In vitro production of plasminogen activator by human granulosa cells

1994 ◽  
Vol 78 (1) ◽  
pp. 174-179 ◽  
Author(s):  
O. Epifano
Keyword(s):  
Plasminogen Activator ◽  
Granulosa Cells ◽  
In Vitro Production ◽  
Human Granulosa Cells ◽  
Vitro Production

In vitro production of plasminogen activator by human granulosa cells.

1994 ◽  
Vol 78 (1) ◽  
pp. 174-179
Author(s):  
O Epifano ◽  
M Riminucci ◽  
C Manna ◽  
R Apa ◽  
E Greco ◽  
...  
Keyword(s):  
Plasminogen Activator ◽  
Granulosa Cells ◽  
In Vitro Production ◽  
Human Granulosa Cells ◽  
Vitro Production

In vitro production of progesterone and estradiol by rat granulosa cells regulated by cabergoline and prolactin

1998 ◽  
Vol 24 (2) ◽  
pp. 195-203 ◽  
Author(s):  
H. Negishi ◽  
I. Furuta ◽  
K. Ushigoe ◽  
S. Fujimoto ◽  
S. S. Koide
Keyword(s):  
Granulosa Cells ◽  
In Vitro Production ◽  
Vitro Production

In vitro production of estradiol by ovarian granulosa cells in a case of McCune-Albright syndrome

1991 ◽  
Vol 14 (4) ◽  
pp. 311-315 ◽  
Author(s):  
C. Manna ◽  
O. Epifano ◽  
M. Riminucci ◽  
L. Baschieri ◽  
M. Stefanini ◽  
...  
Keyword(s):  
Granulosa Cells ◽  
In Vitro Production ◽  
Mccune Albright Syndrome ◽  
Ovarian Granulosa Cells ◽  
Albright Syndrome ◽  
Vitro Production ◽  
Mccune Albright

THE IN VITRO PRODUCTION OF ANDROGENS BY FOLLICULAR TISSUE OF RABBITS

1964 ◽  
Vol 47 (2) ◽  
pp. 306-313 ◽  
Author(s):  
Denis Gospodarowicz
Keyword(s):  
In Vitro Production ◽  
Vitro Production

ABSTRACT Incubation in vitro of rabbit follicles in separate experiments with dehydroepiandrosterone-14C (DHEA-14C), progesterone-14C and pregnenolone-3H in the presence of FSH gave the following results: 39 % of the radioactivity of DHEA-14C is converted to androstenedione and testosterone, while only 3 % of the radioactivity of either progesterone-14C or pregnenolone-3H is found in the androgen fraction. From the ratio of testosterone to androstenedione formed from the three precursors, the results are interpreted to mean that DHEA and pregnenolone, and not progesterone, are precursors of androgens in the follicle.

Regulatory effect of vasopressin and its analogs on the steroidogenesis of human granulosa cells (GC) in vitro

1989 ◽  
Vol 120 (3_Suppl) ◽  
pp. S183-S185
Author(s):  
H. MUELLER ◽  
T. RABE ◽  
B. HAUFF ◽  
L. KIESEL ◽  
B. RUNNEBAUM
Keyword(s):  
Granulosa Cells ◽  
Regulatory Effect ◽  
Human Granulosa Cells

Oxygenation of 18-hydroxycorticosterone as the final reaction for aldosterone biosynthesis

1984 ◽  
Vol 107 (3) ◽  
pp. 395-400 ◽  
Author(s):  
Itaru Kojima ◽  
Etsuro Ogata ◽  
Hiroshi Inano ◽  
Bun-ichi Tamaoki
Keyword(s):  
Carbon Monoxide ◽  
Hydroxysteroid Dehydrogenase ◽  
Dependent Manner ◽  
In Vitro Production ◽  
Mitochondrial Preparation ◽  
Final Reaction ◽  
Vitro Production ◽  
Dose Dependent ◽  
Cytochrome P 450

Abstract. Incubation of 18-hydroxycorticosterone with the sonicated mitochondrial preparation of bovine adrenal glomerulosa tissue leads to the production of aldosterone, as measured by radioimmunoassay. The in vitro production of aldosterone from 18-hydroxycorticosterone requires both molecular oxygen and NADPH, and is inhibited by carbon monoxide. Cytochrome P-450 inhibitors such as metyrapone, SU 8000. SU 10603, SKF 525A, amphenone B and spironolactone decrease the biosynthesis of aldosterone from 18-hydroxycorticosterone. These results support the conclusion that the final reaction in aldosterone synthesis from 18-hydroxycorticosterone is catalyzed by an oxygenase, but not by 18-hydroxysteroid dehydrogenase. By the same preparation, the production of [3H]aldosterone but not [3H]18-hydroxycorticosterone from [1,2-3H ]corticosterone is decreased in a dose-dependent manner by addition of non-radioactive 18-hydroxycorticosterone.

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