CONCENTRATIONS OF MELATONIN IN THE PLASMA OF THE RHESUS MONKEY (MACACA MULATTA)

1980 ◽  
Vol 84 (3) ◽  
pp. 489-494 ◽  
Author(s):  
G. JENKIN ◽  
M. D. MITCHELL ◽  
P. HOPKINS ◽  
C. D. MATTHEWS ◽  
G. D. THORBURN
Keyword(s):  
Menstrual Cycle ◽  
Rhesus Monkey ◽  
Diurnal Variation ◽  
Lower Limit ◽  
Macaca Mulatta ◽  
Rhesus Monkeys ◽  
Cross Reactivity ◽  
Diurnal Variations ◽  
Peripheral Plasma

A radioimmunoassay for the determination of concentrations of melatonin in the plasma of rhesus monkeys has been developed. Antiserum for the assay was raised against N-acetyl serotonin and there was a 100% cross-reaction with melatonin. Cross-reactivity with closely related indoles, precursors and metabolites was less than or equal to 1%. The lower limit of sensitivity of the assay was 4 pg/tube. The assay has been used for the investigation of diurnal variations and cyclical changes in melatonin concentrations in peripheral plasma of the rhesus monkey. The concentrations of melatonin ranged between 26·6 and 85·3 pg/ml during sampling for 24 h.There was a distinct diurnal variation in the concentration of melatonin in plasma. The concentration during darkness (61·0 ± 7·1 (s.e.m.) pg/ml) was greater (P <0·01) than that during illumination (40·1 ± 6·1 pg/ml). There were no significant differences in the concentration of melatonin in plasma at any time during the 28 day menstrual cycle of the rhesus monkey.

THE NUMBER OF OOCYTES IN THE MATURE RHESUS MONKEY (MACACA MULATTA)

1951 ◽  
Vol 7 (2) ◽  
pp. 194-202 ◽  
Author(s):  
S. H. GREEN ◽  
S. ZUCKERMAN
Keyword(s):  
Statistical Analysis ◽  
Menstrual Cycle ◽  
Rhesus Monkey ◽  
Macaca Mulatta ◽  
Rhesus Monkeys ◽  
Significant Difference ◽  
Average Total Number ◽  
Sexually Mature

Estimates have been made of the total number of oocytes in ovaries removed on different days of the menstrual cycle from twelve sexually mature rhesus monkeys. They have been analysed on two assumptions: (a) that no relation exists over the range of age studied between age and the number of oocytes present; and (b) that such a relation exists. The data were grouped in the following three temporal phases of the menstrual cycle: days 1–9, days 10–18, days 19–27. Statistical analysis failed to reveal any significant difference between the average total number of oocytes present at the beginning, the middle, or the end of the menstrual cycle.

INFLUENCE OF OOPHORECTOMY, LUTEECTOMY, FOETAL DEATH AND DEXAMETHASONE ON THE PERIPHERAL PLASMA LEVELS OF OESTROGENS AND PROGESTERONE IN THE PREGNANT MACACA MULATTA

1974 ◽  
Vol 75 (3) ◽  
pp. 601-616 ◽  
Author(s):  
W. T. K. Bosu ◽  
E. D. B. Johansson ◽  
C. Gemzell
Keyword(s):  
Rhesus Monkey ◽  
Macaca Mulatta ◽  
Plasma Levels ◽  
Rhesus Monkeys ◽  
Deleterious Effect ◽  
Maternal Plasma ◽  
Relative Influence ◽  
Foetal Death ◽  
Bilateral Oophorectomy ◽  
Peripheral Plasma

ABSTRACT The influence of bilateral oophorectomy, luteectomy, dexamethasone injections and foetal death on the peripheral plasma levels of oestrogens1) and progesterone was studied in pregnant rhesus monkeys. After bilateral oophorectomy and luteectomy, pregnancy proceeded normally and the plasma levels of oestrogens and progesterone attained levels similar to those in intact monkeys, but the progesterone levels after parturition were comparatively lower. Dexamethasone injections into pregnant monkeys had no deleterious effect on the outcome of gestation. Abrupt and dramatic decreases in total oestrogens, oestrone and oestradiol levels occurred during dexamethasone injections into intact and oophorectomized pregnant monkeys but no significant changes in progesterone levels in plasma were observed. Spontaneous intra-uterine foetal death was accompanied by decrease in oestrogens, but no changes in the plasma levels of progesterone. In experimentally induced foetal death, the oestrogen and progesterone levels in plasma decreased prior to abortion. These data demonstrate the relative influence of the ovaries, adrenals and the placenta on the maternal plasma levels of oestrogens and progesterone in the pregnant rhesus monkey.

PROSTAGLANDIN E, THROMBOXANE B2 AND 6-OXO-PROSTAGLANDIN F1α IN AMNIOTIC FLUID AND MATERNAL PLASMA OF RHESUS MONKEYS (MACACA MULATTA) DURING THE LATTER THIRD OF GESTATION

1979 ◽  
Vol 81 (3) ◽  
pp. 345-349 ◽  
Author(s):  
J. S. ROBINSON ◽  
R. NATALE ◽  
L. CLOVER ◽  
M. D. MITCHELL
Keyword(s):  
Rhesus Monkey ◽  
Amniotic Fluid ◽  
Macaca Mulatta ◽  
Rhesus Monkeys ◽  
Maternal Plasma ◽  
Thromboxane B2 ◽  
Prostaglandin E ◽  
Peripheral Plasma ◽  
Prostaglandin F ◽  
Serial Samples

The concentrations of prostaglandin E (PGE), thromboxane B2 (TXB2) and 6-oxo-prostaglandin F1α (6-oxo-PGF1α) were measured by radioimmunoassay in serial samples of amniotic fluid and maternal peripheral plasma in the latter third of pregnancy in rhesus monkeys (Macaca mulatta). The samples were collected under ketamine-induced anaesthesia. The concentration of PGE was undetectable in amniotic fluid until a few days before delivery when a large increase was observed in three of the five animals. There were small increases of TXB2 and 6-oxo-PGF1α in amniotic fluid before delivery. In maternal plasma the concentrations of PGE, TXB2 and 6-oxo-PGF1α were generally higher and more variable than in amniotic fluid and did not increase with advancing gestation. It is suggested that increased production of primary prostaglandins occurs before, and is involved in, the onset of parturition in the rhesus monkey.

FURTHER OBSERVATIONS ON OOCYTE NUMBERS IN MATURE RHESUS MONKEYS (MACACA MULATTA)

1954 ◽  
Vol 10 (3) ◽  
pp. 284-290 ◽  
Author(s):  
S. H. GREEN ◽  
S. ZUCKERMAN
Keyword(s):  
Body Weight ◽  
Menstrual Cycle ◽  
Macaca Mulatta ◽  
Rhesus Monkeys ◽  
Stages Of Development ◽  
The Right

SUMMARY An analysis of oocyte numbers in forty-two rhesus monkeys whose ovaries were removed on different days of the menstrual cycle showed (1) that the right and left ovaries usually contain about the same number of oocytes; (2) that oocyte numbers vary inversely with body weight; (3) that there are no significant differences in the total number of oocytes in the early, middle and last thirds of the cycle; and (4) that no constant relation exists between the proportion of follicles in different stages of development.

An enzyme-linked immunosorbent assay to study human relaxin in human pregnancy and in pregnant rhesus monkeys

1989 ◽  
Vol 120 (3) ◽  
pp. 449-457 ◽  
Author(s):  
C. Lucas ◽  
L. N. Bald ◽  
M. C. Martin ◽  
R. B. Jaffe ◽  
D. W. Drolet ◽  
...  
Keyword(s):  
Rhesus Monkey ◽  
Pregnant Women ◽  
Human Insulin ◽  
Rhesus Monkeys ◽  
Cross Reactivity ◽  
Enzyme Linked Immunosorbent Assay ◽  
Corpora Lutea ◽  
Synthetic Analogue ◽  
Human Pregnancy

ABSTRACT A sensitive and specific double-antibody enzyme-linked immunoassay, using a synthetic analogue of human relaxin for standard and immunogen, was developed for the measurement of human relaxin (hRLX) in serum and plasma. No cross-reactivity was observed for human insulin, human insulin-like growth factor-I, hGH, human chorionic gonadotropin, hFSH, hLH or human prolactin. The assay was used to monitor RLX concentrations in samples from men, non-pregnant and pregnant women, and in pregnant rhesus monkeys infused with hRLX. RLX was not detected in serum from men nor from non-pregnant women, while a concentration of 600 ng/l was measured in pooled sera from two pregnant women (pregnancies achieved by in-vitro fertilization). Immunoreactive RLX (1·1 μg/g) was found in human corpora lutea taken from ectopic pregnancies at 7 weeks. In an experiment with a pregnant rhesus monkey infused with human RLX analogue, less than 1·5% of the maternal concentration was measured in the fetal circulation. Even though preliminary, these data suggest a low level of transfer of human analogue relaxin across the placenta in a rhesus monkey. Further studies of the physiology of RLX in human pregnancy will be facilitated by the availability of this immunoassay. Journal of Endocrinology (1989) 120, 449–457

scholarly journals Selection for Urease Activity duringHelicobacter pylori Infection of Rhesus Macaques (Macaca mulatta)

2001 ◽  
Vol 69 (5) ◽  
pp. 3519-3522 ◽  
Author(s):  
Lori M. Hansen ◽  
Jay V. Solnick
Keyword(s):  
Helicobacter Pylori ◽  
Rhesus Monkey ◽  
Macaca Mulatta ◽  
Rhesus Monkeys ◽  
Urease Activity ◽  
Rhesus Macaques ◽  
Selection For ◽  
H Pylori ◽  
Selection Of

ABSTRACT Helicobacter pylori strain J166 recovered from experimentally inoculated rhesus monkeys had up to a 250-fold-increased urease activity over that before inoculation. This was found to result from the selection of urease positive J166 clones from a heterogenous inoculum, which was predominantly urease negative due to a 1-bp insertion in the ureA gene. These results confirm the importance of urease for H. pylori colonization. Strain J166 is particularly well adapted to the rhesus monkey, since it colonized preferentially despite the fact that less than 0.1% of the inoculum was urease positive.

scholarly journals The menstrual cycle of the primates - X—The oestrone threshold of the uterus of the rhesus monkey

1937 ◽  
Vol 123 (833) ◽  
pp. 441-456 ◽  
Keyword(s):  
Experimental Study ◽  
Menstrual Cycle ◽  
Rhesus Monkey ◽  
Rhesus Monkeys ◽  
Ovarian Follicles ◽  
Clinical Findings ◽  
Uterine Bleeding ◽  
Corpora Lutea ◽  
Detailed Knowledge

Uterine bleeding occurs in spayed rhesus monkeys a few days after the cessation of a series of oestrin injections (Allen 1927). The fact that bleeding also follows either the removal of ovaries which do not contain functional corpora lutea or injury to large ovarian follicles (Allen 1927; van Wagenen and Aberle 1931) is now related to the occurrence of post-oestrin bleeding, and these various observations, together with parallel and in some cases long-established clinical findings, have been made the basis of what is known as the oestrin-withdrawal theory of menstruation. The experimental study of the menstrual cycle necessarily requires detailed knowledge of the varying reactions of the primate uterus to oestrin. The available observations on this question are very few, and for that reason the following study was undertaken.

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