scholarly journals In Vitro Production of Estradiol by Bovine Granulosa Cells: Evaluation of Culture Condition, Stage of Follicular Development, and Location of Cells Within Follicles1

1994 ◽  
Vol 51 (2) ◽  
pp. 273-282 ◽  
Author(s):  
A. J. Roberts ◽  
S. E. Echternkamp
Keyword(s):  
Granulosa Cells ◽  
Culture Condition ◽  
Follicular Development ◽  
In Vitro Production ◽  
Vitro Production

In vitro production of progesterone and estradiol by rat granulosa cells regulated by cabergoline and prolactin

1998 ◽  
Vol 24 (2) ◽  
pp. 195-203 ◽  
Author(s):  
H. Negishi ◽  
I. Furuta ◽  
K. Ushigoe ◽  
S. Fujimoto ◽  
S. S. Koide
Keyword(s):  
Granulosa Cells ◽  
In Vitro Production ◽  
Vitro Production

In vitro production of estradiol by ovarian granulosa cells in a case of McCune-Albright syndrome

1991 ◽  
Vol 14 (4) ◽  
pp. 311-315 ◽  
Author(s):  
C. Manna ◽  
O. Epifano ◽  
M. Riminucci ◽  
L. Baschieri ◽  
M. Stefanini ◽  
...  
Keyword(s):  
Granulosa Cells ◽  
In Vitro Production ◽  
Mccune Albright Syndrome ◽  
Ovarian Granulosa Cells ◽  
Albright Syndrome ◽  
Vitro Production ◽  
Mccune Albright

In vitro production of plasminogen activator by human granulosa cells.

1994 ◽  
Vol 78 (1) ◽  
pp. 174-179
Author(s):  
O Epifano ◽  
M Riminucci ◽  
C Manna ◽  
R Apa ◽  
E Greco ◽  
...  
Keyword(s):  
Plasminogen Activator ◽  
Granulosa Cells ◽  
In Vitro Production ◽  
Human Granulosa Cells ◽  
Vitro Production

scholarly journals Collection grading and evaluation of cumulus-oocyte-complexes for in vitro maturation in sheep

1970 ◽  
Vol 28 (1) ◽  
pp. 31-38
Author(s):  
MNS Talukder ◽  
A Iqbal ◽  
MAMY Khandoker ◽  
MZ Alam
Keyword(s):  
Corpus Luteum ◽  
Culture Condition ◽  
In Vitro Maturation ◽  
Type I ◽  
Type Ii ◽  
In Vitro Production ◽  
Length Width ◽  
Left And Right ◽  
Vitro Production

An alternative to superovulation is in-vitro production (IVP) of embryos where the efficient collection and grading of oocytes is important. Ovaries from an abattoir were collected and categorized as type I with no corpus luteum (CL), and type II with CL. The length, width and weight of type I and type II ovary were 1.4 ± 0.03 and 1.5 ± 0.08 cm; 0.8 ± 0.04 and 1.0 ± 0.07 cm; 0.6 ± 0.07 and 0.7 ± 0.04 gm, respectively, each significantly (P<0.05) higher in type II ovaries. A total of 80 and 78 follicles were observed and 60 and 61 follicles aspirated from left and right ovaries, respectively, from each of 25 ovaries. Out of 133 follicles 100 were aspirated from 40 type-I ovaries, and 21 aspirated from 10 type-II ovaries. The differences in the number of normal, abnormal and total cumulus-oocytecomplex (COCs) per ovary between left and right ovaries were not significant (P>0.05). The number of normal (1.9 ± 0.11) and total (2.5 ± 0.14) COCs per ovary were significantly (P<0.05) higher in ovaries without than in those with CL (1.2 ± 0.36 and 2.0 ± 0.30, respectively). But the number (0.80 ± 0.13) of abnormal COCs per ovary was significantly (P<0.05) higher in ovaries with CL than in those without (0.7 ± 0.09). Significantly (P<0.05) higher percentage of COCs expansion was grade A (6.9 ± 2.05) than grade B (53.1 ± 1.27) COCs. It is suggested that type I (without CL) ovaries and follicles of 2-6 mm diameter are suitable to collect good quality COCs for in-vitro maturation (IVM) of oocytes and the culture condition for IVM of sheep COCs are reported. DOI: http://dx.doi.org/10.3329/bvet.v28i1.8811   Bangl. vet. 2011. Vol. 28, No. 1, 31-38

scholarly journals Hormonal protocols for in vitro production of Zebu and taurine embryos

2014 ◽  
Vol 49 (10) ◽  
pp. 813-817 ◽  
Author(s):  
Carlos Antônio de Carvalho Fernandes ◽  
Tatimara Maria Miyauchi ◽  
Ana Cristina Silva de Figueiredo ◽  
Miller Pereira Palhão ◽  
Fabiana Cristina Varago ◽  
...  
Keyword(s):  
Bos Indicus ◽  
Follicular Development ◽  
Cold Season ◽  
Control Group ◽  
In Vitro Production ◽  
Follicular Wave ◽  
Different Seasons ◽  
Pre Treatment ◽  
Vitro Production

The objective of this work was to evaluate the effects of hormonal synchronization protocols, associated or not with follicular development stimulation, on the recovery of oocytes and on in vitro production of Bos indicus and B. taurus embryos, in different seasons. Ultrasound-guided follicular aspirations (n=237) were performed without pre-treatment (G1, control group) and after follicular wave synchronization (G2), or after follicular wave synchronization and follicle growth induction (G3). Bos indicus produced more oocytes and embryos than B. taurus (18.7±0.9 vs. 11.9±0.6 oocytes and 4.8±0.3 vs. 2.1±0.2 embryos). On average, oocyte and embryo yields were higher in G3 than in G2, and both were greater than in G1, which lead to a higher conversion of oocytes to embryos in these treatments. The hot or the cold season did not affect the B. indicus outcomes, whereas, in B. taurus, both oocyte recovery and embryo production were higher in the cold season. Follicular wave synchronization improves ovum pick-up and in vitro production of embryos in both cattle subspecies evaluated.

scholarly journals In Vitro Production Of Goat Embryos In Bangladesh

1970 ◽  
Vol 37 (1) ◽  
pp. 1-9
Author(s):  
A Mondal ◽  
MAMY Khandoker ◽  
MA Mondal ◽  
AHMS Rahman ◽  
AS Apu ◽  
...  
Keyword(s):  
Corpus Luteum ◽  
Culture Condition ◽  
In Vitro Maturation ◽  
Calf Serum ◽  
Type I ◽  
Type Ii ◽  
In Vitro Production ◽  
Vitro Fertilization ◽  
Vitro Production

The present study was undertaken to collect the quality cumulus-oocyte-complexes (COCs) from ovaries of goat from slaughterhouse by aspiration to establish the suitable culture condition for in vitro maturation (IVM), in vitro fertilization (IVF) and in vitro culture (IVC). Follicular COCs were collected from follicles of 2-6 mm diameter, categorized by microscopic observation and cultured for 22 h in TCM-199 medium supplemented with 5% fetal calf serum (FCS) to determine the success rate of in vitro maturation in a condition of 5% CO2 in air at 38.5°C. The collected ovaries were classified as type-I (corpus luteum absent) and type-II (corpus luteum present). The average numbers of follicles aspirated per ovary were 3.15 and 2.57 in type-I and type-II, respectively. The collected COCs were classified into normal COCs (grade A and B) and abnormal COCs (grade C and D). The number of normal and abnormal COCs collected from two type of ovaries were significantly (P<0.01) differed. Average number of normal COCs per ovary obtained from type-I (1.30) was significantly (P<0.01) higher than that of type-II (0.68). Within the normal COCs significantly (P<0.01) higher maturation was obtained in grade A COCs (71.70%) than that of grade B (51.52%). The matured COCs were cultured for 5 h with fresh buck semen in Brackett and Oliphant (BO) medium and assumed that the COCs were fertilized successfully. In progress, IVC was practiced in TCM-199 supplemented with FCS and bovine serum albumen (BSA) at 38.5°C with 5% CO2 for 6-7 days. The rate of development to compact morula was found significantly (P<0.01) higher in grade A (25.64%) compared to grade B COCs (6.89%) and similar trend of blastocyst was found in grade A COCs (12.82%) than that of grade of B (3.45%). The results suggested that culture condition for IVM, IVF and IVC was found optimum and grade A COCs might be suitable for in vitro production (IVP) of goat embryos.DOI: http://dx.doi.org/10.3329/bjas.v37i1.9859 BJAS 2008; 37(1): 1-9

THE IN VITRO PRODUCTION OF ANDROGENS BY FOLLICULAR TISSUE OF RABBITS

1964 ◽  
Vol 47 (2) ◽  
pp. 306-313 ◽  
Author(s):  
Denis Gospodarowicz
Keyword(s):  
In Vitro Production ◽  
Vitro Production

ABSTRACT Incubation in vitro of rabbit follicles in separate experiments with dehydroepiandrosterone-14C (DHEA-14C), progesterone-14C and pregnenolone-3H in the presence of FSH gave the following results: 39 % of the radioactivity of DHEA-14C is converted to androstenedione and testosterone, while only 3 % of the radioactivity of either progesterone-14C or pregnenolone-3H is found in the androgen fraction. From the ratio of testosterone to androstenedione formed from the three precursors, the results are interpreted to mean that DHEA and pregnenolone, and not progesterone, are precursors of androgens in the follicle.

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