Mapping Determinants Within Cucumber Mosaic Virus and Its Satellite RNA for the Induction of Necrosis in Tomato Plants

1994 ◽  
Vol 7 (2) ◽  
pp. 189 ◽  
Author(s):  
David E. Sleat
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Satellite Rna ◽  
Tomato Plants

scholarly journals Analysis of Mechanisms Involved in the Cucumber mosaic virus Satellite RNA-mediated Transgenic Resistance in Tomato Plants

2004 ◽  
Vol 17 (1) ◽  
pp. 98-108 ◽  
Author(s):  
Fabrizio Cillo ◽  
Mariella M. Finetti-Sialer ◽  
Maria A. Papanice ◽  
Donato Gallitelli
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Rna Silencing ◽  
Resistance Mechanism ◽  
Degradation Process ◽  
Transgenic Tomato ◽  
Satellite Rna ◽  
Total Recovery ◽  
Tomato Plants ◽  
Free Strain

Transgenic tomato (Lycopersicon esculentum Mill. cv. UC82) plants expressing a benign variant of Cucumber mosaic virus satellite RNA (CMV Tfn-satRNA) were generated. The transformed plants did not produce symptoms when challenged with a satRNA-free strain of CMV (CMV-FL). The same plant lines initially were susceptible to necrosis elicited by a CMV strain supporting a necrogenic variant of satRNA (CMV-77), but a phenotype of total recovery from the necrosis was observed in the newly developing leaves. The features of the observed resistance were analyzed and are consistent with two different mechanisms of resistance. In transgenic plants inoculated with CMV-FL strain, the symptomless phenotype was correlated to the down-regulation of CMV by Tfn-satRNA, amplified from the transgene transcripts, as the first resistance mechanism. On the other hand, the delayed resistance to CMV-77 in transgenic tomato lines was mediated by a degradation process that targets satRNAs in a sequence-specific manner. Evidence is provided for a correlation between a reduced accumulation level of transgenic messenger Tfn-satRNA, the accumulation of small (approximately 23 nucleotides) RNAs with sequence homology to satRNAs, the progressively reduced accumulation of 77-satRNA in infected tissues, and the transition in infected plants from diseased to healthy. Thus, events leading to the degradation of satRNA sequences indicate a role for RNA silencing as the second mechanism determining resistance of transgenic tomato lines.

Transformed tomato plants express a satellite RNA of cucumber mosaic virus and produce lethal necrosis upon infection with viral RNA

1990 ◽  
Vol 170 (2) ◽  
pp. 548-555 ◽  
Author(s):  
P.B. McGarvey ◽  
J.M. Kaper ◽  
M.J. Avila-Rincon ◽  
L. Peña ◽  
J.R. Diaz-Ruiz
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Viral Rna ◽  
Satellite Rna ◽  
Tomato Plants

scholarly journals In Spite of Induced Multiple Defense Responses, Tomato Plants Infected with Cucumber mosaic virus and D Satellite RNA Succumb to Systemic Necrosis

2003 ◽  
Vol 16 (6) ◽  
pp. 467-476 ◽  
Author(s):  
Ping Xu ◽  
Elison B. Blancaflor ◽  
Marilyn J. Roossinck
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Expression Patterns ◽  
Defense Responses ◽  
Satellite Rna ◽  
Systemic Necrosis ◽  
Callose Deposition ◽  
Tomato Plants ◽  
Infected Tomato ◽  
Rna In Situ Hybridization

Cucumber mosaic virus (CMV) D satellite RNA (satRNA) attenuates the symptoms induced by CMV in most plants, but causes leaf epinasty and systemic necrosis in tomato plants, where programmed cell death (PCD) is involved. However, our understanding of the cellular and molecular responses to the infection of CMV D satRNA that result in this lethal disease remains limited. In this article, we show for the first time, by histochemical and molecular analysis, that multiple defense responses are specifically induced in CMV and D satRNA (CMV/D satRNA)-infected tomato plants but not in mock-inoculated or CMV-infected plants. These responses include callose deposition and hydrogen peroxide accumulation in infected plants. Furthermore, the transcription of several tomato defense-related genes (e.g., PR-1a1, PR-1b1, PR-2, and PR-10) were activated, and the expression of tomato PR-5 and some abiotic and biotic stress-responsive genes (e.g., catalase II and tomato analogs of Arabidopsis AtBI-1 and tobacco hsr203j) are enhanced. The activation and increase in expression of these genes is correlated with the appearance of leaf epinasty and the development of systemic necrosis in infected tomato plants, while increased expression of the hsr203j analog precedes the development of any disease symptoms. The spatial and temporal expression patterns of these genes as detected by RNA in situ hybridization point to the involvement of a complex developmental program that accompanies disease development resulting from CMV/D satRNA infection.

scholarly journals Transgenic Tomato Plants Expressing Satellite RNA Are Tolerant to Some Strains of Cucumber Mosaic Virus

1994 ◽  
Vol 119 (3) ◽  
pp. 642-647 ◽  
Author(s):  
P.B. McGarvey ◽  
M.S. Montasser ◽  
J.M. Kaper
Keyword(s):  
Transgenic Plants ◽  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Unit Length ◽  
Transgenic Tomato ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Satellite Rna ◽  
Tomato Plants ◽  
Transgenic Tomato Plants

Transgenic tomato plants (Lycopersicon esculentum Mill.) expressing cucumber mosaic virus (CMV) satellite RNA fused to a gene for β-glucuronidase were produced using Agrobacterium-mediated transformation. The R1 progeny of self-crossed R0 plants were challenge-inoculated with virion or RNA preparations of CMV or tomato aspermy virus (TAV). The transgenic plants challenged with CMV-1 showed mild disease symptoms in the first 2 weeks postchallenge followed by a decrease in symptoms, resulting in little difference between the transgenic and uninfected control group by the fourth week. Enzyme-linked immunosorbent assay results showed about a 10-fold decrease in virus accumulation in the transgenic plants compared to controls. Tolerance was evident only in plants that contained the recombinant insert and produced mature unit-length satellite RNA after CMV infection. Plants challenged with TAV showed no significant tolerance to virus-induced symptoms.

Cucumber mosaic virus-tolerant transgenic tomato plants expressing a satellite RNA

1992 ◽  
Vol 83-83 (6-7) ◽  
pp. 679-683 ◽  
Author(s):  
Y. Saito ◽  
T. Komari ◽  
C. Masuta1 ◽  
Y. Hayashi ◽  
T. Kumashiro ◽  
...  
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Transgenic Tomato ◽  
Satellite Rna ◽  
Tomato Plants ◽  
Transgenic Tomato Plants

scholarly journals A new satellite RNA is associated with natural infections of cucumber mosaic virus in succulent snap bean

2011 ◽  
Vol 157 (2) ◽  
pp. 375-377 ◽  
Author(s):  
Shahideh Nouri ◽  
Bryce W. Falk ◽  
Russell L. Groves
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Satellite Rna ◽  
Snap Bean
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