Dianthus chinensis is a popular ornamental plant that is widely cultivated in China. From May 2020 to 2021, root rot and foliage blight were observed on approximately 50% groundcover plants at several landscape sites of Xuanwuhu Park and Nanjing Railway Station, China. Symptoms of wilting and chlorosis appeared in the initial stage, and severe infection caused the whole plant to die . To recover the causal pathogen, infected root and leaf samples were cut into 5×5 mm2 squares, surface-disinfected in 70% ethanol for 30 sec, placed onto 10% clarified V8 PARP agar at 25°C . After three days, Phytophthora-like hyphae were visibly emerged from both root and leaf tissues and growing into cV8A. Individual hyphal tips were transferred to new cV8A plates to obtain a total of 10 pure isolates. Colony morphology of all isolates on cV8A had slightly radiate to stellate patterns with cottony aerial mycelia. After four or five days all isolates had identical morphological traits including papillate and noncaducous sporangia on cV8, hyphal swellings, and intercalary and terminal chlamydospores. A representative isolate Pni-dc7 was examined for morphological measurements. Sporangia were mostly ovoid and sometimes obpyriform, averaging 28.9±5.6 µm in length and 24.9±5.8 µm in width (n=30). Chlamydospores were abundant and spherical with an average diameter at 29.2 ± 0.3 µm (n=30). Oogonia were not observed. For sequence analysis, the internal transcribed spacer (ITS) regions and large subunit (LSU) of the nuclear ribosomal RNA gene complex were amplified using the primer pairs ITS1/ITS4 and NL1/NL4 , respectively, while the mitochondrial cytochrome c oxidase subunit II (coxII) gene was amplified using FM58/FM66 (Martin et al. 2003). The ITS sequence of isolate Pni-dc7 (GenBank Acc. No. MZ519893) had a 100% identity to those of P. nicotianae (MH219914, KU172524, MT065839). The LSU sequence (MZ573547) had a 100% identity to those of P. nicotianae (KX250514, MZ348950, HQ665198).The cox2 sequence (MZ519893) had a 100% identity to those of P. nicotianae (MH221078, KJ506439, JF707072). Based on morphological and molecular evidence, Pni-dc7 was identified as P. nicotianae. Pathogenicity tests were conducted using both detached leaves and whole plants. Asymptomatic leaves were collected from healthy plants.A 5×5 mm2 Pni-dc7-colonized cV8A plug was placed on each wound of five leaves. Sterile agar plugs were used for a non-inoculated control leaf. All six leaves were placed on a wet filter paper in a closed container at 25°C. All inoculated leaves had necrotic tissues around the wounds, the symptoms progressed from spots to the entire leaves after two days . The control leaves remained asymptomatic. In the whole-plant assay, a D. chinensis plant (approx. 0.3 m in height) was inoculated with 5 mL of zoospore suspension that was mixed into the potting soil(500g). Three plants were inoculated and control plants were treated with sterile distilled water. After two weeks all three inoculated plants in three repeats of the assay had root and crown rot and foliage blight, whereas all control plants remained asymptomatic. P. nicotianae was reisolated from all inoculated plants. This is the first report of P. nicotianae causing root rot and foliage blight on D. chinensis in China. Considering the importance of D. chinensis to both ornamental nursery and landscaping industries in China, diseased plants at the landscape sites were removed to prevent the spread of P. nicotianae to production sites and other landscape locations.
First Report of Phytopythium sp. Causing Storage Root Rot and Foliage Blight of Cassava in Brazil