Characterization of the single protein and two nucleic acids of peach rosette mosaic virus

Purified preparations of peach rosette mosaic virus (PRMV), were shown by rate-zonal centrifugation in sucrose and equilibrium centrifugation in CsCl to be composed of two nucleoprotein components with buoyant densities of 1.47 (middle) and 1.51 (bottom) g/cm3. The virus contains two RNA species with molecular weights of 2.5 × 106 (RNA 1) and 2.2 × I06 (RNA 2), and a single protein subunit with a molecular weight of 57 000. RNA 1 and RNA 2 reside separately in components B and M, respectively. Both RNAs are required for infection thus indicating that the virus has a divided genome. The nucleotide composition of both RNAs is similar except for cytidilic acid. The hyperchromic profile for the M component is broader than that of B and the Tm value is higher (for M Tm = 55 °C; for B Tm = 48 °C). Particle disruption and release of RNA progresses slowly over the absorbance–temperature transition. Only half of the particles were dissociated at the Tm value. Freezing dissociates most of M component into RNA 2 and protein but had no effect on the B component. Sodium chloride protected the M particles from low temperature disruption. The data support the conclusion that PRMV is a nepovirus with particular properties of the tomato ring-spot virus (TomRSV) subgroup.

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Properties of the single protein and two nucleic acids of tomato ring-spot virus

Canadian Journal of Botany ◽

10.1139/b77-120 ◽

1977 ◽

Vol 55 (8) ◽

pp. 1028-1037 ◽

Cited By ~ 8

Author(s):

Wayne R. Allen ◽

H. F. Dias

Keyword(s):

Nucleic Acid ◽

Nucleic Acids ◽

Gel Electrophoresis ◽

Average Molecular Weight ◽

Virus Genome ◽

Protein Subunit ◽

Spot Virus ◽

Single Protein ◽

Middle Component ◽

Ring Spot

Purified preparations of several isolates of tomato ring-spot virus were shown by rate-zonal centrifugation in sucrose and equilibrium centrifugation in CsCl to be composed of two individual nucleoprotein components. Acrylamide-gel electrophoresis showed that the lighter (middle) component contained a nucleic acid (RNA 2) that was distinct from the species (RNA 1) contained in the heavier (bottom) component. The bottom was more infectious than the middle component and infectivity was enhanced by mixing the components, indicating that the virus genome is divided between component types. Similar results were obtained from infectivity tests on the two nucleic acids. The nucleic acid contents of the middle and bottom components were about 40 and 41%, respectively. The average molecular weights of RNA 2 and RNA 1 from three virus isolates, as determined by acrylamide-gel electrophoresis, were 2.5 and 2.6 × 106, respectively. Molecular complexing between the RNA species during electrophoresis was prevented with the use of formamide. The single protein subunit from the same three isolates had an average molecular weight of about 58 000. Serological comparisons of five tomato ring-spot isolates associated with diseases of fruit trees and grapevines indicated that only the grape yellow vein strain was antigenically distinct. These and other properties indicate that this virus is similar to other members of the nepovirus group.

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Molecular characterization of ilarvirus Prunus necrotic ring spot virus (PNRSV) and Prune dwarf virus (PDV) in Spanish autochthonous almond cultivars

Acta Horticulturae ◽

10.17660/actahortic.2018.1219.53 ◽

2018 ◽

pp. 353-356

Author(s):

M. Rubio ◽

P. Martínez-Gómez ◽

V. Pallás ◽

F. Dicenta ◽

J.A. Sánchez-Navarro

Keyword(s):

Molecular Characterization ◽

Dwarf Virus ◽

Spot Virus ◽

Prune Dwarf Virus ◽

Ring Spot

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Occurrence of Cymbidium Mosaic Virus and Odontoglossum Ring-spot Virus in Korea.

Plant Disease ◽

10.1094/pd-79-0321d ◽

1995 ◽

Vol 79 ◽

pp. 321 ◽

Cited By ~ 7

Author(s):

Ki Hyun Ryu

Keyword(s):

Mosaic Virus ◽

Spot Virus ◽

Cymbidium Mosaic Virus ◽

Ring Spot

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Plant Diseases Detection Using Nanowire as Biosensor Transducer

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.832.113 ◽

2013 ◽

Vol 832 ◽

pp. 113-117 ◽

Cited By ~ 21

Author(s):

Shahrul A.B. Ariffin ◽

Tijjani Adam ◽

U. Hashim ◽

S. Faridah Sfaridah ◽

Ishak Zamri ◽

...

Keyword(s):

Mosaic Virus ◽

Plant Disease ◽

Plant Diseases ◽

Working Environment ◽

Spot Virus ◽

Biosensor Application ◽

Papaya Ring Spot Virus ◽

Ring Spot

The plant disease such as Cucumber Mosaic Virus (CMV) and Papaya Ring Spot Virus (PRSV) is a most dangerous disease that can decrease productivity and quality of the vegetable and fruit. Besides that, its also can destroy and kill those plant in long term when infected and to tackle this problem at early stages, the nanowire based biosensor application is a most reliable sensor nowadays because of advantages towards detecting biological molecule especially plant diseases.In order to dealing with tiny form of molecules such as virus is very difficult and due to the nanostructure uniqueness such as nanowire, it can be done by undergo formation of nanowire process.Result will be elaborated about how nanowire working environment in order to detecting those virus.

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Isolation and spectral characterization of phycobiliproteins

Biochemical Journal ◽

10.1042/bj1160161 ◽

1970 ◽

Vol 116 (2) ◽

pp. 161-169 ◽

Cited By ~ 119

Author(s):

F. W. J. Teale ◽

R. E. Dale

Keyword(s):

Energy Transfer ◽

Ionic Strength ◽

Aqueous Medium ◽

Fluorescence Emission ◽

Protein Subunit ◽

Fluorescence Excitation ◽

Different Types ◽

Single Protein ◽

Polarization Spectra

Several phycobiliproteins were prepared chromatographically pure and their absorption, fluorescence-emission, fluorescence-excitation and fluorescence-excitation polarization spectra determined. Changes in these spectra with ionic strength of the aqueous medium and chromoprotein concentration were interpreted in terms of interchromophore energy transfer and protein subunit equilibria. The complexity of the polarization spectra confirms the presence of different types of chromophore, designated sensitizing (‘s’) and fluorescing (‘f’), in a single protein.

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