scholarly journals Genetic Relationship of Stripe Rust Resistance Genes Yr34 and Yr48 in Wheat and Identification of Linked KASP Markers

Plant Disease ◽  
2018 ◽  
Vol 102 (2) ◽  
pp. 413-420 ◽  
Author(s):  
N. Qureshi ◽  
H. S. Bariana ◽  
P. Zhang ◽  
R. McIntosh ◽  
U. K. Bansal ◽  
...  
Keyword(s):  
Resistance Gene ◽  
Stripe Rust ◽  
Rust Resistance ◽  
Puccinia Striiformis ◽  
Rust Resistance Gene ◽  
Stripe Rust Resistance ◽  
Adult Plant ◽  
Wheat Genotypes ◽  
Stripe Rust Resistance Gene ◽  
Australian Continent

The Australian continent was free from wheat stripe rust caused by Puccinia striiformis f. sp. tritici until exotic incursions occurred in 1979 and 2002. The 2002 incursion enabled the identification of a new stripe rust resistance gene (Yr34) in the advanced breeding line WAWHT2046. In this study, we developed and validated markers closely linked with Yr34, which is located in the distal region in the long arm of chromosome 5A. Four kompetitive allele-specific polymerase chain reaction (KASP) and three sequence-tagged site (STS) markers derived from the International Wheat Genome Sequencing Consortium RefSeq v1.0 scaffold-77836 cosegregated with Yr34. Markers sun711, sun712, sun725, sunKASP_109, and sunKASP_112 were shown to be suitable for marker-assisted selection in a validation panel of 71 Australian spring wheat genotypes, with the exception of cultivar Orion that carried the Yr34-linked alleles for sunKASP_109 and sunKASP_112. Markers previously reported to be linked with adult plant stripe rust resistance gene Yr48 also cosegregated with Yr34. Wheat genotypes carrying Yr34 and Yr48 produced identical haplotypes for the Yr34-linked markers identified in this study and those previously reported to be linked with Yr48. Phenotypic testing of genotypes carrying Yr34 and Yr48 showed that both genes conferred similar seedling responses to pre-2002 and post-2002 P. striiformis f. sp. tritici pathotypes. Further testing of 600 F2 plants from a cross between WAWHT2046 and RIL143 (Yr48) with P. striiformis f. sp. tritici pathotype 134 E16A+Yr17+Yr27+ failed to reveal any susceptible segregants. Our results strongly suggest that Yr34 and Yr48 are the same gene, and that Yr48 should be considered a synonym of Yr34.

scholarly journals Genetic and Molecular Mapping of Stripe Rust Resistance Gene in Wheat–Psathyrostachys huashanica Translocation Line H9020-1-6-8-3

Plant Disease ◽  
2012 ◽  
Vol 96 (10) ◽  
pp. 1482-1487 ◽  
Author(s):  
Qiang Li ◽  
Jing Huang ◽  
Lu Hou ◽  
Pei Liu ◽  
Jinxue Jing ◽  
...  
Keyword(s):  
Resistance Gene ◽  
Stripe Rust ◽  
Rust Resistance ◽  
Puccinia Striiformis ◽  
Durable Resistance ◽  
Rust Resistance Gene ◽  
Stripe Rust Resistance ◽  
Translocation Line ◽  
Psathyrostachys Huashanica ◽  
Stripe Rust Resistance Gene

Stripe rust, caused by Puccinia striiformis f. sp. tritici, is one of the most important diseases of wheat worldwide. The best strategy to control stripe rust is to grow resistant cultivars, but only a few effective genes are available. The wheat accession H9020-1-6-8-3 is a translocation line previously developed from interspecific hybridization between wheat genotype 7182 and Psathyrostachys huashanica, and is resistant to most Chinese Puccinia striiformis f. sp. tritici races. To identify the resistance genes in the translocation line, H9020-1-6-8-3 was crossed with susceptible genotype Mingxian 169, and seedlings of parents and F1, F2, and F3 progenies were tested with prevalent Chinese P. striiformis f. sp. tritici races CYR32 and CYR33 under controlled greenhouse conditions. The genetic results indicated that two single dominant genes in H9020-1-6-8-3 confer resistance to CYR32 and CYR33, respectively. The gene for resistance to CYR33 was temporarily designated as YrH9020. Six simple-sequence repeat markers were used to map the resistance gene to the short arm of wheat chromosome 2D, using 329 F2 plants tested with CYR33 in the greenhouse. The genetic distances of the two closest flanking markers, Xgwm261 and Xgwm455, were 4.4 and 5.8 centimorgans, respectively. Disease assessments and polymorphic tests of the flanking markers among the Psathyrostachys huashanica line and wheat lines 7182, H9020-1-6-8-3, and Mingxian169 suggested that the resistance gene YrH9020 in H9020-1-6-8-3 was originally from P. huashanica. The exotic stripe rust resistance gene and linked molecular markers should be useful for pyramiding with other genes to develop wheat cultivars with high-level and durable resistance to stripe rust.

Molecular mapping of stripe rust resistance gene YrH922 in a derivative of wheat (Triticum aestivum)–Psathyrostachys huashanica

2019 ◽  
Vol 70 (11) ◽  
pp. 939
Author(s):  
Zhengwu Fang ◽  
Cai Sun ◽  
Tao Lu ◽  
Zhi Xu ◽  
Wendi Huang ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Resistance Gene ◽  
Stripe Rust ◽  
Rust Resistance ◽  
Puccinia Striiformis ◽  
Dominant Gene ◽  
Rust Resistance Gene ◽  
Stripe Rust Resistance ◽  
Psathyrostachys Huashanica ◽  
Stripe Rust Resistance Gene

Wheat stripe rust, caused by Puccinia striiformis f. sp. tritici Erikss (Pst), is one of the most damaging diseases in common wheat (Triticum aestivum L.) globally. Breeding for genetic resistance is the most effective, economical and ecologically sustainable method to control the disease. The wheat line H922-9-12, developed from a cross between Psathyrostachys huashanica Keng and T. aestivum, was highly resistant to nine Pst races in tests at the seedling stage. To characterise and map the stripe rust resistance gene(s) in H922-9-12, segregating populations were developed by crossing H922-9-12 with the susceptible cultivar Mingxian 169. When tested with Pst race CYR34, the stripe rust resistance in H922-9-12 was shown to be controlled by a single dominant gene, provisionally designated YrH922. A linkage map was constructed with five simple sequence repeat, six expressed sequence tag (EST) and two sequence-related amplified polymorphism markers. YrH922 was located on chromosome 3BL and was 2.7 and 3.4 cM proximal to EST-STS (sequence-tagged site) markers BE517923 and BE471045, respectively. The flanking marker BE517923 in marker-assisted selection for the gene can be used to improve stripe rust resistance on breeding programs.

Adult plant stripe rust resistance gene Yr71 maps close to Lr24 in chromosome 3D of common wheat

2016 ◽  
Vol 36 (7) ◽  
Author(s):  
Harbans Bariana ◽  
Kerrie Forrest ◽  
Naeela Qureshi ◽  
Hanif Miah ◽  
Matthew Hayden ◽  
...  
Keyword(s):  
Resistance Gene ◽  
Common Wheat ◽  
Stripe Rust ◽  
Rust Resistance ◽  
Rust Resistance Gene ◽  
Stripe Rust Resistance ◽  
Adult Plant ◽  
Stripe Rust Resistance Gene

Physical mapping of an adult plant stripe rust resistance gene from Triticum monococcum

2019 ◽  
Vol 29 (1) ◽  
pp. 47-55 ◽  
Author(s):  
Priti Sharma ◽  
Preeni Bawa ◽  
Bharat Yadav ◽  
Parampreet Kaur ◽  
Suruchi Jindal ◽  
...  
Keyword(s):  
Resistance Gene ◽  
Stripe Rust ◽  
Physical Mapping ◽  
Rust Resistance ◽  
Triticum Monococcum ◽  
Rust Resistance Gene ◽  
Stripe Rust Resistance ◽  
Adult Plant ◽  
Stripe Rust Resistance Gene

Molecular mapping of all stage stripe rust resistance gene YrPak in wheat landrace PI388231

Euphytica ◽  
2021 ◽  
Vol 217 (6) ◽  
Author(s):  
Maryam Tariq ◽  
Javed Iqbal Mirza ◽  
Shaukat Hussain ◽  
Naeela Qureshi ◽  
Kerrie Forrest ◽  
...  
Keyword(s):  
Resistance Gene ◽  
Stripe Rust ◽  
Rust Resistance ◽  
Molecular Mapping ◽  
Rust Resistance Gene ◽  
Stripe Rust Resistance ◽  
Wheat Landrace ◽  
Stripe Rust Resistance Gene

scholarly journals Stripe rust resistance gene Yr34 (synonym Yr48) is located within a distal translocation of Triticum monococcum chromosome 5AmL into common wheat

2021 ◽  
Author(s):  
Shisheng Chen ◽  
Joshua Hegarty ◽  
Tao Shen ◽  
Lei Hua ◽  
Hongna Li ◽  
...  
Keyword(s):  
Resistance Gene ◽  
Common Wheat ◽  
Stripe Rust ◽  
Rust Resistance ◽  
Triticum Monococcum ◽  
Rust Resistance Gene ◽  
Stripe Rust Resistance ◽  
Polyploid Wheat ◽  
Stripe Rust Resistance Gene ◽  
A Genome

AbstractKey messageThe stripe rust resistance geneYr34 was transferred to polyploid wheat chromosome 5AL from T. monococcumand has been used for over two centuries.Wheat stripe (or yellow) rust, caused by Puccinia striiformis f. sp. tritici (Pst), is currently among the most damaging fungal diseases of wheat worldwide. In this study, we report that the stripe rust resistance gene Yr34 (synonym Yr48) is located within a distal segment of the cultivated Triticum monococcum subsp. monococcum chromosome 5AmL translocated to chromosome 5AL in polyploid wheat. The diploid wheat species Triticum monococcum (genome AmAm) is closely related to T. urartu (donor of the A genome to polyploid wheat) and has good levels of resistance against the stripe rust pathogen. When present in hexaploid wheat, the T. monococcum Yr34 resistance gene confers a moderate level of resistance against virulent Pst races present in California and the virulent Chinese race CYR34. In a survey of 1,442 common wheat genotypes, we identified 5AmL translocations of fourteen different lengths in 17.5% of the accessions, with higher frequencies in Europe than in other continents. The old European wheat variety “Mediterranean” was identified as a putative source of this translocation, suggesting that Yr34 has been used for over 200 years. Finally, we designed diagnostic CAPS and sequenced-based markers that will be useful to accelerate the deployment of Yr34 in wheat breeding programs to improve resistance to this devastating pathogen.

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