Investigation of the Directionality and Kinetics of DNA Transfer by a Bacterial Type IV Secretion System

2020 ◽  
Vol 34 (S1) ◽  
pp. 1-1
Author(s):  
Olukemi P. Akinleye ◽  
Betelhem B. Gemechu ◽  
Sabrina Ali ◽  
Melanie B. Berkmen
Keyword(s):  
Secretion System ◽  
Type Iv Secretion ◽  
Dna Transfer ◽  
Type Iv Secretion System ◽  
Type Iv ◽  
Kinetics Of ◽  
Bacterial Type

scholarly journals Exploring Directionality and Kinetics of a Bacterial Type IV Secretion System

2021 ◽  
Vol 35 (S1) ◽  
Author(s):  
Olukemi Akinleye ◽  
Betelhem Gemechu ◽  
Sabrina Ali ◽  
Melanie Berkmen
Keyword(s):  
Secretion System ◽  
Type Iv Secretion ◽  
Type Iv Secretion System ◽  
Type Iv ◽  
Kinetics Of ◽  
Bacterial Type

scholarly journals Critical Role of ASC Inflammasomes and Bacterial Type IV Secretion System in Caspase-1 Activation and Host Innate Resistance toBrucella abortusInfection

2013 ◽  
Vol 190 (7) ◽  
pp. 3629-3638 ◽  
Author(s):  
Marco Tulio R. Gomes ◽  
Priscila C. Campos ◽  
Fernanda S. Oliveira ◽  
Patricia P. Corsetti ◽  
Karina R. Bortoluci ◽  
...  
Keyword(s):  
Critical Role ◽  
Secretion System ◽  
Type Iv Secretion ◽  
Type Iv Secretion System ◽  
Innate Resistance ◽  
Caspase 1 ◽  
Type Iv ◽  
Bacterial Type

scholarly journals Additional conjugation systems for inter-domain plasmid transfer to the diatom Phaeodactylum tricornutum

2017 ◽  
Author(s):  
Anthony K. Kang ◽  
Vincent A Bielinski ◽  
Tayah M. Bolt ◽  
Christopher L Dupont ◽  
Philip D Weyman
Keyword(s):  
Phaeodactylum Tricornutum ◽  
Secretion System ◽  
Type Iv Secretion ◽  
Dna Transfer ◽  
Type Iv Secretion System ◽  
Chromosomal Integration ◽  
E Coli ◽  
Type Iv ◽  
Conjugative Gene Transfer ◽  
Conjugation Process

Bacterial conjugation utilizes a type IV secretion system and a DNA transfer mechanism to deliver DNA from one cell to another. Conjugative partners are conventionally confined to the prokaryotic domain. In a prominent exception, Agrobacterium tumefaciens type IV secretion-mediated transfer of DNA to plant cells can result in subsequent chromosomal integration. Recently, we demonstrated interdomain conjugation from Escherichia coli to the diatom Phaeodactylum tricornutum with the subsequent maintenance of an episome at chromosomal copy numbers if it contains diatom centromeres or centromere-like elements. The genes involved in the conjugation process can be separated into those encoding the type IV secretion system, also called the mating pair formation (MPF) genes, and genes involved in DNA processing called the mobilization (MOB) genes. Various protein families compose each class of conjugation genes, including common MOB types F, P, and Q and MPF types F, P, and T. The conjugative transfer from E. coli to P. tricornutum was demonstrated with a vector expressing MOBP and MTFP. Here we show that the MOBPsystem can be deleted and complemented with a MOBQ system in E.coli-diatom conjugations with subsequent episomal maintenaince. Utilization of both MOBP and MOBQ systems results in substantially higher efficiencies in E. coli-diatom conjugation. Finally, we demonstrate conjugative gene transfer between P. tricornutum and A. tumefaciens expressing a MPFT, the first demonstration of this system in diatoms,resulting in episomal maintainance or chromosomal integration, depending on the ex-conjugant. The promiscuity of MOB and MTF systems permitting prokaryote to diatom conjugative DNA transfer suggest major environmental and evolutionary importance of this process. The increased efficiency of dual MOB systems immediately improves genetic engineering in diatoms and has interesting basic cellular biology implications.

scholarly journals Host manipulation by bacterial type III and type IV secretion system effector proteases

2021 ◽  
Author(s):  
Flávia Viana ◽  
Shruthi Sachidanandan Peringathara ◽  
Arshad Rizvi ◽  
Gunnar N. Schroeder
Keyword(s):  
Secretion System ◽  
Type Iv Secretion ◽  
Type Iv Secretion System ◽  
Host Manipulation ◽  
Type Iii ◽  
Type Iv ◽  
Bacterial Type

scholarly journals In vivostructures of theHelicobacter pylori cagtype IV secretion system

2017 ◽  
Author(s):  
Yi-Wei Chang ◽  
Carrie L. Shaffer ◽  
Lee A. Rettberg ◽  
Debnath Ghosal ◽  
Grant J. Jensen
Keyword(s):  
Secretion System ◽  
Type Iv Secretion ◽  
Host Cells ◽  
Type Iv Secretion System ◽  
Molecular Architecture ◽  
Type Iv ◽  
H Pylori ◽  
Electron Cryotomography ◽  
Rod Structures ◽  
Bacterial Type

SummaryThe bacterial type IV secretion system (T4SS) is a versatile nanomachine that translocates diverse effector molecules between microbes and into eukaryotic cells. Using electron cryotomography, here we reveal the molecular architecture of the cancer-associatedHelicobacter pylori cagT4SS. Although most components are unique toH. pylori, thecagT4SS exhibits remarkable architectural similarity to previously studied T4SSs. WhenH. pyloriencounters host cells, however, the bacterium elaborates rigid, membranous tubes perforated by lateral ports. Dense, pilus-like rod structures extending from the inner membrane were also observed. We propose that the membrane tubes assemble out of the T4SS and are the delivery system forcagT4SS cargo. These studies reveal the architecture of a dynamic molecular machine that evolved to function in the human gastric niche.

scholarly journals Additional conjugation systems for inter-domain plasmid transfer to the diatom Phaeodactylum tricornutum

2017 ◽  
Author(s):  
Anthony K. Kang ◽  
Vincent A Bielinski ◽  
Tayah M. Bolt ◽  
Christopher L Dupont ◽  
Philip D Weyman
Keyword(s):  
Phaeodactylum Tricornutum ◽  
Secretion System ◽  
Type Iv Secretion ◽  
Dna Transfer ◽  
Type Iv Secretion System ◽  
Chromosomal Integration ◽  
E Coli ◽  
Type Iv ◽  
Conjugative Gene Transfer ◽  
Conjugation Process

Bacterial conjugation utilizes a type IV secretion system and a DNA transfer mechanism to deliver DNA from one cell to another. Conjugative partners are conventionally confined to the prokaryotic domain. In a prominent exception, Agrobacterium tumefaciens type IV secretion-mediated transfer of DNA to plant cells can result in subsequent chromosomal integration. Recently, we demonstrated interdomain conjugation from Escherichia coli to the diatom Phaeodactylum tricornutum with the subsequent maintenance of an episome at chromosomal copy numbers if it contains diatom centromeres or centromere-like elements. The genes involved in the conjugation process can be separated into those encoding the type IV secretion system, also called the mating pair formation (MPF) genes, and genes involved in DNA processing called the mobilization (MOB) genes. Various protein families compose each class of conjugation genes, including common MOB types F, P, and Q and MPF types F, P, and T. The conjugative transfer from E. coli to P. tricornutum was demonstrated with a vector expressing MOBP and MTFP. Here we show that the MOBPsystem can be deleted and complemented with a MOBQ system in E.coli-diatom conjugations with subsequent episomal maintenaince. Utilization of both MOBP and MOBQ systems results in substantially higher efficiencies in E. coli-diatom conjugation. Finally, we demonstrate conjugative gene transfer between P. tricornutum and A. tumefaciens expressing a MPFT, the first demonstration of this system in diatoms,resulting in episomal maintainance or chromosomal integration, depending on the ex-conjugant. The promiscuity of MOB and MTF systems permitting prokaryote to diatom conjugative DNA transfer suggest major environmental and evolutionary importance of this process. The increased efficiency of dual MOB systems immediately improves genetic engineering in diatoms and has interesting basic cellular biology implications.

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