scholarly journals First Report of Tomato chlorotic spot virus in Sweet Basil (Ocimum basilicum) and Purslane (Portulaca oleracea) in Florida

2017 ◽  
Vol 18 (2) ◽  
pp. 126-128 ◽  
Author(s):  
Richard N. Raid ◽  
Joel R. Allingham ◽  
Joseph E. Funderburk ◽  
Thomas Skarlinsky ◽  
Samuel F. Hutton ◽  
...  
Keyword(s):  
Virus Infection ◽  
Plant Species ◽  
Sequence Data ◽  
Ocimum Basilicum ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Spot Virus ◽  
First Report ◽  
Chlorotic Spot ◽  
Sweet Basil

Typical tosposvirus symptoms, lateral flow immunoassay testing, and sequence data confirm this identification, which is therefore the first report of Tomato chlorotic spot virus infection of sweet basil and purslane in Florida and of the specific plant species from any location.

scholarly journals First Report of Tomato chlorotic spot virus in Hoya wayetii and Schlumbergera truncata

2015 ◽  
Vol 16 (1) ◽  
pp. 29-30 ◽  
Author(s):  
Carlye A. Baker ◽  
Scott Adkins
Keyword(s):  
Plant Species ◽  
Vegetable Crops ◽  
Spot Virus ◽  
First Report ◽  
Chlorotic Spot ◽  
Production Space ◽  
Related Plant ◽  
Diverse Plant Species ◽  
Diverse Plant

To the best of our knowledge, this is the first report of TCSV infection of H. wayetii and S. truncata from any location, although other tospoviruses are known to infect these and related plant species. The identification of these two diverse plant species as the first reported natural ornamental hosts of TCSV has implications for TCSV epidemiology and management in ornamental and vegetable crops, which frequently share production space. Accepted by publication 15 January 2015. Published 25 February 2015.

scholarly journals First Report of Tomato chlorotic spot virus in the Non-Solanaceous Weeds Erect Spiderling (Boerhavia erecta), Asian Spiderflower (Cleome viscosa), and Sweet Chili Pepper (Capsicum chinense) in Puerto Rico

2017 ◽  
Vol 18 (1) ◽  
pp. 17-18 ◽  
Author(s):  
Consuelo Estévez de Jensen ◽  
Ismael E. Badillo-Vargas ◽  
Galen Frantz ◽  
H. Charles Mellinger ◽  
William W. Turechek ◽  
...  
Keyword(s):  
Puerto Rico ◽  
Chili Pepper ◽  
Bell Pepper ◽  
Capsicum Chinense ◽  
Spot Virus ◽  
First Report ◽  
Chlorotic Spot ◽  
New Hosts ◽  
Cleome Viscosa

Tomato chlorotic spot virus (TCSV) has recently been detected in tomato, bell pepper, jimsonweed, and lettuce in Puerto Rico. Observations of weeds and additional crops in 2015 and 2016 revealed TCSV-like symptoms. Testing of these symptomatic plants identified three new hosts of TCSV in Puerto Rico: erect spiderling (Boerhavia erecta); Asian spiderflower (Cleome viscosa); and sweet chili pepper (Capsicum chinense).

First report of Peronospora sp. on sweet basil (Ocimum basilicum) in Cuba

2010 ◽  
Vol 59 (4) ◽  
pp. 800-800 ◽  
Author(s):  
E. Martínez de la Parte ◽  
L. Pérez-Vicente ◽  
B. Bernal ◽  
D. García
Keyword(s):  
Ocimum Basilicum ◽  
First Report ◽  
Sweet Basil

scholarly journals First Report of Citrus Chlorotic Spot Virus Infecting the Succulent Plant Agave desmettiana

Plant Disease ◽  
2019 ◽  
Vol 103 (6) ◽  
pp. 1438-1438 ◽  
Author(s):  
C. Chabi-Jesus ◽  
P. L. Ramos-González ◽  
A. D. Tassi ◽  
B. M. Barguil ◽  
J. E. A. Beserra Junior ◽  
...  
Keyword(s):  
Spot Virus ◽  
First Report ◽  
Chlorotic Spot ◽  
Succulent Plant

scholarly journals First report of Tomato chlorotic spot virus on Mirabilis jalapa

2016 ◽  
Vol 11 (1) ◽  
Author(s):  
L. M. L. Duarte ◽  
A. L. R. Chaves ◽  
E. W. Kitajima ◽  
L. K. Rodrigues ◽  
R. Harakava ◽  
...  
Keyword(s):  
Spot Virus ◽  
First Report ◽  
Chlorotic Spot ◽  
Mirabilis Jalapa

scholarly journals First Report of Pepper Chlorotic Spot Virus in Bidens pilosa in Yunnan, China

Plant Disease ◽  
2019 ◽  
Vol 103 (5) ◽  
pp. 1050
Author(s):  
C. J. Huang ◽  
J. M. Zeng ◽  
H. Q. Yu ◽  
Y. Liu
Keyword(s):  
Bidens Pilosa ◽  
Spot Virus ◽  
First Report ◽  
Chlorotic Spot

scholarly journals First Report of Tomato Chlorotic Spot Virus in Soybean (Glycine max)

Plant Disease ◽  
2019 ◽  
Vol 103 (10) ◽  
pp. 2701
Author(s):  
C. Estévez de Jensen ◽  
J. E. Funderburk ◽  
T. Skarlinsky ◽  
S. Adkins
Keyword(s):  
Glycine Max ◽  
Spot Virus ◽  
First Report ◽  
Chlorotic Spot

scholarly journals First Report of Downy Mildew Caused by Peronospora belbahrii on Sweet Basil (Ocimum basilicum) in Cyprus

Plant Disease ◽  
2014 ◽  
Vol 98 (2) ◽  
pp. 283-283 ◽  
Author(s):  
L. Kanetis ◽  
A. Vasiliou ◽  
G. Neophytou ◽  
S. Samouel ◽  
D. Tsaltas
Keyword(s):  
Downy Mildew ◽  
Disease Incidence ◽  
Infected Plant ◽  
Morphological Characteristics ◽  
Ocimum Basilicum ◽  
First Report ◽  
Consensus Sequences ◽  
Sweet Basil ◽  
Plastic Bags ◽  
Necrotic Spots

Sweet basil (Ocimum basilicum L.) is an economically important annual aromatic plant, grown mostly for culinary use for both fresh and dry consumption and as a source of essential oil. In Cyprus, approximately 4 ha are grown annually, either in greenhouses as a year-round crop or in open fields from April to November, and the majority of the production is exported to the European market. During May 2012, a sweet basil cv. Genovese Gigante greenhouse operation in the area of Limassol was severely affected by a foliar disease, causing almost 100% crop losses. Within a few days, a similar, heavy disease incidence was also reported from a nearby greenhouse facility on the Genovese-type cultivars Superbo, Aroma 2, and Bonazza, as well as on Thai basil (O. basilicum var. thyrsiflorum). Successively, destructive hits of similar symptomatology have been reported from other areas and since then the disease appears to have been well-established in the country, causing major economic damages. It is also noteworthy to mention that in greenhouse infections the disease remains active even during winter, considering the mild environmental conditions and the monoculture fashion followed. Symptoms appeared on the leaves initially as interveinal, zonal, chlorotic lesions, followed by the appearance of a fuzzy, purplish sporulation on the abaxial side. Progressively, infected leaves curled and sporadic necrotic spots were evident and finally abscised. Light microscopic examination of infected samples revealed the presence of straight, hyaline sporangiophores (n = 15) typical of downy mildew, 210 to 590 μm long (mean = 350.7 μm; SD ± 117.5 μm) × 12 to 15 μm wide (mean = 13.1 μm; SD ± 1.4 μm). Sporangiophores were monopodially branched three to five times, terminating with curved branchlets bearing single sporangia at their tips. The sporangia (n = 25) were purplish-grey, ovoid to subglobose, and measured 32 to 22 μm in length (mean = 27.2 μm; SD ± 2.8 μm) and 30 to 10 μm in breadth (mean = 21.7 μm; SD ± 4.8 μm). Based on these morphological characteristics, the causal agent was identified as Peronospora belbahrii Thines (1,4). Furthermore, genomic DNA was extracted from infected plant tissue from eight different samples according to Dellaporta et al. (2). The complete ITS rDNA region was amplified and sequenced using primers ITS5 and ITS4 (3). Two of the consensus sequences were deposited in GenBank (Accession Nos. KF419289 and KF419290) and a BLAST analysis in the NCBI database revealed 99% similarity to all of the P. belbahrii sequences and other Peronospora sp. previously reported on sweet basil (Accession Nos. AY831719, DQ479408, FJ394336, and FJ436024). In a pathogenicity trial, five 40-day-old potted sweet basil plants were spray-inoculated with a sporangial suspension (1 × 105 sporangia/ml) until runoff, bagged for 24 h, and placed in a growth chamber at 18°C. Subsequently, the plastic bags were removed and the plants were kept at 22°C with a 16-h photoperiod and 80% relative humidity. Additionally, five plants were water-sprayed and served as controls. Typical downy mildew symptoms appeared 6 to 8 days after inoculation, while the uninoculated plants remained disease-free. To our knowledge, this is first report of downy mildew on sweet basil in Cyprus. References: (1) L. Belbahri et al. Mycol. Res. 109:1276, 2005. (2) S. L. Dellaporta et al. Plant Mol. Biol. Rep., 1:19, 1983. (3) G. Nagy and A. Horvat, Plant Dis. 93:1999, 2009. (4) M. Thines et al. Mycol. Res. 113:532, 2009.

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