Occurrence of Cymbidium Mosaic Virus and Odontoglossum Ring-spot Virus in Korea.

Plant Disease ◽  
1995 ◽  
Vol 79 ◽  
pp. 321 ◽  
Author(s):  
Ki Hyun Ryu
2015 ◽  
Vol 11 (1) ◽  
Author(s):  
Silvina Edith Cánovas ◽  
María Celeste Ballari ◽  
Claudia Fernanda Nome

2013 ◽  
Vol 832 ◽  
pp. 113-117 ◽  
Author(s):  
Shahrul A.B. Ariffin ◽  
Tijjani Adam ◽  
U. Hashim ◽  
S. Faridah Sfaridah ◽  
Ishak Zamri ◽  
...  

The plant disease such as Cucumber Mosaic Virus (CMV) and Papaya Ring Spot Virus (PRSV) is a most dangerous disease that can decrease productivity and quality of the vegetable and fruit. Besides that, its also can destroy and kill those plant in long term when infected and to tackle this problem at early stages, the nanowire based biosensor application is a most reliable sensor nowadays because of advantages towards detecting biological molecule especially plant diseases.In order to dealing with tiny form of molecules such as virus is very difficult and due to the nanostructure uniqueness such as nanowire, it can be done by undergo formation of nanowire process.Result will be elaborated about how nanowire working environment in order to detecting those virus.


1980 ◽  
Vol 58 (16) ◽  
pp. 1747-1754 ◽  
Author(s):  
H. F. Dias ◽  
W. R. Allen

Purified preparations of peach rosette mosaic virus (PRMV), were shown by rate-zonal centrifugation in sucrose and equilibrium centrifugation in CsCl to be composed of two nucleoprotein components with buoyant densities of 1.47 (middle) and 1.51 (bottom) g/cm3. The virus contains two RNA species with molecular weights of 2.5 × 106 (RNA 1) and 2.2 × I06 (RNA 2), and a single protein subunit with a molecular weight of 57 000. RNA 1 and RNA 2 reside separately in components B and M, respectively. Both RNAs are required for infection thus indicating that the virus has a divided genome. The nucleotide composition of both RNAs is similar except for cytidilic acid. The hyperchromic profile for the M component is broader than that of B and the Tm value is higher (for M Tm = 55 °C; for B Tm = 48 °C). Particle disruption and release of RNA progresses slowly over the absorbance–temperature transition. Only half of the particles were dissociated at the Tm value. Freezing dissociates most of M component into RNA 2 and protein but had no effect on the B component. Sodium chloride protected the M particles from low temperature disruption. The data support the conclusion that PRMV is a nepovirus with particular properties of the tomato ring-spot virus (TomRSV) subgroup.


Lankesteriana ◽  
2015 ◽  
Vol 7 (1-2) ◽  
Author(s):  
German Rivera-Coto ◽  
Gilberto Corrales-Moreira

Orchids from private collections, nurseries and wild areas, located in different ecological regions of Costa Rica, were studied in order to know the pests and diseases affecting them. 16 insect and two mite genera were identified as pests on different orchids and 28 pathogens were found as causal agents of infec- tious diseases. The most frequent pests were: Tenthecoris orchidearum, Stethobaris sp., Xylosandrus com- pactus, Pseudococcus longispinus, Diaspis boisduvalii and Tenuipalpus pacificus. The main diseases found were caused by: Phytophthora spp., Pythium spp., Fusariun oxysporum, Fusarium spp., Sclerotium rolfsii, Colletotrichum gloeosporioides, Sphenospora spp., Uredo spp., Cymbidium Mosaic virus and Odontoglossum Ring Spot Virus. This results demonstrate the diversity of organisms producing sanitary problems on orchids in Costa Rica, and suggests the inclusion of pest and diseases management in strate- gies for orchid conservation.


2012 ◽  
Vol 6 (1) ◽  
pp. 26-32
Author(s):  
Houda Z. Kawas

108 sample Collected from the fields of farmers in the areas of apple cultivation in the south of Syria during the years 1998-2007, and the most important symptoms associated with infection were recorded, results of the biometric tests (mechanical inoculation on indicator plant) and examination by electron microscope and serological tests (ELISA) using antisera of Apple mosaic virus, Apple chlorotic leaf spot virus, Tomato ring spot virus , Tomato spotted wilt virus, Tobacco ring spot virus , Tomato black ring virus and Arabis mosaic virus to the spread of a virus infection of Apple chlorotic leaf spot virus (ACLSV) by 24%, Apple mosaic virus (ApMV) by 26.9% and to register cases Tomato ring spot virus (TomRSV) by 13% and Tobacco ring spot virus (TRSV) by %14.8, Tomato black ring virus (TBRV) rate of % 12.03 and Arabis mosaic virus (ArMV) 2.43% for the first time on apples in Syria, and the likelihood of several viral and viroid diseases, that we need to reassess the health situation in view of the importance of maintaining the cultivation of apples and recommended program documentation for the production of propagation of disease-free, with proposal to use molecular methods to detect and identify viral diseases causes and strains prevalent in Syria.


Author(s):  
M. G. F. O. Soares ◽  
J. A. Soares ◽  
M. A. Cezar ◽  
T. A. L. Cardoso ◽  
J. A. A. Lima

<p>Por constituírem uma importante fonte de alimento,<strong> </strong>plantações de melancia e abóbora são comumente cultivadas no sertão paraibano, porém pouco se sabe sobre a ocorrência de patógenos causadores de doenças, os quais limitam a sua produtividade e renda aos produtores. Visando obter informações sobre a ocorrência dos patógenos virais e fúngicos em cultivos de abóbora e melancia situados em municípios produtores no sertão da Paraíba, amostras coletadas com sintomas de mosaico e deformação foliar, típicos de doenças virais foram analisadas pela técnica sorológica “enzime linked immuno sorbentassay” (Elisa) indireto para <em>Papaya ring spot virus, </em>type watermelon (PRSV-W), <em>Watermelon mosaic virus </em>(WMV), <em>Zucchini yellow mosaic virus </em>(ZYMV) e <em>Cucumber mosaic virus </em>(CMV). O teste de dupla difusão em Agar foi utilizado para verificar a presença de <em>Squash mosaic virus </em>(SqMV). Em contrapartida, amostras coletadas com sintomas de doenças fúngicas foram analisadas pelo isolamento do patógeno e visualização de suas características morfológicas em microscópio óptico. Em abóbora, houve prevalência dos vírus ZYMV e PRSV-W em infecções simples e mistas, e maior incidência dos fungos <em>Cladosporium</em> spp., e <em>Alternaria</em> spp. Em melancia detectou-se<em> </em>infecção simples e mistas das espécies PRSV-W, WMV e ZYMV, e maior freqüência de <em>Fusarium</em> spp. e <em>Alternaria</em> spp.. Não foram detectados os vírus CMV e SqMV. Os resultados obtidos revelam a ocorrência de vários patógenos fúngicos e viróticos em cultivos de abóbora e melancia situados no sertão da Paraíba e ressaltam a importância da utilização de estratégias de manejo que reduzem os danos ocasionados por esses patógenos.</p><p align="center"><strong><em>Occurrence of pathogens in watermelon and pumpkin crops in the State of Paraiba</em></strong></p><p><strong>Abstract:</strong> By constitute an important food source, watermelon and pumpkin plantations are commonly grown on Paraíba<strong> </strong>backlands, but little is known about the occurrence of disease-causing pathogens, which limit their productivity and income to producers. With objective to get information about the viral and fungal pathogens occurrence in pumpkin and watermelon crops in producing counties located in the backlands of Paraiba, samples with symptoms of mosaic and leaf distortion, typical of viral etiology of disease were collected and analyzed by "Enzyme linked immune sorbentassay" (Elisa) Indirect for species <em>Papaya ring spot virus</em>, type watermelon (PRSV-W), <em>Watermelon mosaic virus </em>(WMV), <em>Zucchini yellow mosaic virus</em> ( ZYMV) and <em>Cucumber mosaic virus</em> (CMV). The double diffusion test in Agar was used to verify the presence of <em>Squash mosaic virus</em> (SqMV). In contrast, samples collected with fungal disease symptoms were analyzed based on the isolation of the pathogen and viewing their morphological characteristics under an optical microscope. In pumpkin, there was prevalence of ZYMV and PRSV-W in single and mixed infections, and higher incidence of <em>Cladosporium</em> spp. and <em>Alternaria</em> spp. On the other hand, in watermelon was detected single and mixed infections of PRSV-W, WMV and ZYMV species, and higher frequency of <em>Fusarium </em>spp. and <em>Alternaria</em> spp.. Were not detected the virus CMV and SqMV. The results show the occurrence of various fungal and viral pathogens in pumpkin and watermelon crops located in the backlands of Paraiba and emphasize the importance of using management strategies that reduce the damage caused by these pathogens.</p>


2013 ◽  
Vol 31 (1) ◽  
pp. 45-49
Author(s):  
Antonio Ismael Inácio Cardoso ◽  
Marcelo A Pavan

O objetivo do trabalho foi verificar o efeito protetor de um isolado atenuado de "Papaya ring spot virus - type Watermelon" (PRSV-W) e um de "Zucchini yellow mosaic vírus" (ZYMV) em plantas de abobrinha-de-moita, cultivar Caserta, visando a produção de sementes. Foram testados cinco tratamentos, com seis repetições, no delineamento experimental em blocos ao acaso. Os tratamentos consistiram em: 1) premunização com PRSV-W; 2) premunização com ZYMV; 3) premunização dupla (PRSV-W e ZYMV); 4) testemunha com plantas submetidas à infecção natural; 5) plantas isentas de vírus. Obteve-se maior número de frutos em plantas sem vírus, em comparação aos demais tratamentos, enquanto que a premunização com PRSV-W foi superior à testemunha com infecção natural. No tratamento com premunização dupla foram obtidos frutos com menor massa média que as plantas sem vírus, não havendo diferença entre os outros tratamentos. Para produção de sementes por planta, todos os tratamentos com premunização e a testemunha não diferiram entre si, porém produziram menos que as plantas isentas de vírus. Para a qualidade (germinação e vigor) das sementes não foram verificadas diferenças entre os tratamentos.


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