after the initial response prior to the onset of rapid fungal growth that occurs in the susceptible genotype.

scholarly journals A pigeonpea gene confers resistance to Asian soybean rust in soybean

2016 ◽  
Vol 34 (6) ◽  
pp. 661-665 ◽  
Author(s):  
Cintia G Kawashima ◽  
Gustavo Augusto Guimarães ◽  
Sônia Regina Nogueira ◽  
Dan MacLean ◽  
Doug R Cook ◽  
...  
Keyword(s):  
Soybean Rust ◽  
Asian Soybean Rust
Sign in / Sign up

Export Citation Format

Share Document