scholarly journals cDNA Macroarray Analysis of Gene Expression in Ineffective Nodules Induced on the Lotus japonicus sen1 Mutant

2004 ◽  
Vol 17 (11) ◽  
pp. 1223-1233 ◽  
Author(s):  
Norio Suganuma ◽  
Atsuko Yamamoto ◽  
Ai Itou ◽  
Tsuneo Hakoyama ◽  
Mari Banba ◽  
...  
Keyword(s):  
Gene Expression ◽  
Nitrogen Fixation ◽  
Nitrogenase Activity ◽  
Cysteine Proteinase ◽  
Lotus Japonicus ◽  
Vascular Bundles ◽  
Cdna Macroarray ◽  
Plant Genes ◽  
Macroarray Analysis ◽  
Mutant Forms

The Lotus japonicus sen1 mutant forms ineffective nodules in which development is arrested at the stage of bacterial differentiation into nitrogen-fixing bacteroids. Here, we used cDNA macroarray systems to compare gene expression in ineffective nodules induced on the sen1 mutant with gene expression in wild-type nodules, in order to identify the host plant genes that are involved in nitrogen fixation. Macroarray analysis coupled with Northern blot analysis revealed that the expression of 18 genes was significantly enhanced in ineffective sen1 nodules, whereas the expression of 30 genes was repressed. Many of the enhanced genes encoded hydrolase enzymes, such as cysteine proteinase and asparaginase, that might function in the early senescence of sen1 nodules. By contrast, the repressed genes encoded nodulins, enzymes that are involved in carbon and nitrogen metabolism, membrane transporters, enzymes involved in phytohormone metabolism and secondary metabolism, and regulatory proteins. These proteins might have a role in the establishment of nitrogen fixation. In addition, we discovered two novel genes that encoded glutamate-rich proteins and were localized in the vascular bundles of the nodules. The expression of these genes was repressed in the ineffective nodules, which had lower levels of nitrogenase activity.

scholarly journals Absence of Symbiotic Leghemoglobins Alters Bacteroid and Plant Cell Differentiation During Development of Lotus japonicus Root Nodules

2009 ◽  
Vol 22 (7) ◽  
pp. 800-808 ◽  
Author(s):  
Thomas Ott ◽  
John Sullivan ◽  
Euan K. James ◽  
Emmanouil Flemetakis ◽  
Catrin Günther ◽  
...  
Keyword(s):  
Gene Expression ◽  
Nitrogen Fixation ◽  
Cell Differentiation ◽  
Root Nodules ◽  
Lotus Japonicus ◽  
Nodule Development ◽  
Primary And Secondary Metabolism ◽  
Bacterial Differentiation ◽  
Plant Genes ◽  
Bacterial Genes

During development of legume root nodules, rhizobia and their host plant cells undergo profound differentiation, which is underpinned by massive changes in gene expression in both symbiotic partners. Oxygen concentrations in infected and surrounding uninfected cells drop precipitously during nodule development. To assess what effects this has on plant and bacterial cell differentiation and gene expression, we used a leghemoglobin-RNA-interference (LbRNAi) line of Lotus japonicus, which is devoid of leghemoglobins and has elevated levels of free-oxygen in its nodules. Bacteroids in LbRNAi nodules showed altered ultrastructure indicating changes in bacterial differentiation. Transcript analysis of 189 plant and 192 bacterial genes uncovered many genes in both the plant and bacteria that were differentially regulated during nodulation of LbRNAi plants compared with the wild type (containing Lb and able to fix nitrogen). These included fix and nif genes of the bacteria, which are involved in microaerobic respiration and nitrogen fixation, respectively, and plant genes involved in primary and secondary metabolism. Metabolite analysis revealed decreased levels of many amino acids in nodules of LbRNAi plants, consistent with the defect in symbiotic nitrogen fixation of this line.

scholarly journals Identification, using cDNA macroarray analysis, of distinct gene expression profiles associated with pathological and virological features of hepatocellular carcinoma

Oncogene ◽  
2002 ◽  
Vol 21 (18) ◽  
pp. 2926-2937 ◽  
Author(s):  
Oona Delpuech ◽  
Jean-Baptiste Trabut ◽  
Françoise Carnot ◽  
Jean Feuillard ◽  
Christian Brechot ◽  
...  
Keyword(s):  
Gene Expression ◽  
Hepatocellular Carcinoma ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Cdna Macroarray ◽  
Distinct Gene ◽  
Macroarray Analysis

Gene expression profiling of adrenal cortical tumors by cDNA macroarray analysis. Results of a preliminary study

2006 ◽  
Vol 60 (4) ◽  
pp. 186-190 ◽  
Author(s):  
C.P. Lombardi ◽  
M. Raffaelli ◽  
G. Pani ◽  
A. Maffione ◽  
P. Princi ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Expression Profiling ◽  
Expression Profiling ◽  
Cdna Macroarray ◽  
Preliminary Study ◽  
Macroarray Analysis

scholarly journals Anabaena sp. Strain PCC 7120 Gene devH Is Required for Synthesis of the Heterocyst Glycolipid Layer

2005 ◽  
Vol 187 (7) ◽  
pp. 2326-2331 ◽  
Author(s):  
Martha E. Ramírez ◽  
Pratibha B. Hebbar ◽  
Ruanbao Zhou ◽  
C. Peter Wolk ◽  
Stephanie E. Curtis
Keyword(s):  
Nitrogen Fixation ◽  
Nitrogenase Activity ◽  
Regulatory Protein ◽  
Protein A ◽  
Filamentous Cyanobacterium ◽  
Fixed Nitrogen ◽  
Ultrastructural Studies ◽  
Anabaena Sp ◽  
Pcc 7120 ◽  
Mutant Forms

ABSTRACT In response to deprivation for fixed nitrogen, the filamentous cyanobacterium Anabaena sp. strain PCC 7120 provides a microoxic intracellular environment for nitrogen fixation through the differentiation of semiregularly spaced vegetative cells into specialized cells called heterocysts. The devH gene is induced during heterocyst development and encodes a product with characteristics of a trans-acting regulatory protein. A devH mutant forms morphologically distinguishable heterocysts but is Fox−, incapable of nitrogen fixation in the presence of oxygen. We demonstrate that rearrangements of nitrogen fixation genes take place normally in the devH mutant and that it is Fix+, i.e., has nitrogenase activity under anoxic conditions. The Fox− phenotype was shown by ultrastructural studies to be associated with the absence of the glycolipid layer of the heterocyst envelope. The expression of glycolipid biosynthetic genes in the mutant is greatly reduced, and heterocyst glycolipids are undetectable.

scholarly journals A Dicarboxylate Transporter, LjALMT4, Mainly Expressed in Nodules of Lotus japonicus

2016 ◽  
Vol 29 (7) ◽  
pp. 584-592 ◽  
Author(s):  
Kojiro Takanashi ◽  
Takayuki Sasaki ◽  
Tomohiro Kan ◽  
Yuka Saida ◽  
Akifumi Sugiyama ◽  
...  
Keyword(s):  
Nitrogen Fixation ◽  
Symbiotic Nitrogen Fixation ◽  
Lotus Japonicus ◽  
Specific Gene ◽  
Vascular Bundles ◽  
Transport Activity ◽  
Organ Specific ◽  
Infected Cells ◽  
Photosynthetic Products ◽  
Strong Candidate

Legume plants can establish symbiosis with soil bacteria called rhizobia to obtain nitrogen as a nutrient directly from atmospheric N2 via symbiotic nitrogen fixation. Legumes and rhizobia form nodules, symbiotic organs in which fixed-nitrogen and photosynthetic products are exchanged between rhizobia and plant cells. The photosynthetic products supplied to rhizobia are thought to be dicarboxylates but little is known about the movement of dicarboxylates in the nodules. In terms of dicarboxylate transporters, an aluminum-activated malate transporter (ALMT) family is a strong candidate responsible for the membrane transport of carboxylates in nodules. Among the seven ALMT genes in the Lotus japonicus genome, only one, LjALMT4, shows a high expression in the nodules. LjALMT4 showed transport activity in a Xenopus oocyte system, with LjALMT4 mediating the efflux of dicarboxylates including malate, succinate, and fumarate, but not tricarboxylates such as citrate. LjALMT4 also mediated the influx of several inorganic anions. Organ-specific gene expression analysis showed LjALMT4 mRNA mainly in the parenchyma cells of nodule vascular bundles. These results suggest that LjALMT4 may not be involved in the direct supply of dicarboxylates to rhizobia in infected cells but is responsible for supplying malate as well as several anions necessary for symbiotic nitrogen fixation, via nodule vasculatures.

scholarly journals Azorhizobium caulinodans PIIand GlnK Proteins Control Nitrogen Fixation and Ammonia Assimilation

1999 ◽  
Vol 181 (8) ◽  
pp. 2655-2658 ◽  
Author(s):  
Nathalie Michel-Reydellet ◽  
P. Alexandre Kaminski
Keyword(s):  
Gene Expression ◽  
Nitrogen Fixation ◽  
Nitrogenase Activity ◽  
Ammonia Assimilation ◽  
Ammonium Excretion ◽  
Azorhizobium Caulinodans ◽  
Wild Type ◽  
Free Living ◽  
Living State ◽  
High Level

ABSTRACT We herein report that Azorhizobium caulinodansPII and GlnK are not necessary for glutamine synthetase (GS) adenylylation whereas both proteins are required for complete GS deadenylylation. The disruption of both glnB andglnK resulted in a high level of GS adenylylation under the condition of nitrogen fixation, leading to ammonium excretion in the free-living state. PII and GlnK also controllednif gene expression because NifA activated nifHtranscription and nitrogenase activity was derepressed in glnB glnK double mutants, but not in wild-type bacteria, grown in the presence of ammonia.

scholarly journals Transcriptomic Changes in Medicago truncatula and Lotus japonicus Root Nodules during Drought Stress

2019 ◽  
Vol 20 (5) ◽  
pp. 1204 ◽  
Author(s):  
Izabela Sańko-Sawczenko ◽  
Barbara Łotocka ◽  
Jakub Mielecki ◽  
Hanna Rekosz-Burlaga ◽  
Weronika Czarnocka
Keyword(s):  
Gene Expression ◽  
Medicago Truncatula ◽  
Sinorhizobium Meliloti ◽  
Nitrogenase Activity ◽  
Root Nodules ◽  
Lotus Japonicus ◽  
Global Gene Expression ◽  
Water Shortage ◽  
Mesorhizobium Loti ◽  
Yield Production

Drought is one of the major environmental factors limiting biomass and seed yield production in agriculture. In this research, we focused on plants from the Fabaceae family, which has a unique ability for the establishment of symbiosis with nitrogen-fixing bacteria, and are relatively susceptible to water limitation. We have presented the changes in nitrogenase activity and global gene expression occurring in Medicago truncatula and Lotus japonicus root nodules during water deficit. Our results proved a decrease in the efficiency of nitrogen fixation, as well as extensive changes in plant and bacterial transcriptomes, shortly after watering cessation. We showed for the first time that not only symbiotic plant components but also Sinorhizobium meliloti and Mesorhizobium loti bacteria residing in the root nodules of M. truncatula and L. japonicus, respectively, adjust their gene expression in response to water shortage. Although our results demonstrated that both M. truncatula and L. japonicus root nodules were susceptible to water deprivation, they indicated significant differences in plant and bacterial response to drought between the tested species, which might be related to the various types of root nodules formed by these species.

cDNA macroarray analysis of gene expression changes in rat brain after a single administration of a 2-aminoadamantane derivative

2005 ◽  
Vol 39 (2) ◽  
pp. 244-252 ◽  
Author(s):  
Yu. V. Vakhitova ◽  
R. S. Yamidanov ◽  
V. A. Vakhitov ◽  
S. B. Seredenin
Keyword(s):  
Gene Expression ◽  
Rat Brain ◽  
Single Administration ◽  
Cdna Macroarray ◽  
Aminoadamantane Derivative ◽  
Macroarray Analysis

cDNA macroarray analysis of gene expression in synoviocytes stimulated with TNFα

FEBS Letters ◽  
2002 ◽  
Vol 517 (1-3) ◽  
pp. 121-128 ◽  
Author(s):  
Tomoyasu Sugiyama ◽  
Shizuko Ishii ◽  
Jun-ichi Yamamoto ◽  
Ryotaro Irie ◽  
Kaoru Saito ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cdna Macroarray ◽  
Macroarray Analysis
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