scholarly journals Tomato Cell Death Mediated By Complementary Plant Viral Satellite RNA Sequences

1998 ◽  
Vol 11 (12) ◽  
pp. 1214-1222 ◽  
Author(s):  
Michael E. Taliansky ◽  
Eugene V. Ryabov ◽  
David J. Robinson ◽  
Peter Palukaitis
Keyword(s):  
Cell Death ◽  
Secondary Structure ◽  
Potato Virus X ◽  
Computer Assisted ◽  
Satellite Rna ◽  
Rna Sequences ◽  
Virus Particles ◽  
Tomato Seedlings ◽  
Secondary Structure Analysis ◽  
Satellite Rnas

Cell death (necrosis) and severe yellowing (chlorosis) in tomato are induced by cucumber mosaic virus (CMV) supporting particular satellite RNAs. To determine whether CMV RNA sequences also are needed to induce necrosis or chlorosis, tomato seedlings were infected with the potato virus X vector expressing either a necrogenic or chlorosis-inducing satellite RNA of CMV. The infected plants did not develop chlorosis, although they did develop necrosis, but only when all or part of a 335-nucleotide necrogenic satellite RNA was expressed in the (-) polarity; i.e., the strand not packaged in virus particles. Computer-assisted secondary structure analysis suggests that the necrogenicity domain is an octanucleotide loop and adjacent base-paired stem of a thermodynamically stable hairpin structure.

scholarly journals Two Distinct Mechanisms of Transgenic Resistance Mediated by Groundnut Rosette Virus Satellite RNA Sequences

1998 ◽  
Vol 11 (5) ◽  
pp. 367-374 ◽  
Author(s):  
M. E. Taliansky ◽  
E. V. Ryabov ◽  
D. J. Robinson
Keyword(s):  
Nicotiana Benthamiana ◽  
Unit Length ◽  
Resistance Mechanism ◽  
Rna Replication ◽  
Potato Virus X ◽  
Resistance Mechanisms ◽  
Genomic Rna ◽  
Satellite Rna ◽  
Transgenic Resistance ◽  
Rna Sequences

Transformation of Nicotiana benthamiana with full-length sequences of a mild variant of the groundnut rosette virus (GRV) satellite RNA (sat-RNA) yielded plants that did not produce symptoms when inoculated with GRV and a virulent sat-RNA. Two different resistance mechanisms operated in different transformed lines. In the first, plants contained high levels of transcript RNA, and replication of both sat-RNA and GRV genomic RNA was inhibited. This mechanism is analogous to the down-regulation of GRV genomic and sat-RNA replication in infections containing the mild sat-RNA, and indeed infection of sat-RNA transgenic plants with GRV was shown to lead to liberation of unit-length sat-RNA from transgene transcripts. In the second resistance mechanism, plants contained low transcript RNA levels, and replication of sat-RNA but not of GRV genomic RNA was inhibited. These plants were also resistant to infection by potato virus X derivatives containing GRV sat-RNA sequences. This mechanism is an example of homology-dependent gene silencing or cosuppression. Resistant plants were also produced by transformation with sequences representing only the 5′ terminal one-third of the mild sat-RNA; the mechanism of resistance in these plants was of the cosuppression type.

scholarly journals Secondary Structure Analysis of Adenovirus Tripartite Leader

1989 ◽  
Vol 264 (18) ◽  
pp. 10679-10684
Author(s):  
Y Zhang ◽  
P J Dolph ◽  
R J Schneider
Keyword(s):  
Secondary Structure ◽  
Structure Analysis ◽  
Secondary Structure Analysis

Sequence of the Kluyveromyces lactis β-galactosidase: comparison with prokaryotic enzymes and secondary structure analysis

Gene ◽  
1992 ◽  
Vol 118 (1) ◽  
pp. 55-63 ◽  
Author(s):  
Olivier Poch ◽  
Hervé L'Hôte ◽  
Vincent Dallery ◽  
Françoise Debeaux ◽  
Reinhard Fleer ◽  
...  
Keyword(s):  
Secondary Structure ◽  
Structure Analysis ◽  
Kluyveromyces Lactis ◽  
Secondary Structure Analysis

Partial characterization of an RNA component that copurifies with Saccharomyces cerevisiae RNase P

1989 ◽  
Vol 9 (6) ◽  
pp. 2536-2543
Author(s):  
J Y Lee ◽  
D R Engelke
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Secondary Structure ◽  
Schizosaccharomyces Pombe ◽  
Rnase P ◽  
Partial Characterization ◽  
Rna Sequences ◽  
Extensive Sequence ◽  
Sequence Similarities

Saccharomyces cerevisiae cellular RNase P is composed of both protein and RNA components that are essential for activity. The isolated holoenzyme contains a highly structured RNA of 369 nucleotides that has extensive sequence similarities to the 286-nucleotide RNA associated with Schizosaccharomyces pombe RNase P but bears little resemblance to the analogous RNA sequences in procaryotes or S. cerevisiae mitochondria. Even so, the predicted secondary structure of S. cerevisiae RNA is strikingly similar to the bacterial phylogenetic consensus rather than to previously predicted structures of other eucaryotic RNase P RNAs.

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