scholarly journals Xiphinema rivesi from Chile Transmits Tomato ringspot virus to Cucumber

Plant Disease ◽  
2009 ◽  
Vol 93 (9) ◽  
pp. 971-971 ◽  
Author(s):  
J. Auger ◽  
G. Leal ◽  
J. C. Magunacelaya ◽  
M. Esterio
Keyword(s):  
Ringspot Virus ◽  
Feeding Period ◽  
Tomato Ringspot Virus ◽  
Virus Acquisition ◽  
Bait Plants ◽  
Systemic Symptoms ◽  
High Virus ◽  
Leaf Virus ◽  
Virus Isolates ◽  
Das Elisa

The dagger nematode, (Xiphinema rivesi Dalmasso), a member of the X. americanum group, was first reported in 2002 in Chile (3). X. rivesi is a vector of at least four North American nepoviruses including Cherry rasp leaf virus (CRLV), Tobacco ringspot virus (TobRSV), Tomato ringspot virus (TomRSV), and Peach rosette mosaic virus (PRMV) (2). TomRSV, first reported in Chile in 1984, was associated with raspberry decline and lately with brownline disease in D'Agen prune trees (1), however none of the Xiphinema spp. found in Chile have been reported to transmit this nepovirus. Two virus isolates, TomRSV (prune brownline isolate PBL-08) and Grapevine fanleaf virus (GFLV) (Yellow mosaic isolate GFLV-012), from the virus collection of the Departamento de Sanidad Vegetal, Universidad de Chile were used in transmission tests with a population of X. rivesi found in Chile. X. rivesi is not known to transmit GFLV and this virus was included as a check. The nematodes were extracted from soil from a D'Agen prune orchard, and transmission tests were done in compliance with the criteria proposed by Trudgill et al. (4). Cucumis sativus cv. National Pickling were grown in a growth chamber at 25°C and used as acquisition hosts and transmission bait plants. Acquisition hosts were mechanically inoculated with GFLV or TomRSV, displaying systemic symptoms in 15 to 20 days. Noninoculated cucumber plants were included as controls. Virus infection was confirmed by double-antibody sandwich (DAS)-ELISA before the introduction of nematodes to the soil. After a 20-day acquisition feeding period, the nematodes were wet screened from the soil and added to the healthy bait plants and allowed a 20-day inoculation feeding period. X. rivesi transmitted TomRSV but not GFLV. TomRSV bait plants developed systemic symptoms 5 weeks after the nematodes were transferred. Transmission of TomRSV was confirmed by testing leaf and root sap of bait plants in a DAS-ELISA. High virus concentrations were detected in the roots and leaves of TomRSV symptomatic plants. Bait plants on which nematodes had been allowed to feed following virus acquisition from GFLV-infected or from virus-free control plants tested negative by ELISA. No symptoms appeared on bait plants used for GFLV transmission or the control bait plants. To our knowledge, this is the first report of transmission of TomRSV with a Xiphinema population from Chile and South America. References: (1) J. Auger. Acta Hortic. 235:197, 1988. (2) D. J. F. Brown et al. Phytopathology 84:646, 1994. (3) G. Leal et al. Fitopatología 37:75, 2002. (4) D. L. Trudgill et al. Rev. Nematol. 6:133, 1983.

scholarly journals Xiphinema rivesi from Slovania Transmit Tobacco ringspot virus and Tomato ringspot virus to Cucumber Bait Plants

Plant Disease ◽  
2007 ◽  
Vol 91 (6) ◽  
pp. 770-770 ◽  
Author(s):  
S. Širca ◽  
B. Geric Stare ◽  
I. Mavrič Pleško ◽  
M. Viršček Marn ◽  
G. Urek ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Plant Protection ◽  
Ringspot Virus ◽  
Feeding Period ◽  
Arabis Mosaic Virus ◽  
Tobacco Ringspot Virus ◽  
Tomato Ringspot Virus ◽  
Bait Plants ◽  
Systemic Symptoms ◽  
Das Elisa

The dagger nematode, Xiphinema rivesi Dalmasso, a member of the X. americanum group, was detected in 2002 for the first time in Slovenia and for the fourth time in Europe (4). X. rivesi is a vector of at least four North American nepoviruses including Cherry rasp leaf virus (CRLV), Tobacco ringspot virus (TRSV), Tomato ringspot virus (ToRSV), and Peach rosette mosaic virus (PRMV) (1,2). All of these viruses are included on the EPPO and EU lists of quarantine organisms, but none of the Xiphinema species found in Europe have been reported to transmit these nepoviruses. Three virus isolates, including TRSV (from Lobelia spp.; virus collection of the Plant Protection Service, Wageningen, The Netherlands), ToRSV (grapevine isolate PV-0381; DSMZ, Braunschweig, Germany), and Arabis mosaic virus (ArMV) (from Vinca spp.; virus collection of the Plant Protection Service), were used in transmission tests with a population of X. rivesi found in Slovenia. X. rivesi is not known to transmit ArMV and this virus was included as a check. The nematodes were extracted from peach orchard soil collected near the village of Dornberk, and transmission tests fulfilled the set of criteria proposed by Trudgill et al. (3). Cucumis sativus cv. Eva, grown in a growth chamber at 25°C, was used as acquisition hosts and transmission bait plants. The acquisition hosts were mechanically inoculated and showing systemic symptoms before the introduction of nematodes. Noninoculated acquisition plants were included as controls. After a 10-day acquisition feeding period, the nematodes were transferred to healthy bait plants and allowed a 14-day inoculation feeding period. X. rivesi transmitted TRSV and ToRSV but not ArMV. TRSV and ToRSV bait plants developed systemic symptoms 4 to 6 weeks after the nematodes were transferred. Transmission of TRSV and ToRSV was confirmed by testing leaf and root sap of bait plants in a double antibody sandwich (DAS)-ELISA. High virus concentrations were detected in the roots and leaves of TRSV and ToRSV symptomatic plants. DAS-ELISA on bait plants from nematodes that had been allowed to feed on ArMV-infected or the virus-free control acquisition plants gave negative results. No symptoms appeared on bait plants used for ArMV transmission or the control bait plants. To our knowledge, this is the first report of transmission of TRSV and ToRSV with a Xiphinema population from Europe. References: (1) D. J. F. Brown et al. Phytopathology 84:646, 1994. (2) L.W. Stobbs et al. Plant Dis. 80:105, 1996. (3) D. L. Trudgill et al. Rev. Nematol. 6:133, 1983. (4) G. Urek et al. Plant Dis. 87:100, 2002.

A Survey for Nine Major Viruses of Grapevines in Tennessee Vineyards

2020 ◽  
Vol 21 (3) ◽  
pp. 157-161
Author(s):  
Nourolah Soltani ◽  
Rongbin Hu ◽  
Darrell D. Hensley ◽  
David L. Lockwood ◽  
Keith. L. Perry ◽  
...  
Keyword(s):  
First Record ◽  
Ringspot Virus ◽  
Rt Pcr ◽  
Grapevine Fanleaf Virus ◽  
Arabis Mosaic Virus ◽  
Growing Seasons ◽  
Tomato Ringspot Virus ◽  
The Status ◽  
Diagnostic Approaches ◽  
Das Elisa

Despite the significance of grape production to the fruit industry in Tennessee (TN), no published information has been available on viruses affecting grapevines in the state. Hence, a survey was conducted during the 2016 and 2017 growing seasons to determine the status of nine major viruses of grapevines in TN vineyards by taking advantage of classical serological assays and confirmatory nucleic acid-based diagnostic approaches. A total of 349 samples from 23 grapevine cultivars mostly displaying viral-like symptoms were collected from 23 commercial vineyards. All samples were assayed by DAS-ELISA for arabis mosaic virus (ArMV), grapevine leafroll-associated virus (GLRaV)-1, GLRaV-2, GLRaV-3, GLRaV-4, grapevine fanleaf virus (GFLV), tobacco ringspot virus (TRSV), and tomato ringspot virus (ToRSV). Selected serologically positive samples were also tested by RT-PCR, followed by Sanger sequencing of the generated amplicons. Additionally, 19 grapevines displaying symptoms characteristic of grapevine red blotch virus (GRBV) were also assayed by PCR followed by confirmatory sequencing-based methods. Collectively, these assays verifiably detected GLRaV-1, GLRaV-2, GLRaV-3, ToRSV, and GRBV in TN vineyards. This is the first record of the presence of these viruses in TN vineyards. ArMV, GLRaV-4, GFLV, and TRSV were not detected. The majority of samples tested positive for a single virus, whereas mixed infections with more than one virus were detected in 37% of samples.

Characterization of the partial RNA1 and RNA2 3′ untranslated region of Tomato ringspot virus isolates from North America

2011 ◽  
Vol 33 (1) ◽  
pp. 94-99 ◽  
Author(s):  
Ruhui Li ◽  
Ray Mock ◽  
Marc Fuchs ◽  
John Halbrendt ◽  
Bill Howell ◽  
...  
Keyword(s):  
North America ◽  
Untranslated Region ◽  
Ringspot Virus ◽  
Tomato Ringspot Virus ◽  
Virus Isolates

scholarly journals First Report of the Dagger Nematode Xiphinema rivesi, a Member of the X. americanum Group, from Slovenia

Plant Disease ◽  
2003 ◽  
Vol 87 (1) ◽  
pp. 100-100 ◽  
Author(s):  
G. Urek ◽  
S. Širca ◽  
L. Kox ◽  
G. Karssen
Keyword(s):  
Plant Protection ◽  
Fruit Production ◽  
Early Spring ◽  
Ringspot Virus ◽  
Polymorphism Analysis ◽  
Pome Fruit ◽  
First Report ◽  
Tomato Ringspot Virus ◽  
Fourth Report ◽  
Leaf Virus

The dagger nematode, Xiphinema rivesi Dalmasso, is a virus-transmitting plant-parasitic nematode described from France (2) and also reported from Germany, Portugal, and Spain. While this species is only known from a few local sites within Europe, it is widespread in North America and also present in Peru and Pakistan (3). X. rivesi is able to transmit at least four North American nepoviruses, including Cherry rasp leaf virus, Tobacco ringspot virus, Tomato ringspot virus, and Peach rosette mosaic virus (1,4). In early spring 2002, nematodes of the X. americanum group were detected in soil samples taken near the roots of peach plants from an orchard in Bilje near Nova Gorica, Slovenia. Resampling in June and September at the same site and at an adjacent, approximately 30-year-old peach orchard confirmed the presence of the nematode in relatively high numbers (5 per 100 ml of soil). Adult females were isolated and sent for further identification to the Plant Protection Service in Wageningen, the Netherlands. The nematode was morphologically identified as X. rivesi and confirmed at the rDNA-level with internal transcribed spacer restriction fragment length polymorphism analysis. Because both sampling sites where the nematode was found are close to the Vipava River in an area of extensive stone and pome fruit production, it is likely that the nematode is also present in other orchards in this region. The origin of this quarantine nematode is unknown as there is no direct link with Slovenian orchards and import of plant material from abroad in the last decades. To our knowledge, this is the first report of X. rivesi in Slovenia and the fourth report of this species in Europe. References: (1) D. J. F. Brown et al. Phytopathology 84:646, 1994. (2) A. Dalmasso. Mem. Mus. Hist. Nat. Nouv. Série A., Zool. 61:33, 1969. (3) Data Sheets on Quarantine Pests: Xiphinema americanum sensu lato. Page 629 in: Quarantine Pest for Europe, CABI, Wallingford, UK, 1997. (4) L. W. Stobbs et al. Plant Dis. 80:105, 1996.

scholarly journals First Report of Tomato ringspot virus Infecting Pepper in Iran

Plant Disease ◽  
2012 ◽  
Vol 96 (12) ◽  
pp. 1828-1828 ◽  
Author(s):  
Y. Sokhansanj ◽  
F. Rakhshandehroo ◽  
R. Pourrahim
Keyword(s):  
Mosaic Virus ◽  
Ringspot Virus ◽  
Local Lesion ◽  
Capsicum Frutescens ◽  
Polymerase Gene ◽  
Rt Pcr ◽  
Viral Polymerase ◽  
First Report ◽  
Tomato Ringspot Virus ◽  
Das Elisa

Chili pepper (Capsicum frutescens) represents an important crop in Iran and is under cultivation in different regions in Northern Iran. In spring 2012, commercially grown tabasco (Capsicum frutescens) peppers in Varamin, Shahriar, and Karaj districts of Tehran province developed an undescribed disease. Symptoms observed were mosaic, leaf malformations, and stunting. Fruit symptoms included chlorosis and distortion. To verify the identity of the disease, six fields were surveyed and 72 symptomatic leaves were collected and screened by double antibody sandwich (DAS)-ELISA using specific antibodies to Tobacco ringspot virus (TRSV), Tomato ringspot virus (ToRSV), Pepper mild mottle virus (PMMV), Tomato mosaic virus (ToMV), Tobacco mosaic virus (TMV), and Arabis mosaic virus (ArMV). ToRSV was found in 23% of the samples collected. None of the samples had a positive reaction to other tested viruses. The ToRSV-positive peppers were used for mechanical transmission to Chenopodium quinoa, local lesion host, and after two cycles of single local lesion isolation, they were transferred to Cucumis sativus, Solanum esculentum, and Capsicum fructescens. Inoculations resulted in systemic mosaic and chlorotic local lesion on C. sativus; leaf distortion and mosaic on S. esculentum; and mosaic, mottle, and stunting on C. fructescens. All inoculated plants were positive for ToRSV with DAS-ELISA. To further verify ToRSV infection, reverse transcription (RT)-PCR was conducted. Two primers were designed on the basis of the highly conserved sequences of the putative viral polymerase gene available in the GenBank. RT-PCR of total RNA extract from infected peppers and inoculated plants with the designed primers RdR-R (5′-CGCCTGGTAATTGAGTAGCCC-3′) and RdR-F (5′-GAAGAGCTAGAGCCTCAACCAGG-3′), consistently amplified the 411-bp product, while no amplification products were obtained from noninfected control (healthy plants). The fragment from tabasco pepper was cloned into pTZ57R/T (Ins T/A clone PCR Cloning kit, Fermentas, St. Leon-Rot, Germany) and sequenced in both directions of three clones. The resulting nucleotide sequence (GenBank Accession No. JQ972695) had the highest identity (94%) with the polymerase gene of a ToRSV isolate from blueberry cv. Patriot (Accession No. GQ141528) and had lower identity (91%) with that of a ToRSV isolate from blueberry cv. Bluecrop (Accession No. GQ141525). Tomato ringspot virus (ToRSV) is reported to infect Capsicum spp. in the United States (1,2). Our results confirm the natural infection of pepper plants in Tehran by ToRSV. To our knowledge, this is the first report of ToRSV infection of pepper in Iran. The finding of this disease in Tehran confirms further spread of the virus within northern regions of Iran and prompts the need for research to develop more effective management options to reduce the impact of ToRSV on pepper crops. Beside, primers designed on the basis of putative viral polymerase gene sequences may improve the detection of ToRSV isolates by RT-PCR in Iran. References: (1) S. K. Green and J. S. Kim. Technical Bulletin. No.18, 1991. (2) G. P. Martelli and A. Quacquarelli. Acta Hortic. 127:39, 1983.

Complete genome sequence of three tomato ringspot virus isolates: evidence for reassortment and recombination

2014 ◽  
Vol 160 (2) ◽  
pp. 543-547 ◽  
Author(s):  
Melanie Walker ◽  
Joan Chisholm ◽  
Ting Wei ◽  
Basudev Ghoshal ◽  
Hanna Saeed ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Ringspot Virus ◽  
Tomato Ringspot Virus ◽  
Virus Isolates

scholarly journals Caracterização do Tomato chlorotic spot virus isolado de jiló no Vale do Paraíba, Estado de São Paulo

2002 ◽  
Vol 27 (3) ◽  
pp. 285-291 ◽  
Author(s):  
MARCELO EIRAS ◽  
ALEXANDRE L. R. CHAVES ◽  
ADDOLORATA COLARICCIO ◽  
RICARDO HARAKAVA ◽  
JANSEN DE ARAUJO ◽  
...  
Keyword(s):  
Tomato Spotted Wilt Virus ◽  
Sao Paulo ◽  
Ringspot Virus ◽  
São Paulo ◽  
Rt Pcr ◽  
Spot Virus ◽  
Chlorotic Spot ◽  
Tomato Spotted Wilt ◽  
Wilt Virus ◽  
Das Elisa

Os tospovírus são responsáveis por perdas significativas em diversas culturas, principalmente solanáceas. No município de São José dos Campos (SP), plantas de jiló (Solanum gilo) apresentando sintomas de mosaico, bolhosidades, nanismo e queda acentuada da produção foram coletadas para análise. Visando a caracterização do agente causador dos sintomas, testes biológicos, elétrono microscópicos, sorológicos e moleculares foram realizados. Através de inoculação mecânica em plantas indicadoras das famílias Amaranthaceae, Chenopodiaceae e Solanaceae obtiveram-se resultados típicos aos esperados para tospovírus. Ao microscópio eletrônico de transmissão, observaram-se, em contrastação negativa, partículas pleomórficas com diâmetro entre 80 e 110 nm e em cortes ultra-finos partículas presentes em vesículas do retículo endoplasmático. Através de DAS-ELISA, identificou-se o Tomato chlorotic spot virus (TCSV). A partir de RNA total extraído de folhas infetadas, amplificaram-se, via RT-PCR, fragmentos correspondentes ao gene da proteína do capsídeo (cp) os quais foram seqüenciados e comparados com outros depositados no "GenBank". A homologia de nucleotídeos e aminoácidos deduzidos foi respectivamente de 99 e 95% quando comparada com seqüências de isolados de TCSV. A comparação com as outras espécies do gênero Tospovirus apresentou valores de homologia entre 72 e 84%. Estes resultados confirmam a identidade deste vírus como pertencente à espécie TCSV, que é predominante no Estado de São Paulo e importante patógeno de outras plantas cultivadas. Além disso, variedades de jiló quando inoculadas foram susceptíveis tanto ao TCSV como às espécies Tomato spotted wilt virus (TSWV) e Groundnut ringspot virus (GRSV).

scholarly journals Complete Genome Sequences of Two Hydrangea Ringspot Virus Isolates from Japan

2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Akira Yusa ◽  
Nozomu Iwabuchi ◽  
Hiroaki Koinuma ◽  
Takuya Keima ◽  
Yutaro Neriya ◽  
...  
Keyword(s):  
Complete Genome ◽  
Rna Virus ◽  
Nucleotide Sequences ◽  
Ringspot Virus ◽  
Genomic Sequences ◽  
Genome Sequences ◽  
Hydrangea Macrophylla ◽  
Hydrangea Ringspot Virus ◽  
Virus Isolates

Hydrangea ringspot virus (HdRSV) is a plant RNA virus, naturally infecting Hydrangea macrophylla . Here, we report the first genomic sequences of two HdRSV isolates from hydrangea plants in Japan. The overall nucleotide sequences of these Japanese isolates were 96.0 to 96.3% identical to those of known European isolates.

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