scholarly journals A multilayer design of parallel‐plate flow chamber for studies of endothelial cell response to fluid shear stress

2007 ◽  
Vol 21 (5) ◽  
Author(s):  
Sungkwon Kang ◽  
Won Hee Lee ◽  
Anjali Hirani ◽  
Pavlos P Vlachos ◽  
Yong Woo Lee
Keyword(s):  
Shear Stress ◽  
Endothelial Cell ◽  
Cell Response ◽  
Parallel Plate ◽  
Fluid Shear Stress ◽  
Flow Chamber ◽  
Fluid Shear ◽  
Parallel Plate Flow Chamber ◽  
Multilayer Design ◽  
Endothelial Cell Response

A mechanosensory complex that mediates the endothelial cell response to fluid shear stress

Nature ◽  
2005 ◽  
Vol 437 (7057) ◽  
pp. 426-431 ◽  
Author(s):  
Eleni Tzima ◽  
Mohamed Irani-Tehrani ◽  
William B. Kiosses ◽  
Elizabetta Dejana ◽  
David A. Schultz ◽  
...  
Keyword(s):  
Shear Stress ◽  
Endothelial Cell ◽  
Cell Response ◽  
Fluid Shear Stress ◽  
Fluid Shear ◽  
Endothelial Cell Response

T-786C Polymorphism of thenos-3Gene and the Endothelial Cell Response to Fluid Shear Stress—A Proteome Analysis

2009 ◽  
Vol 8 (6) ◽  
pp. 3161-3168 ◽  
Author(s):  
Abdul R. Asif ◽  
Michael Oellerich ◽  
Victor William Armstrong ◽  
Markus Hecker ◽  
Marco Cattaruzza
Keyword(s):  
Shear Stress ◽  
Endothelial Cell ◽  
Cell Response ◽  
Fluid Shear Stress ◽  
Proteome Analysis ◽  
Fluid Shear ◽  
Endothelial Cell Response

scholarly journals The roles of focal adhesion and cytoskeleton systems in fluid shear stress-induced endothelial cell response

BIOCELL ◽  
2020 ◽  
Vol 44 (2) ◽  
pp. 137-145
Author(s):  
KHAWAR ALI SHAHZAD ◽  
ZHONGJIE QIN ◽  
YAN LI ◽  
DELIN XIA
Keyword(s):  
Shear Stress ◽  
Endothelial Cell ◽  
Focal Adhesion ◽  
Cell Response ◽  
Fluid Shear Stress ◽  
Fluid Shear ◽  
Endothelial Cell Response

Fluid shear stress increases membrane fluidity in endothelial cells: a study with DCVJ fluorescence

2000 ◽  
Vol 278 (4) ◽  
pp. H1401-H1406 ◽  
Author(s):  
Mark A. Haidekker ◽  
Nicolas L'Heureux ◽  
John A. Frangos
Keyword(s):  
Endothelial Cells ◽  
Shear Stress ◽  
Membrane Fluidity ◽  
Fluid Shear Stress ◽  
Cell Metabolism ◽  
Cell Monolayer ◽  
Flow Chamber ◽  
Fluid Shear ◽  
Parallel Plate Flow Chamber ◽  
Entire Cell

Fluid shear stress (FSS) has been shown to be an ubiquitous stimulator of mammalian cell metabolism. Although many of the intracellular signal transduction pathways have been characterized, the primary mechanoreceptor for FSS remains unknown. One hypothesis is that the cytoplasmic membrane acts as the receptor for FSS, leading to increased membrane fluidity, which in turn leads to the activation of heterotrimetric G proteins (13). 9-(Dicyanovinyl)-julolidine (DCVJ) is a fluorescent probe that integrates into the cell membrane and changes its quantum yield with the viscosity of the environment. In a parallel-plate flow chamber, confluent layers of DCVJ-labeled human endothelial cells were exposed to different levels of FSS. With increased FSS, a reduced fluorescence intensity was observed, indicating an increase of membrane fluidity. Step changes of FSS caused an approximately linear drop of fluorescence within 5 s, showing fast and almost full recovery after shear cessation. A linear dose-response relationship between shear stress and membrane fluidity changes was observed. The average fluidity increase over the entire cell monolayer was 22% at 26 dyn/cm2. This study provides evidence for a link between FSS and membrane fluidity, and suggests that the membrane is an important flow mechanosensor of the cell.

Three Distinct Types of Morphological Responses of Cultured Vascular Endothelial Cells to Physiological Levels of Fluid Shear Stress

2003 ◽  
Author(s):  
Michiaka Masuda ◽  
Keigi Fujiwara
Keyword(s):  
Endothelial Cells ◽  
Shear Stress ◽  
Random Walk ◽  
Endothelial Cell ◽  
Parallel Plate ◽  
Fluid Shear Stress ◽  
Vascular Endothelial Cells ◽  
Vascular Endothelial ◽  
Fluid Shear ◽  
Flow Apparatus

Vascular endothelial cells are known to respond to fluid shear stress. To gain insights into the mechanism of flow response by these cells, various types of in vitro devices in which endothelial cells can be cultured under flowing culture medium have been designed. Using such a device, one can apply known levels of (usually laminar) fluid shear stress to cultured endothelial cells. We have made two types of devices: a viscometer-based cone-and-plate flow apparatus and a parallel plate chamber. The cone-and-plate apparatus is used to do biochemical analyses of flow effects on cells while the parallel plate chamber is used to observe dynamic behavior of endothelial cells under flow. We were able to maintain confluent endothelial cell cultures under flow for over a week in the parallel plate flow apparatus. Using this chamber and high resolution time-lapse video microscopy, we studied morphological changes of endothelial cells exposed to different levels of fluid shear stress. We found that endothelial cells in a confluent monolayer exhibited three types of fluid shear stress level-dependent morphological and motile responses within a narrow fluid shear stress range between 0.1–10 dyn/cm2. Endothelial cells cultured under no flow exhibited variable shapes and no preferred orientation of their long cell axes and showed a jiggling motion. When exposed to fluid shear stress levels of below 0.5 dyn/cm2, endothelial cell morphology and motility were not affected. However, when fluid shear stress levels were increased to 2–4 dyn/cm2, they became polygonal and showed increased random-walk activity. Fluid shear stress over 6 dyn/cm2 caused endothelial cells to initially become polygonal and increase their random-walk activity, but they soon became elongated and aligned in the direction of flow. As the cells elongated and aligned, they migrated in the direction of flow. The average velocity of this directed cell migration was less than that of cells moving randomly under the same flow condition at earlier times. These observations indicate that endothelial cells are able to detect and respond to a surprisingly small change in fluid shear stress. It is possible that endothelial cell physiology in vivo is also regulated by small changes in fluid shear stress and that a fluid shear stress change of a few dynes per cm2 within a certain region of an artery could trigger atherogenesis in that particular location.

scholarly journals Turbulent fluid shear stress induces vascular endothelial cell turnover in vitro.

1986 ◽  
Vol 83 (7) ◽  
pp. 2114-2117 ◽  
Author(s):  
P. F. Davies ◽  
A. Remuzzi ◽  
E. J. Gordon ◽  
C. F. Dewey ◽  
M. A. Gimbrone
Keyword(s):  
Shear Stress ◽  
Endothelial Cell ◽  
Fluid Shear Stress ◽  
Vascular Endothelial Cell ◽  
Vascular Endothelial ◽  
Cell Turnover ◽  
Fluid Shear ◽  
Turbulent Fluid
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