scholarly journals Determination of Genes Involved in the Early Process of Embryonic Implantation in Rhesus Monkey (Macaca mulatta) by Suppression Subtractive Hybridization1

2004 ◽  
Vol 70 (5) ◽  
pp. 1365-1373 ◽  
Author(s):  
Xiao-Yang Sun ◽  
Fei-Xue Li ◽  
Jun Li ◽  
Yin-Fei Tan ◽  
Yun-Shang Piao ◽  
...  
Keyword(s):  
2018 ◽  
Vol 50 (8) ◽  
pp. 628-635 ◽  
Author(s):  
Uros Midic ◽  
Catherine A. VandeVoort ◽  
Keith E. Latham

To account for sex as a biological variable, it is sometimes necessary to identify the sex of an embryo or embryonic cell that was used to generate libraries for RNA sequencing, without the sex being known a priori. The preferred approach for this would take advantage of the mRNA data, rather than relying on other methods that require separation and analysis of genomic DNA or diversion of limiting RNA for other assays. We describe here a method that has been optimized for this purpose in samples of rhesus monkey and mouse embryos. This method is broadly applicable to any species for which a sufficiently well characterized genome and knowledge of polymorphisms are available, and for embryos that are transcriptionally active and expressing their genome.


1980 ◽  
Vol 84 (3) ◽  
pp. 489-494 ◽  
Author(s):  
G. JENKIN ◽  
M. D. MITCHELL ◽  
P. HOPKINS ◽  
C. D. MATTHEWS ◽  
G. D. THORBURN

A radioimmunoassay for the determination of concentrations of melatonin in the plasma of rhesus monkeys has been developed. Antiserum for the assay was raised against N-acetyl serotonin and there was a 100% cross-reaction with melatonin. Cross-reactivity with closely related indoles, precursors and metabolites was less than or equal to 1%. The lower limit of sensitivity of the assay was 4 pg/tube. The assay has been used for the investigation of diurnal variations and cyclical changes in melatonin concentrations in peripheral plasma of the rhesus monkey. The concentrations of melatonin ranged between 26·6 and 85·3 pg/ml during sampling for 24 h.There was a distinct diurnal variation in the concentration of melatonin in plasma. The concentration during darkness (61·0 ± 7·1 (s.e.m.) pg/ml) was greater (P <0·01) than that during illumination (40·1 ± 6·1 pg/ml). There were no significant differences in the concentration of melatonin in plasma at any time during the 28 day menstrual cycle of the rhesus monkey.


1976 ◽  
Vol 19 (3) ◽  
pp. 246-251 ◽  
Author(s):  
Helen H. Molinari ◽  
Andrew J. Rózsa ◽  
Dan R. Kenshalo

2021 ◽  
Vol 59 (1) ◽  
pp. 175-182
Author(s):  
Dong-hong Tang ◽  
Chen-yun Wang ◽  
Xi Huang ◽  
Hong-kun Yi ◽  
Zhe-li Li ◽  
...  

1978 ◽  
Vol 235 (1) ◽  
pp. R29-R34 ◽  
Author(s):  
P. R. McHugh ◽  
T. H. Moran

In seven male monkeys, Macaca mulatta, the infusion of nutrients into the stomach just prior to or 20 h before a 4-h feeding period reduced the feeding by an amount comparable to the calories infused. Pure carbohydrates, fat, protein, and mixtures were employed as infusions and given in a random fashion over a caloric range of 75-300 kcal. In a second series of experiments, monkeys were partially fasted on 1 day and in this way deprived of 75, 150, 300, or 450 kcal. On successive days, they overate to compensate for this deprivation. The smaller deprivations (75 and 150 kcal) were corrected on the first recovery day. The 300-kcal deprivation required 2 days to be corrected while the 450-kcal deficit was only partially restored. These experiments demonstrate the capacities of the monkey to respond with precision to caloric supply and deprivation so as to maintain a constant caloric intake.


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