scholarly journals First Report of Raspberry bushy dwarf virus in Rubus multibracteatus from China

Plant Disease ◽  
2003 ◽  
Vol 87 (5) ◽  
pp. 603-603 ◽  
Author(s):  
C. J. Chamberlain ◽  
J. Kraus ◽  
P. D. Kohnen ◽  
C. E. Finn ◽  
R. R. Martin
Keyword(s):  
Amino Acid ◽  
Coat Protein ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Dwarf Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Black Raspberry ◽  
First Report ◽  
Raspberry Bushy Dwarf Virus ◽  
Plant Pathol

Raspberry bushy dwarf virus (RBDV), genus Idaeovirus, has been reported in commercial Rubus spp. from North and South America, Europe, Australia, New Zealand, and South Africa. Infection can cause reduced vigor and drupelet abortion leading to crumbly fruit and reduced yields (3,4). In recent years, Rubus germplasm in the form of seed, was obtained on several collection trips to The People's Republic of China to increase the diversity of Rubus spp. in the USDA-ARS National Clonal Germplasm Repository, (Corvallis, OR). Before planting in the field, seedlings were tested for the presence of RBDV, Tomato ringspot virus, and Tobacco streak virus using triple-antibody sandwich enzyme-linked immunosorbent assay (TAS-ELISA) (antiserum produced by R. R. Martin). One symptomless plant of R. multibracteatus H. Lev. & Vaniot (PI 618457 in USDA-ARS GRIN database), from Guizhou province in China, tested positive for RBDV (RBDV-China). After mechanical transmission on Chenopodium quinoa Willd., this isolate produced typical symptoms of RBDV (3). To determine if RBDV-China was a contaminant during the handling of the plants, or if the source was a seedborne virus, the coat protein gene was sequenced and compared to published sequences of RBDV. RNA was extracted from leaves of R. multibracteatus and subjected to reverse transcription-polymerase chain reaction (RT-PCR) using primers that flank the coat protein gene. Products from four separate PCR reactions were sequenced directly or were cloned into the plasmid vector pCR 2.1 (Invitrogen, Carlsbad, CA) and then sequenced. The coding sequence of the coat protein gene of RBDV-China was 87.5% (722/825) identical to that isolated from black raspberry (Genbank Accession No. s55890). The predicted amino acid sequences were 91.6% (251/274) identical. Previously, a maximum of five amino acid differences had been observed in the coat proteins of different RBDV strains (1). The 23 differences observed between RBDV-China and the isolate from black raspberry (s55890) confirm that the RBDV in R. multibracteatus is not a greenhouse contaminant but is indeed a unique strain of RBDV. In addition, monoclonal antibodies (MAbs) to RBDV (2) were tested against RBDV-China. In these tests, MAb D1 did not detect RBDV-China, whereas MAb R2 and R5 were able to detect the strain. This is the first strain of RBDV that has been clearly differentiated by MAbs using standard TAS-ELISA tests. Although RBDV is common in commercial Rubus spp. worldwide, to our knowledge, this is the first report of RBDV in R. multibracteatus, and the first report of RBDV from China. The effects of this new strain of RBDV could be more or less severe, or have a different host range than previously studied strains. It is more divergent from the type isolate than any other strain that has been studied to date. Phylogenetic analysis of coat protein genes of RBDV may be useful in understanding the evolution and spread of this virus. References: (1) A. T. Jones et al. Eur. J. Plant Pathol. 106:623, 2000. (2) R. R. Martin. Can. J. Plant. Pathol. 6:264, 1984. (3) A. F. Murant. Raspberry Bushy Dwarf. Page 229 in: Virus Diseases of Small Fruits. R. H. Converse, ed. U.S. Dep. Agric. Agric. Handb. 631, 1987. (4) B. Strik and R. R. Martin. Plant Dis. 87:294, 2003.

scholarly journals First Report of Cherry virus A and Little cherry virus-1 in Poland

Plant Disease ◽  
2004 ◽  
Vol 88 (8) ◽  
pp. 909-909 ◽  
Author(s):  
B. Komorowska ◽  
M. Cieślińska
Keyword(s):  
Nucleotide Sequence ◽  
Coat Protein ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Germplasm Collection ◽  
Mottle Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Rt Pcr ◽  
First Report ◽  
Primer Sets

Cherry virus A (CVA), a member of the genus Capillovirus, has been reported in sweet cherry in Germany, Canada, and Great Britain. No data are available on the effects of CVA on fruit quality and yield of infected trees. Little cherry disease (LChD) occurs in most cherry growing areas of the world. Symptoms on sensitive cultivars include discolored fruit that remain small, pointed in shape, and tasteless. Three Closterovirus spp. associated with LChD have been described (Little cherry virus-1 [LChV-1], LChV-2, and LChV-3). Diseased local and commercial cultivars of sour cherry trees were found in a Prunus sp. germplasm collection and orchards in Poland during the 2003 growing season. The foliar symptoms included irregular, chlorotic mottling, distortion, and premature falling of leaves. Some of the diseased trees developed rosette as a result of decreased growth and shortened internodes. Severely infected branches exhibited dieback symptoms. Because the symptoms were suggestive of a possible virus infection, leaf samples were collected from 38 trees and assayed for Prune dwarf virus and Prunus necrotic ringspot virus using double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA). RNA extracted from leaves was used in a reverse transcription-polymerase chain reaction (RT-PCR) with the One-Step RT-PCR with Platinum Taq (Invitrogen Life Technologies) and primer sets specific for CVA (1), LChV-1 (3), and LChV-2 (3). The RNA samples were also tested using RT-PCR for detection of Cherry mottle leaf virus (CMLV), Cherry necrotic rusty mottle virus (CNRMV), and Cherry green ring mottle virus (CGRMV) with specific primer sets (2). Amplification of a 397-bp coat protein gene product confirmed infection of 15 trees with CVA. A 419-bp fragment corresponding to the coat protein gene of LChV-1 was amplified from cv. Gisela rootstock and local cv. WVIII/1. To confirm RT-PCR results, CVA amplification products from local cv. WX/5 and LChV-1 from cvs. Gisela and WVIII/1 were cloned in bacterial vector pCR 2.1-TOPO and then sequenced. The sequences were analyzed with the Lasergene (DNASTAR, Madison, WI) computer program. The alignment indicated that the nucleotide sequence of cv. WX/5 was closely related to the published sequences of CVA (Genbank Accession No. NC_003689) and had an 89% homology to the corresponding region. The nucleotide sequence similarity between the 419-bp fragment obtained from cvs. Gisela and WVIII/1 was 87% and 91%, respectively, compared with the reference isolate of LChV-1 (Genbank Accession No. NC_001836). The sampled trees tested negative for LChV-2, CGRMV, CMLV, and CNRMV using RT-PCR. Some trees tested positive for PNRSV and PDV. To our knowledge, this is the first report of CVA and LChV-1 in Poland. References: (1) D. James and W. Jelkmann. Acta Hortic. 472:299, 1998. (2) M. E. Rott and W. Jelkmann. Eur. J. Plant Pathol. 107:411,2001. (3) M. E. Rott and W. Jelkmann. Phytopathology. 91:61, 2001.

scholarly journals First Report of Turnip mosaic virus in Rhubarb in Alaska

Plant Disease ◽  
2005 ◽  
Vol 89 (4) ◽  
pp. 430-430 ◽  
Author(s):  
N. L. Robertson ◽  
D. C. Ianson
Keyword(s):  
Coat Protein ◽  
Host Range ◽  
Mosaic Virus ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Turnip Mosaic Virus ◽  
The Arctic ◽  
Enzyme Linked Immunosorbent Assay ◽  
Mechanical Transmission ◽  
First Report

In July 2003, noticeable red lesions were observed on rhubarb leaves (Rheum rhababarum cv. Kerwin) from a plant at the Arctic Plant Germplasm Research and Introduction Project in Palmer, AK. Extracts of leaf tissue tested positive for a potyvirus using indirect enzyme-linked immunosorbent assay (ELISA) and western blots with a monoclonal antibody specific to the potyvirus group (Agdia, Inc., Elkhart, IN). During the following growing season (June 2004), obvious chlorotic ringspots developed into red lesions on the same plant and an adjacent plant of the same cultivar. Partially purified particles that were isolated from the infected rhubarb plants were mechanically inoculated to an experimental host range (number of infected plants per total number of plants), resulting in lesions on leaves of Rheum palmatum (1 of 2) and Chenopodium amaranticolor (3 of 5) but none on C. quinoa (0 of 4). The leaves with local lesions from C. amaranticolor were ground in phosphate buffer (1 g of tissue per 10 ml of buffer), and the extract rubbed onto a set of plants resulting in lesions on R. hybridum (raponticum) (1 of 2), C. amaranticolor (1 of 4), and C. quinoa (1 of 4). The original diseased rhubarb plants and experimental symptomatic plants were confirmed to have a potyvirus using ELISA. Subsequent compound direct ELISA and western blot assays revealed that the virus reacted strongly to monoclonal or polyclonal antibodies to Turnip mosaic virus (TuMV) (Agdia, Inc.). Total RNA was extracted from leaves of the naturally infected rhubarb plants with an RNeasy Plant Mini Kit (Qiagen Sciences, Germantown, Maryland), and used in reverse-transcription-polymerase chain reaction (RT-PCR) with specific primers for TuMV (1) predicted to amplify a 1,134-bp 3′-terminal cDNA fragment encompassing the 3′-end of the nuclear inclusion protein gene (NIb), the coat protein gene, and the 3′-nontranslated region. A PCR product of approximately the expected size was obtained and then sequenced. Sequences (1,077 nt) that corresponded to the TuMV coat protein gene and 3′-terminal noncoding region were submitted to Genbank (Accession No. AY744930). Blast searches against NCBI (National Center for Biotechnology Information) contained high identities to many TuMV isolates with up to 96% (1,043 of 1,077) nucleotide identity (i.e., GenBank Accession No. AF169561). Similar high identities of up to 97% at the amino acid level occurred within the coat protein coding region (i.e., GenBank Accession No. BAC02892.1). Infected rhubarb plants were removed from the site and none of the remaining 109 plants tested positive for TuMV using ELISA. On the basis of the mechanical transmission to plant hosts, the definitive TuMV serology, and the consensus of sequenced regions with TuMV, we concluded that the causal agent of the diseased rhubarb plants was TuMV. Although TuMV has a wide plant host range occurring worldwide (2), to our knowledge, this is the first report of TuMV in rhubarb in Alaska and the first time that TuMV has been detected in Alaska. References: (1) P. Lehmann et al. Physiol. Mol. Plant Pathol. 51:195, 1997. (2) R. Provvidenti. Page 1340 in: Viruses of Plants. A. A. Brunt et al., eds. CAB International, Wallingford, UK, 1996.

scholarly journals Expression of rice gall dwarf virus outer coat protein gene (S8) in insect cells

2010 ◽  
Vol 25 (6) ◽  
pp. 401-408 ◽  
Author(s):  
Guo-cheng Fan ◽  
Fang-luan Gao ◽  
Tai-yun Wei ◽  
Mei-ying Huang ◽  
Li-yan Xie ◽  
...  
Keyword(s):  
Coat Protein ◽  
Coat Protein Gene ◽  
Insect Cells ◽  
Protein Gene ◽  
Dwarf Virus ◽  
Outer Coat ◽  
Rice Gall Dwarf Virus

Sequence and identification of the barley yellow dwarf virus coat protein gene

Virology ◽  
1988 ◽  
Vol 165 (1) ◽  
pp. 306-309 ◽  
Author(s):  
W.A. Miller ◽  
P.M. Waterhouse ◽  
A.A. Kortt ◽  
W.L. Gerlach
Keyword(s):  
Coat Protein ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Barley Yellow Dwarf Virus ◽  
Yellow Dwarf ◽  
Dwarf Virus ◽  
Barley Yellow Dwarf ◽  
Virus Coat Protein ◽  
Yellow Dwarf Virus ◽  
Virus Coat

scholarly journals Biological, Pathological, and Molecular Characteristics of a New Potyvirus, Dendrobium Chlorotic Mosaic Virus, Infecting Dendrobium Orchid

Plant Disease ◽  
2019 ◽  
Vol 103 (7) ◽  
pp. 1605-1612 ◽  
Author(s):  
Chih-Hung Huang ◽  
Chia-Hsing Tai ◽  
Ruey-Song Lin ◽  
Chung-Jan Chang ◽  
Fuh-Jyh Jan
Keyword(s):  
Amino Acid ◽  
Nucleotide Sequence ◽  
Coat Protein ◽  
Amino Acid Sequence ◽  
Mosaic Virus ◽  
Coat Protein Gene ◽  
Nucleotide Sequence Identity ◽  
Amino Acid Sequence Identity ◽  
Protein Gene ◽  
Sequence Identity

Dendrobium smillieae is one of the popular orchids in Taiwan. This report describes a new potyvirus tentatively named Dendrobium chlorotic mosaic virus (DeCMV) causing chlorotic and mosaic symptoms in D. smillieae. Enzyme-linked immunosorbent assay (ELISA) tests using six antisera against orchid-infecting viruses revealed that only a monoclonal antibody against the potyvirus group reacted positively with crude saps prepared from a symptomatic dendrobium orchid. Potyvirus-like, flexuous, filamentous particles were observed under an electron microscope, measuring approximately 700 to 800 nm in length and 11 to 12 nm in diameter. Sequence analyses revealed that DeCMV coat protein gene shared 59.6 to 66.0% nucleotide sequence identity and 57.6 to 66.0% amino acid sequence identity, whereas the DeCMV complete genome shared 54.1 to 57.3% nucleotide sequence identity and 43.7 to 49.5% amino acid sequence identity with those other known potyviruses. These similarity levels were much lower than the criteria set for species demarcation in potyviruses. Thus, DeCMV can be considered a new potyvirus. The whole DeCMV genome contains 10,041 nucleotides (GenBank accession no. MK241979) and encodes a polyprotein that is predicted to produce 10 proteins by proteolytic cleavage. In a pathogenicity test, results of inoculation assays demonstrated that DeCMV can be transmitted to dendrobium orchids by grafting and mechanical inoculation, as verified by ELISA and western blot analyses using the DeCMV polyclonal antiserum and by reverse transcription polymerase chain reaction using the coat protein gene-specific primers. The inoculated orchids developed similar chlorotic and mosaic symptoms. In conclusion, DeCMV is a novel orchid-infecting potyvirus, and this is the first report of a new potyvirus that infects dendrobium orchids in Taiwan.

scholarly journals Sequence diversity in the coat protein gene of Lettuce big-vein associated virus and Mirafiori lettuce big-vein virus infecting lettuce in Brazil

2008 ◽  
Vol 34 (2) ◽  
pp. 175-177 ◽  
Author(s):  
Márcio Martinello Sanches ◽  
Renate Krause-Sakate ◽  
Marcelo Agenor Pavan
Keyword(s):  
Amino Acid ◽  
Coat Protein ◽  
Amino Acid Sequence ◽  
Coat Protein Gene ◽  
Amino Acid Sequence Identity ◽  
Restriction Site ◽  
Protein Gene ◽  
Sequence Identity ◽  
Disease Analysis ◽  
Vein Disease

Lettuce big vein associated virus (LBVaV) and Mirafiori lettuce big vein virus (MLBVV) have been found in mixed infection in Brazil causing the lettuce big vein disease. Analysis of part of the coat protein (CP) gene of Brazilian isolates of LBVaV collected from lettuce, showed at least 93% amino acid sequence identity with other LBVaV isolates. Genetic diversity among MLBVV CP sequences was higher when compared to LBVaV CP sequences, with amino acid sequence identity ranging between 91% to 100%. Brazilian isolates of MLBVV belong to subgroup A, with one RsaI restriction site on the coat protein gene. There is no indication for a possible geografical origin for the Brazilian isolates of LBVaV and MLBVV.

Recovery of transgenic rice plants expressing the rice dwarf virus outer coat protein gene (S8)

1997 ◽  
Vol 94 (3-4) ◽  
pp. 522-527 ◽  
Author(s):  
H. H. Zheng ◽  
Y. Li ◽  
Z. H. Yu ◽  
W. Li ◽  
M. Y. Chen ◽  
...  
Keyword(s):  
Coat Protein ◽  
Transgenic Rice ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Dwarf Virus ◽  
Rice Dwarf Virus ◽  
Outer Coat ◽  
Rice Plants ◽  
Transgenic Rice Plants
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