scholarly journals Genetic variability of Haplaxius crudus, based on the 5’ region of the cytochrome c oxidase subunit I gene sheds light on epidemiology of palm lethal decline phytoplasmas

2021 ◽  
Author(s):  
Alessandra Rose Humphries ◽  
Marina Ascunce ◽  
Erica Goss ◽  
Ericka Helmick ◽  
Charles Bartlett ◽  
...  
Keyword(s):  
South Carolina ◽  
Costa Rica ◽  
Cytochrome C ◽  
Genetic Variability ◽  
Cytochrome C Oxidase ◽  
Dna Sequences ◽  
Coi Gene ◽  
Important Species ◽  
Oxidase Subunit ◽  
The Caribbean

Haplaxius crudus is an economically important species of cixiid planthopper that is widespread and abundant throughout the Caribbean basin. It is the vector of lethal yellowing (LY) and putative vector of lethal bronzing (LB), both phytoplasma diseases of palms that cause death, in Florida and the Caribbean. The primary objective of this study was to evaluate the genetic diversity of H. crudus in Florida to determine if divergent populations existed. The five prime region of the cytochrome c oxidase subunit I (COI) gene was used as the molecular marker. DNA sequences were obtained from 236 specimens collected throughout Florida, U.S.A. as well as populations from southeastern United States. Populations from Costa Rica, Colombia, and Jamaica were included to compare differences between isolated populations. In Florida, four haplotypes were discovered with 97% of individuals belonging to a single haplotype, two smaller haplotypes comprised of six and four individuals, and a single haplotype comprised of one individual. Populations from Texas and Mississippi represented distinct haplotypes whereas populations from Georgia and South Carolina were identical to the predominant haplotype in Florida. Populations from Costa Rica and Colombia were highly divergent while the population from Jamaica was 100% identical to the predominant population in Florida. These findings highlight measureable levels of genetic variability of H. crudus in Florida and the similarity to populations from Jamaica highlight the need for more robust sampling throughout the Caribbean to better understand movement and invasion potential of this species.

scholarly journals Coi Gene as a Molecular Marker of Elachista Species (Lepidoptera: Elachistidae: Elachistinae) from Different Lithuanian Populations

2009 ◽  
Vol 63 (1-2) ◽  
pp. 21-24
Author(s):  
Virginijus Sruoga ◽  
Virmantas Stunžėnas ◽  
Brigita Paulavičiūtė
Keyword(s):  
Cytochrome C ◽  
Molecular Marker ◽  
Cytochrome C Oxidase ◽  
Dna Sequences ◽  
Coi Gene ◽  
Oxidase Subunit ◽  
Gene Coi ◽  
I Gene

Coi Gene as a Molecular Marker of Elachista Species (Lepidoptera: Elachistidae: Elachistinae) from Different Lithuanian Populations We compared COI DNA sequences of three Elachista species occurring in Lithuania: Elachista maculicerusella, E. argentella, and E. pollinariella (Gelechioidea: Elachistidae: Elachistinae). Also, intraspecific differences in COI DNA between moth populations were tested. A 705 bp fragment of the 3'-end of cytochrome c oxidase subunit I gene (COI) was used. This mtDNA fragment was significantly different between all studied species. Intraspecific differences were detected only for E. maculicerusella from different Lithuanian populations. Our results support using the COI gene for identification of Elachista species and as a tool for exploring intraspecific differences between populations.

scholarly journals Molecular identification of Sarcocystis spp. in cattle: partial sequencing of Cytochrome C Oxidase subunit 1 (COI)

2019 ◽  
Vol 7 (4) ◽  
Author(s):  
Selene Rubiola ◽  
Francesco Chiesa ◽  
Stefania Zanet ◽  
Tiziana Civera
Keyword(s):  
New Species ◽  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Molecular Techniques ◽  
Coi Gene ◽  
Rrna Gene ◽  
Intermediate Hosts ◽  
Bovine Muscle ◽  
Oxidase Subunit ◽  
Sarcocystis Spp

Sarcocystis spp. are protozoan parasites with an obligatory two-host life cycle, with herbivores as intermediate hosts and carnivores as definitive hosts. Cattle are intermediate hosts for several species of Sarcocystis: indeed, in addition to S. cruzi, S. hirsuta and S. hominis, at least four new species were recently identified in bovine muscle: S. bovifelis, S. rommeli, S. bovini and S. heydorni. Since is not possible to unambiguously discriminate between S. hominis and the new species either morphologically or by the analysis of the 18S ribosomial (rRNA) gene, the aim of the present study was to use molecular techniques to discriminate cattle Sarcocystis species, taking advantage of the higher discriminative power of the Cytochrome C Oxidase subunit I mitochondrial (mtDNA COI) gene. Therefore, 119 bovine muscle samples were tested to identify S. hominis-like sarcocystis using a multiplex PCR of the 18S rRNA gene; later, positive samples were tested using a newly designed primer set for the PCR amplification of COI gene. Species identification was achieved by sequencing the amplified products: 16 sequences were confirmed to belong to S. bovifelis, while 12 sequences didn’t constitute the best BLAST match of any of the published sequences, allowing to speculate the possible presence of S. hominis. This study confirms the higher discriminatory power of COI mitochondrial gene; besides, our work provides the first report of S. bovifelis in Italy.

scholarly journals Culturing embryonic cells from the parthenogenetic clonal marble crayfish (Marmorkrebs) Procambarus virginalis Lyko, 2017 (Decapoda: Astacidea: Cambaridae)

2019 ◽  
Vol 39 (6) ◽  
pp. 758-763
Author(s):  
Heriberto Deleon ◽  
Juan Garcia ◽  
Dionn Carlo Silva ◽  
Oscar Quintanilla ◽  
Zen Faulkes ◽  
...  
Keyword(s):  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Early Stage ◽  
Model Organism ◽  
Coi Gene ◽  
Embryonic Cells ◽  
Oxidase Subunit ◽  
Marbled Crayfish

Abstract The parthenogenetic marbled crayfish, or Marmorkrebs (Procambarus virginalis Lyko 2017), is an emerging model organism. We describe a method to isolate cells from early-stage embryos and culture them in vitro. The identity of the cells was confirmed by sequencing the cytochrome c oxidase subunit I (COI) gene. This technique can be applied for use in the manipulation of embryonic parthenogenetic crayfish cells.

Two new feather mites (Acari: Analgoidea) isolated from the grey-headed woodpecker, Picus canus (Piciformes: Picidae) in Korea

2019 ◽  
Vol 24 (11) ◽  
pp. 2167-2183
Author(s):  
Yeong-deok Han ◽  
Sergey V. Mironov ◽  
Gi-sik Min
Keyword(s):  
New Species ◽  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Coi Gene ◽  
Mitochondrial Cytochrome ◽  
Dna Barcodes ◽  
Feather Mites ◽  
Oxidase Subunit ◽  
Two New Species ◽  
First Time

Two new species of feather mites from the superfamily Analgoidea are described from the grey-headed woodpecker, Picus canus, in Korea: Neopteronyssus koreanus sp. nov. (Pteronyssidae) and Proterothrix picinus sp. nov. (Proctophyllodidae: Pterodectinae). Feather mites of the genera Neopteronyssus Mironov, 2002 and Proterothrix Gaud, 1968 are described for the first time in Korea. Morphological descriptions of both new species are complemented with partial sequences of their mitochondrial cytochrome c oxidase subunit I (COI) gene as DNA barcodes.

Comparative study on DNA sequences of ribosomal DNA and cytochrome c oxidase subunit 1 of mitochondrial DNA among five species of gnathostomes

2006 ◽  
Vol 80 (1) ◽  
pp. 7-13 ◽  
Author(s):  
K. Ando ◽  
M. Tsunemori ◽  
H. Akahane ◽  
S. Tesana ◽  
H. Hasegawa ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Ribosomal Dna ◽  
Dna Sequences ◽  
Nucleotide Sequences ◽  
Internal Transcribed Spacer Region ◽  
Cycle Sequencing ◽  
Oxidase Subunit ◽  
Species Being

AbstractThe nucleotide sequences of partial 18S, complete internal transcribed spacer region 1 (ITS1), complete 5.8S, complete ITS2 and partial 28S of ribosomal DNA (rDNA) and cytochrome c oxidase subunit 1 of mitochondrial DNA (MCOI) from five species of gnathostomes (G. spinigerum, G. doloresi, G. nipponicum, G. hispidum and G. binucleatum with the former four species being distributed in Japan and Asia) that cause human gnathostomiasis were compared by direct polymerase chain reaction cycle-sequencing. The nucleotide sequences of each region of the18S (613 bp), 5.8S (158 bp) and 28S (598 bp) rDNA from the five species were almost identical. The ITS1 region was different in length for the five species. The nucleotide sequences of each region of ITS2 and partial MCO1 regions were different among the five species. Therefore, these two regions can be used as genetic markers for identification of worms.

scholarly journals Biometric and genetic differences in kelabau (Osteochilus spp.) as revealed using cytochrome c oxidase subunit 1

F1000Research ◽  
2020 ◽  
Vol 8 ◽  
pp. 177
Author(s):  
Nur Asiah ◽  
Junianto Junianto ◽  
Ayi Yustiati ◽  
Sukendi Sukendi ◽  
Melta Rini Fahmi ◽  
...  
Keyword(s):  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Dna Sequences ◽  
Phylogenetic Trees ◽  
Similarity Index ◽  
Research Data ◽  
Oxidase Subunit ◽  
Nucleotide Distance ◽  
Fish Samples

Background: Kelabau (Osteochilus spp.) is a freshwater fish commonly found in the rivers of Riau, Indonesia. Researchers believe that these are Osteochilus kelabau; however, accurate taxonomic determination of these fish in Riau waters has not been made. The purpose of this study was to facilitate the identification of the kelabau based on its morphology and genetics using biometric and cytochrome c oxidase subunit 1 (CO1) analyses, respectively. Methods: Fish samples were collected from the Siak, Kampar and Rokan rivers in Riau Province, Indonesia. The DNA of 90 fish was extracted from the caudal fins using a DNA extraction kit, after which it was amplified using primers Fish-F1 and Fish-R1. Sequencing was conducted by Applied Biosystems Macrogen Korea, and the DNA sequences were then edited and aligned using MEGA v. 7. All samples were BLAST-searched for identification using the National Center for Biotechnology Information and BOLD System. Phylogenetic trees were constructed, and the similarity index was calculated using accession numbers AP011385.1 and KC631202.1 in GenBank. Results: Analysis of the consensus barcode sequence for 86 species revealed a high percentage of barcode matches (96%–97% in GenBank and 96.6%–96.76% in the BOLD System). The nucleotide distance between groups of kelabau from the different rivers based on the Kimura 2-parameter model gave the following results: 0.05% between groups from the Siak and Kampar rivers, 0.09% between those from the Siak and Rokan rivers and 0.05% between those from the Kampar and Rokan rivers. The nucleotide distance between the groups in the Siak (0.09%), Kampar (0.00%) and Rokan (0.10%) Rivers indicated that the kelabau in those rivers were related to each other. Conclusions: Based on the results of the research data using CO1 and biometric analyses, the kelabau were confirmed to be O. melanopleurus.

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