scholarly journals Validating molecular markers for barley leaf rust resistance genes Rph20 and Rph24

Plant Disease ◽  
2020 ◽  
Author(s):  
P. M. Dracatos ◽  
Robert F Park ◽  
Davinder Singh
Keyword(s):  
Molecular Markers ◽  
Leaf Rust ◽  
Resistance Genes ◽  
Leaf Rust Resistance ◽  
Puccinia Hordei ◽  
Adult Plant ◽  
Barley Leaf Rust ◽  
Efficient Gene ◽  
Barley Leaf ◽  
Barley Germplasm

Improving resistance to barley leaf rust (caused by Puccinia hordei) is an important breeding objective in most barley growing regions worldwide. The development and subsequent utilization of high-throughput polymerase chain reaction (PCR) based co-dominant molecular markers remains an effective approach to select genotypes with multiple effective resistance genes, permitting efficient gene deployment and stewardship. The genes Rph20 and Rph24, which confer widely effective adult plant resistance (APR) to leaf rust, are common in European and Australian barley germplasm (often in combination), and act interactively to confer high levels of resistance. Here we report on the development and validation of co-dominant insertion-deletion (indel) based PCR markers that are highly predictive for the resistance alleles Rph20.ai and Rph24.an (both referred to as Rph20 and Rph24).

scholarly journals Validating molecular markers for barley leaf rust resistance genes Rph20 and Rph24

2020 ◽  
Author(s):  
PM Dracatos ◽  
RF Park ◽  
D Singh
Keyword(s):  
Molecular Markers ◽  
Leaf Rust ◽  
Resistance Genes ◽  
Leaf Rust Resistance ◽  
Puccinia Hordei ◽  
Adult Plant ◽  
Barley Leaf Rust ◽  
Efficient Gene ◽  
Barley Leaf ◽  
Barley Germplasm

Improving resistance to barley leaf rust (caused by Puccinia hordei) is an important breeding objective in most barley growing regions worldwide. The development and subsequent utilisation of high-throughput PCR-based co-dominant molecular markers remains an effective approach to select genotypes with multiple effective resistance genes, permitting efficient gene deployment and stewardship. The genes Rph20 and Rph24 confer widely effective adult plant resistance (APR) to leaf rust, are common in European and Australian barley germplasm (often in combination), and act interactively to confer high levels of resistance (Dracatos et al. 2015; Zeims et al. 2017; Singh et al. 2018). Here we report on the development and validation of co-dominant insertion-deletion (indel) based PCR markers that are highly predictive for the Rph20 and Rph24 resistances.

Validating molecular markers for barley leaf rust resistance genes Rph5 and Rph7

2007 ◽  
Vol 126 (5) ◽  
pp. 458-463 ◽  
Author(s):  
J. A. Mammadov ◽  
W. S. Brooks ◽  
C. A. Griffey ◽  
M. A. Saghai Maroof
Keyword(s):  
Molecular Markers ◽  
Leaf Rust ◽  
Resistance Genes ◽  
Rust Resistance ◽  
Leaf Rust Resistance ◽  
Barley Leaf Rust ◽  
Barley Leaf ◽  
Rust Resistance Genes ◽  
Leaf Rust Resistance Genes

scholarly journals First Detection of Puccinia hordei Virulence to Barley Leaf Rust Resistance Gene Rph3 and Combination with Virulence to Rph7 in North America

Plant Disease ◽  
2013 ◽  
Vol 97 (6) ◽  
pp. 838-838 ◽  
Author(s):  
M. N. Rouse ◽  
C. A. Griffey ◽  
W. S. Brooks
Keyword(s):  
North America ◽  
Leaf Rust ◽  
Rust Resistance ◽  
Leaf Rust Resistance ◽  
Puccinia Hordei ◽  
Barley Leaf Rust ◽  
Barley Leaf ◽  
Infection Types ◽  
Rust Resistance Genes ◽  
Leaf Rust Resistance Genes

Barley leaf rust, caused by Puccinia hordei Otth., has been problematic in United States barley (Hordeum vulgare L.) production in the Mid-Atlantic coast region and California. During the early 1990s, P. hordei pathotypes with virulence to resistance gene Rph7 caused average yield losses from 6 to 16% (3). ‘Doyce’ barley was released in 2003 and was described as being resistant to leaf rust (2). Initially in April 2010 and subsequently in spring 2011 and 2012, high severities and infection responses were observed on experimental plots of ‘Doyce’ in Warsaw and Blacksburg, Virginia. Three single uredinial isolates of P. hordei were derived from collections made from ‘Doyce’ barley. The isolates were characterized for virulence to barley leaf rust resistance genes by inoculating at least two replicates of a barley leaf rust differential set including 12 Rph genes (1). Previous methods used for inoculation, incubation, and pathotyping were followed (1). Infection types were scored on a 0 to 4 scale where 2 and below indicated resistance and 3 and above indicated susceptibility (4). The three isolates collected from Doyce barley displayed large pustules with infection types 3,3+ to cultivars Estate (Rph3) and Cebada Capa (Rph7). Avirulent isolates of P. hordei displayed infection types 0; to 0;1c to Estate and ;n to 0;1n to Cebada Capa (1). The data indicated that all three isolates were virulent to both barley leaf rust resistance genes Rph3 and Rph7. Though combined Rph3 and Rph7 virulence has been reported in the Mediterranean region, this is the first report of Rph3 virulence in North America. These isolates of P. hordei are virulent to important sources of resistance to barley leaf rust and threaten barley production in environments conducive for disease development in North America. References: (1) W. S. Brooks et al. Phytopathology 90:1131, 2000. (2) W. S. Brooks et al. Crop Sci. 45:792, 2005. (3) C. A. Griffey et al. Plant Dis. 78:256, 1994. (4) M. N. Levine and W. J. Cherewick. U.S. Dept. Agric. Tech. Bull. 1056, 1952.

scholarly journals Resistance against barley leaf rust (Puccinia hordei) in West-European spring barley germplasm

Agronomie ◽  
2000 ◽  
Vol 20 (7) ◽  
pp. 769-782 ◽  
Author(s):  
Rients E. Niks ◽  
Ursula Walther ◽  
Heidi Jaiser ◽  
Fernando Martinez ◽  
Diego Rubiales
Keyword(s):  
Leaf Rust ◽  
Spring Barley ◽  
Puccinia Hordei ◽  
Barley Leaf Rust ◽  
Barley Leaf ◽  
Barley Germplasm ◽  
West European

scholarly journals Genetic Analysis of Leaf Rust Resistance in Six Durum Wheat Genotypes

2014 ◽  
Vol 104 (12) ◽  
pp. 1322-1328 ◽  
Author(s):  
Alexander Loladze ◽  
Dhouha Kthiri ◽  
Curtis Pozniak ◽  
Karim Ammar
Keyword(s):  
Durum Wheat ◽  
Leaf Rust ◽  
Resistance Genes ◽  
Rust Resistance ◽  
Durable Resistance ◽  
Puccinia Triticina ◽  
Leaf Rust Resistance ◽  
Adult Plant ◽  
Seedling Resistance ◽  
Allelism Tests

Leaf rust, caused by Puccinia triticina, is one of the main fungal diseases limiting durum wheat production. This study aimed to characterize previously undescribed genes for leaf rust resistance in durum wheat. Six different resistant durum genotypes were crossed to two susceptible International Maize and Wheat Improvement Center (CIMMYT) lines and the resulting F1, F2, and F3 progenies were evaluated for leaf rust reactions in the field and under greenhouse conditions. In addition, allelism tests were conducted. The results of the study indicated that most genotypes carried single effective dominant or recessive seedling resistance genes; the only exception to this was genotype Gaza, which carried one adult plant and one seedling resistance gene. In addition, it was concluded that the resistance genes identified in the current study were neither allelic to LrCamayo or Lr61, nor were they related to Lr3 or Lr14a, the genes that already are either ineffective or are considered to be vulnerable for breeding purposes. A complicated allelic or linkage relationship between the identified genes is discussed. The results of the study will be useful for breeding for durable resistance by creating polygenic complexes.

scholarly journals Mapping genes for resistance to Puccinia hordei in barley

2003 ◽  
Vol 54 (12) ◽  
pp. 1323 ◽  
Author(s):  
R. F. Park ◽  
D. Poulsen ◽  
A. R. Barr ◽  
M. Cakir ◽  
D. B. Moody ◽  
...  
Keyword(s):  
Resistance Genes ◽  
Doubled Haploid ◽  
Recombinant Inbred ◽  
Growth Stage ◽  
Population Based ◽  
Puccinia Hordei ◽  
Barley Leaf Rust ◽  
Recombinant Inbred Population ◽  
Rust Pathogen ◽  
Barley Leaf

Six doubled haploid barley populations (Alexis × Sloop, Chebec × Harrington, Arapiles × Franklin, Patty × Tallon, Tallon × Kaputar, and Sloop × Halcyon) and a recombinant inbred population (WI2875-1 × Alexis) were assessed for response to selected pathotypes of the barley leaf rust pathogen, Puccinia hordei, at the seedling growth stage. Resistance genes were postulated for the parents of each population based on their reaction to selected pathotypes. In most cases, the resistance genes postulated in the cultivars were validated by QTL mapping analyses of the progeny populations. The resistance genes detected and mapped were Rph2, Rph3, Rph4, Rph12, and Rph19. The chromosomal locations of these 5 genes were consistent with previous reports, with Rph2 mapping near to the centromere on the short arm of chromosome 5H, Rph4 mapping to chromosome 1H, Rph12 mapping to the long arm of chromosome 5H, and Rph3 and Rph19 mapping ~30 cM apart on the long arm of chromosome 7H.

Molecular markers for four leaf rust resistance genes introgressed into wheat from wild relatives

Genome ◽  
1995 ◽  
Vol 38 (1) ◽  
pp. 75-83 ◽  
Author(s):  
Enrique Autrique ◽  
Steven D. Tanksley ◽  
Mark E. Sorrells ◽  
Ravi P. Singh
Keyword(s):  
Molecular Markers ◽  
Leaf Rust ◽  
Resistance Genes ◽  
Leaf Rust Resistance ◽  
Distal Portion ◽  
Rflp Markers ◽  
Aegilops Umbellulata ◽  
Breeding Populations ◽  
Aneuploid Analysis ◽  
Homoeologous Chromosomes

Near-isolines carrying four different genes for resistance to leaf rust were used to find linked molecular markers for these genes. Clones used to detect polymorphism were selected on the basis of the reported chromosomal location of the resistance genes. Both Lophopyron-derived resistance genes, Lr19 and Lr24, cosegregated with eight molecular markers assigned to chromosomes 7DL and 3DL, respectively. One clone cosegregated with Lr9 and two closely linked RFLP markers were found for Lr32, mapping at 3.3 ± 2.6 and 6.9 ± 3.6 cM from the resistance gene. The Lophopyron-chromatin segment in isolines carrying chromosomes 7E (Lr19) and 3E (Lr24) replaced a large portion of chromosome 7D and the distal portion of chromosome 3D, respectively. Clones assigned to these chromosomes on the basis of aneuploid analysis hybridized to 7E and 3E segments, thus confirming cytological results that these introgressed segments represent homoeologous chromosomes. The linked RFLP markers could be used to identify the resistance genes and generate new combinations in breeding populations, especially in the absence of disease in the environment or when virulence is lacking.Key words: leaf rust, RFLP, Lophopyron, gene tagging, wheat, Aegilops umbellulata, Triticum tauschii.

Transfer to hexaploid wheat of linked genes for adult-plant leaf rust and seedling stem rust resistance from an amphiploid of Aegilops speltoides × Triticum monococcum

Genome ◽  
1990 ◽  
Vol 33 (4) ◽  
pp. 530-537 ◽  
Author(s):  
E. R. Kerber ◽  
P. L. Dyck
Keyword(s):  
Leaf Rust ◽  
Resistance Genes ◽  
Stem Rust ◽  
Rust Resistance ◽  
Dominant Gene ◽  
Leaf Rust Resistance ◽  
Stem Rust Resistance ◽  
Adult Plant ◽  
Aegilops Speltoides ◽  
Plant Leaf

A partially dominant gene for adult-plant leaf rust resistance together with a linked, partially dominant gene for stem rust resistance were transferred to the hexaploid wheat cultivar 'Marquis' from an amphiploid of Aegilops speltoides × Triticum monococcum by direct crossing and backcrossing. Pathological evidence indicated that the alien resistance genes were derived from Ae. speltoides. Differential transmission of the resistance genes through the male gametes occurred in hexaploid hybrids involving the resistant 'Marquis' stock and resulted in distorted segregation ratios. In heterozygotes, pairing between the chromosome arm with the alien segment and the corresponding arm of the normal wheat chromosome was greatly reduced. The apparent close linkage between the two resistance genes, 3 ± 1.07 crossover units, was misleading because of this decrease in pairing in the presence of the 5B diploidizing mechanism. The newly identified gene for adult-plant leaf rust resistance, located on chromosome 2B, is different from adult-plant resistance genes Lr12, Lr13, and Lr22 and from that in the hexaploid accession PI250413; it has been designated Lr35. It is not known whether the newly transferred gene for stem rust resistance differs from Sr32, also derived from Ae. speltoides and located on chromosomes 2B.Key words: hexaploid, Triticum, Aegilops, aneuploid, Puccinia graminis, Puccinia recondita.

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