scholarly journals Pooling Strategies to Improve Throughput of High Resolution Mass Spectrometry‐Based Analysis of Xenobiotic Metabolites Generated from Huh7 Human Hepatoma Cell Lines Transduced with P450 Enzymes

2021 ◽  
Vol 35 (S1) ◽  
Author(s):  
Choon‐myung Lee ◽  
Ken Liu ◽  
Grant Singer ◽  
Gary Miller ◽  
Shuzhao Li ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Resolution ◽  
Cell Lines ◽  
High Resolution Mass Spectrometry ◽  
Hepatoma Cell ◽  
Human Hepatoma ◽  
Human Hepatoma Cell ◽  
Hepatoma Cell Lines ◽  
Pooling Strategies ◽  
Resolution Mass

scholarly journals The Limonoids TS3 and Rubescin E Induce Apoptosis in Human Hepatoma Cell Lines and Interfere with NF-κB Signaling

PLoS ONE ◽  
2016 ◽  
Vol 11 (8) ◽  
pp. e0160843 ◽  
Author(s):  
Nicole Lange ◽  
Armelle Tsamo Tontsa ◽  
Claudia Wegscheid ◽  
Pierre Mkounga ◽  
Augustin Ephrem Nkengfack ◽  
...  
Keyword(s):  
Cell Lines ◽  
Hepatoma Cell ◽  
Human Hepatoma ◽  
Human Hepatoma Cell ◽  
Hepatoma Cell Lines

Significant decrease in telomerase activity during differentiation of human hepatoma cell lines

1998 ◽  
Vol 114 ◽  
pp. A1309 ◽  
Author(s):  
M. Nakamura ◽  
H. Saito ◽  
H. Ebinuma ◽  
M. Takahashi ◽  
F. Kaneko ◽  
...  
Keyword(s):  
Cell Lines ◽  
Hepatoma Cell ◽  
Telomerase Activity ◽  
Human Hepatoma ◽  
Human Hepatoma Cell ◽  
Hepatoma Cell Lines

Expression of HIP/PAP mRNA in Human Hepatoma Cell Lines

2006 ◽  
Vol 963 (1) ◽  
pp. 53-58 ◽  
Author(s):  
M. CERVELLO ◽  
L. GIANNITRAPANI ◽  
M. ROSA ◽  
M. NOTARBARTOLO ◽  
N. D'ALESSANDRO ◽  
...  
Keyword(s):  
Cell Lines ◽  
Hepatoma Cell ◽  
Human Hepatoma ◽  
Human Hepatoma Cell ◽  
Hepatoma Cell Lines

Chromosomes of human hepatoma cell lines

1982 ◽  
Vol 30 (1) ◽  
pp. 27-33 ◽  
Author(s):  
Daniela Simon ◽  
David P. Aden ◽  
Barbara B. Knowles
Keyword(s):  
Cell Lines ◽  
Hepatoma Cell ◽  
Human Hepatoma ◽  
Human Hepatoma Cell ◽  
Hepatoma Cell Lines

scholarly journals Modulation of c-mycExpression by Transforming Growth Factor β1 in Human Hepatoma Cell Lines

1990 ◽  
Vol 81 (3) ◽  
pp. 216-219 ◽  
Author(s):  
Nobuyuki Ito ◽  
Sumio Kawata ◽  
Shinji Tamura ◽  
Kenji Takaishi ◽  
Ryuzo Saitoh ◽  
...  
Keyword(s):  
Growth Factor ◽  
Cell Lines ◽  
Transforming Growth Factor ◽  
Hepatoma Cell ◽  
Transforming Growth Factor Β1 ◽  
Human Hepatoma ◽  
Human Hepatoma Cell ◽  
Hepatoma Cell Lines

scholarly journals Suppression of DNA synthesis by HMG-CoA reductase inhibitor in human hepatoma cell lines.

Kanzo ◽  
1989 ◽  
Vol 30 (5) ◽  
pp. 598-599
Author(s):  
Ryuzo SAITOH ◽  
Sumio KAWATA ◽  
Shinji TAMURA ◽  
Nobuyuki ITO ◽  
Kenji TAKAISHI ◽  
...  
Keyword(s):  
Dna Synthesis ◽  
Cell Lines ◽  
Reductase Inhibitor ◽  
Hepatoma Cell ◽  
Human Hepatoma ◽  
Human Hepatoma Cell ◽  
Hmg Coa Reductase ◽  
Hepatoma Cell Lines ◽  
Hmg Coa Reductase Inhibitor ◽  
Coa Reductase

scholarly journals Effect of 5-fluorouracil on mRNA expression of drug metabolizing enzyme and transporter genes in human hepatoma cell lines

2021 ◽  
Vol 42 (4) ◽  
pp. 121-127
Author(s):  
Hideo SHIOHIRA ◽  
Koya FUKUNAGA ◽  
Jose Carlos S. TAYAG ◽  
Yurika TAMASHIRO ◽  
Taisei MUSHIRODA ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Cell Lines ◽  
Hepatoma Cell ◽  
Human Hepatoma ◽  
Human Hepatoma Cell ◽  
Hepatoma Cell Lines ◽  
Drug Metabolizing Enzyme ◽  
Metabolizing Enzyme

scholarly journals Biosynthesis of factor XIII B subunit by human hepatoma cell lines

Blood ◽  
1986 ◽  
Vol 68 (6) ◽  
pp. 1272-1279 ◽  
Author(s):  
JA Nagy ◽  
P Henriksson ◽  
J McDonagh
Keyword(s):  
Conditioned Medium ◽  
Cell Lines ◽  
Hepatoma Cell ◽  
Human Hepatoma ◽  
Hep G2 ◽  
Human Hepatoma Cell ◽  
Hepatoma Cell Lines ◽  
B Subunit ◽  
Cell Lysate ◽  
Crossed Immunoelectrophoresis

Abstract The plasma transglutaminase, factor XIIIa (FXIIIa), circulates as a zymogen containing two proteins, A and B, arranged in a noncovalent tetrameric complex, A2B2. Biosynthesis of plasma FXIII has not previously been demonstrated. In the present study, direct evidence has been obtained that two human hepatoma cell lines, Hep G2 and PLC/PRF/5, synthesize and secrete FXIII B protein. Secretion of the B subunit of FXIII by Hep G2 was demonstrated by immunoblotting. De novo synthesis by Hep G2 was confirmed in 35S-methionine-labeled cultures. Radiolabeled conditioned medium was concentrated, mixed (1:1) with purified B protein, and examined by crossed immunoelectrophoresis with antiserum to the B subunit. The single protein precipitin arc of purified B protein comigrated with the radiolabeled FXIII from Hep G2 visualized by autoradiography, indicating both electrophoretic and antigenic identity. The data presented here represent the first demonstrations of biosynthesis of FXIII B protein by any cell type and suggest that the liver is the site of synthesis of FXIII B protein. Further analysis of concentrated Hep G2 serum-free conditioned medium (SFCM) and cell lysate by immunoblotting following nondenaturing agarose gel electrophoresis demonstrated the FXIII A protein as well as the B protein and also revealed synthesis and secretion of the A and B proteins by PLC/PRF/5. Crossed immunoelectrophoresis studies of Hep G2 SFCM and cell lysate suggest that Hep G2 cells also synthesize and secrete the plasma FXIII zymogen. With a specific radioimmunoassay for B protein, FXIII was found in Hep G2 SFCM at approximately 4 ng/mL; with an amplified rocket immunoelectrophoresis technique the level was approximately 5 ng/mL.

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