First Report of Columbia Root-Knot Nematode (Meloidogyne chitwoodi) in Potato in New Mexico

Following a report of Columbia root-knot nematode in potatoes (Solanum tuberosum L.) imported by Mexico from the United States in spring 2000, six fields in San Juan County, NM, were surveyed in August 2000. Soil samples from two fields in which the exported potatoes had been produced contained second-stage juveniles that were tentatively identified as Columbia root-knot nematode. During the 2000 potato harvest, state inspectors detected tubers from four additional fields that exhibited symptoms of Columbia root-knot nematode, including warty exteriors and discrete small brown lesions that were apparent to a depth of 1 cm below the tuber surface. Meloidogyne chitwoodi Golden et al. (1) was confirmed from a subsample of tubers sent to the USDA Nematology Laboratory in Beltsville, MD, in October 2000. Identification was based on morphological examination of the nematodes recovered from tubers. To our knowledge, this is the first report of M. chitwoodi from New Mexico. No Columbia root-knot nematodes were recovered from soil samples collected at 26 locations throughout San Juan County in 1988 and 1989, nor had symptomatic tubers or plants been observed in this area previously. Columbia root-knot nematode most likely represents a recent introduction into northwestern New Mexico. Additional information regarding distribution of this nematode within the region is needed. References: (1) A. M. Golden et al. J. Nematol. 12:319–327, 1980.

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First Report of Meloidogyne partityla on Pecan in New Mexico

Plant Disease ◽

10.1094/pdis.2001.85.9.1030b ◽

2001 ◽

Vol 85 (9) ◽

pp. 1030-1030 ◽

Cited By ~ 6

Author(s):

S. H. Thomas ◽

J. M. Fuchs ◽

Z. A. Handoo

Keyword(s):

New Mexico ◽

The United States ◽

Root Knot Nematode ◽

Carya Illinoensis ◽

Morphological Examination ◽

Egg Masses ◽

First Report ◽

Additional Information ◽

Dona Ana County ◽

Normal Fertilization

For several years, decline was observed in mature pecan (Carya illinoensis (F.A. Wangenheim) K. Koch) trees in an orchard in Dona Ana County, New Mexico despite normal fertilization and irrigation practices. Affected trees were growing in sandy soil in two widely separated irrigation terraces and exhibited chlorosis of foliage and substantial die-back of branches in the upper canopy. Examination of feeder roots revealed the presence of numerous small galls and egg masses, with root-knot nematode females often visibly protruding from root tissue. Attempts to culture the nematode on tomato (Lycopersicon esculentum Mill. ‘Rutgers’) were unsuccessful. Females and egg masses were collected from fresh pecan roots and sent to the USDA Nematology Laboratory in Beltsville, MD, in October 2000, where specimens were identified as Meloidogyne partityla Kleynhans (1) based on morphological examination. This is the first report of M. partityla from New Mexico, and the second report of this nematode outside South Africa. Starr et al. (2) first reported M. partityla from pecan in the United States in 1996, after recovering the nematode from five orchards in Texas. In their study, the host range of M. partityla was limited to members of the Juglandaceae, which may explain the inability of the New Mexico population to reproduce on tomato. Additional information is needed regarding distribution of this nematode within pecan-growing regions throughout North America. References: (1) K. P. N. Kleynhans. Phytophylactica 18:103, 1986. (2) J. L. Starr et al. J. Nematol. 28:565, 1996.

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First Report of Columbia Root Knot Nematode (Meloidogyne chitwoodi) in Potato in Texas

Plant Disease ◽

10.1094/pdis.2001.85.4.442d ◽

2001 ◽

Vol 85 (4) ◽

pp. 442-442 ◽

Cited By ~ 1

Author(s):

A. L. Szalanski ◽

P. G. Mullin ◽

T. S. Harris ◽

T. O. Powers

Keyword(s):

New Mexico ◽

Solanum Tuberosum L ◽

Root Knot Nematode ◽

Chain Reaction ◽

Meloidogyne Chitwoodi ◽

Its1 Region ◽

First Report ◽

Tail Shape ◽

Central United States ◽

Polymerase Chain

Columbia root-knot nematode, Meloidogyne chitwoodi Golden et al. (1) was identified from potatoes, Solanum tuberosum L., collected from Dallam County, Texas in October 2000. Seed potatoes are the most likely source for this introduction. This nematode is currently found infecting potatoes grown in California, Colorado, Idaho, New Mexico, Nevada, Oregon, Utah, and Washington. Some countries prohibit import of both seed and table stock potatoes originating in states known to harbor M. chitwoodi. Lesions on the potatoes had discrete brown coloration with white central spots in the outer 1 cm of the tuber flesh. Female nematode densities averaged 3 per square centimeter of a potato section beneath the lesions. Nematodes were morphologically identified as M. chitwoodi based on the perineal pattern of mature females and the tail shape of juveniles per Golden et al. (1). Using polymerase chain reaction-RFLP of the rDNA ITS1 region and the mtDNA COII-16S rRNA region (2), individual juveniles were identified as M. chitwoodi based on their restriction fragment patterns. This is the first report of Columbia root-knot nematode infecting potatoes in Texas. The distribution of this nematode in potato fields throughout central United States should be determined. References: (1) A. N. Golden et al. J. Nematol. 12:319, 1980. (2) T. O. Powers and T. S. Harris. J. Nematol. 25:1, 1993.

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First Report of Columbia Root-Knot Nematode (Meloidogyne chitwoodi) in Potato in Turkey

Plant Disease ◽

10.1094/pdis-93-3-0316c ◽

2009 ◽

Vol 93 (3) ◽

pp. 316-316 ◽

Cited By ~ 8

Author(s):

A. Ozarslandan ◽

Z. Devran ◽

N. Mutlu ◽

I. H. Elekcioglu

Keyword(s):

Restriction Fragment ◽

Solanum Tuberosum L ◽

Intergenic Spacer ◽

The United States ◽

Root Knot Nematode ◽

Meloidogyne Chitwoodi ◽

First Report ◽

Intergenic Spacer Region ◽

Damage Probability ◽

United States Mexico

Columbia root-knot nematode, Meloidogyne chitwoodi Golden et al., was identified from potatoes, Solanum tuberosum L., collected from Nigde Province, Turkey in September 2006. Seed potatoes are the most likely source for this introduction. The nematode is currently found to be infecting potatoes grown in the Netherlands, Portugal, Belgium, Germany, the United States, Mexico, South Africa, and Argentina. M. chitwoodi acquired a quarantine status in Europe (1) because of its potential to become established worldwide and its high damage probability. Some countries prohibit import of both seed and table stock potatoes originating in states known to harbor M. chitwoodi. Lesions on the potatoes had discrete brown coloration with white central spots in the outer 1 cm of the tuber flesh. Female nematode densities averaged 3 to 5 per cm2 of a potato section beneath the lesions. Nematodes were morphologically identified as M. chitwoodi based on the perineal pattern of mature females and the tail shape of juveniles. Using PCR-restriction fragment length polymorphism of the 18S region (3) and the mtDNA COII-16S rRNA region (2) and intergenic spacer region between the 5S and 18S genes (4), individual juveniles were identified as M. chitwoodi based on their restriction fragment patterns. To our knowledge, this is the first report of Columbia root-knot nematode infecting potatoes in Turkey. The distribution of this nematode in potato fields throughout Turkey should be determined. References: (1) L. J. M. F. Den Nijs et al. Nematology 6:303, 2004. (2) T. O. Powers and T. S. Harris. J. Nematol. 25:1, 1993. (3) T. O. Powers et al. J. Nematol. 37:226, 2005. (4) J. Wishart et al. Phytopathology 92:884, 2002.

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