scholarly journals Definition of the DRB1*0901 peptide binding motif within novel DRB1*0901 restricted T cell epitopes by peptide binding and structural modeling

2008 ◽  
Vol 22 (S1) ◽  
Author(s):  
Eddie A James ◽  
Antonis K Moustakas ◽  
George K Papadopoulos ◽  
Randi S Nouv ◽  
William W Kwok
Keyword(s):  
T Cell ◽  
Peptide Binding ◽  
Structural Modeling ◽  
Binding Motif ◽  
T Cell Epitopes ◽  
Peptide Binding Motif ◽  
Definition Of

scholarly journals Definition of the peptide binding motif within DRB1*1401 restricted epitopes by peptide competition and structural modeling

2008 ◽  
Vol 45 (9) ◽  
pp. 2651-2659 ◽  
Author(s):  
Eddie A. James ◽  
Antonis K. Moustakas ◽  
DeAnna Berger ◽  
Laurie Huston ◽  
George K. Papadopoulos ◽  
...  
Keyword(s):  
Peptide Binding ◽  
Structural Modeling ◽  
Binding Motif ◽  
Peptide Binding Motif ◽  
Definition Of

Definition of an extended MHC class II-peptide binding motif for the autoimmune disease-associated Lewis rat RT1.BL molecule

1997 ◽  
Vol 56 ◽  
pp. 239
Author(s):  
M.C. Grosfeld-Stulemeyer ◽  
I. Joosten ◽  
M. van der Kraan ◽  
M.H.M. Wauben
Keyword(s):  
Autoimmune Disease ◽  
Mhc Class Ii ◽  
Peptide Binding ◽  
Class Ii ◽  
Binding Motif ◽  
Lewis Rat ◽  
Peptide Binding Motif ◽  
Definition Of

scholarly journals Definition of naturally processed peptides reveals convergent presentation of autoantigenic topoisomerase-I epitopes in scleroderma

2019 ◽  
Author(s):  
Eleni Tiniakou ◽  
Andrea Fava ◽  
Zsuzsanna H. McMahan ◽  
Tara Guhr ◽  
Robert N. O’Meally ◽  
...  
Keyword(s):  
T Cells ◽  
T Cell ◽  
Lung Disease ◽  
Topoisomerase I ◽  
Antigen Processing ◽  
Peptide Binding ◽  
Cd4 T Cell ◽  
Binding Motif ◽  
T Cell Epitopes ◽  
Hla Dr

AbstractDisease-associated HLA-DRB1 alleles are thought to confer risk of developing autoimmunity by favoring the presentation of select autoantigenic epitopes. However, identification of these epitopes and the principles governing their presentation has been hindered by the imprecision of currently available methods, which cannot fully recapitulate the complexity of human pathophysiology. We present a natural antigen processing assay (NAPA), which overcomes these limitations by studying the presentation of autoantigenic CD4+ T cell epitopes by monocyte-derived dendritic cells (mo-DCs) from patients. We applied this strategy to study the processing and presentation of topoisomerase-1 (TOP1), a prevalent autoantigen in scleroderma that is associated with lung fibrosis and high mortality. We found that a common set of 10 epitopes was presented by mo-DCs from patients with diverse HLA-DR variants, including those not previously associated with the disease. Sequence analysis revealed a shared peptide-binding motif within the HLA-DR peptide binding grooves of patients who developed anti-TOP1 autoantibodies. In addition, a subset of naturally presented TOP1 peptides were characterized by immunological promiscuity, as they could bind to diverse HLA-DR peptide binding grooves. NAPA epitopes were immunorelevant: they could stimulate autoreactive CD4+ T cells in patients, and the number of epitopes recognized correlated with lung disease severity. These findings mechanistically implicate presentation of a convergent set of TOP1 epitopes in the development of scleroderma lung disease. Precise identification of autoantigenic epitopes is key to understanding the primordial mechanisms for the loss of tolerance, studying disease-propagating autoreactive T cells, and developing antigen-specific immunotherapy.One Sentence SummaryUse of a novel natural antigen processing assay reveals a mechanism for the presentation of shared CD4+ T cell epitopes of topoisomerase-I in immunogenetically diverse patients with scleroderma.

scholarly journals Characterization of the Canine MHC Class I DLA-88*50101 Peptide Binding Motif as a Prerequisite for Canine T Cell Immunotherapy

PLoS ONE ◽  
2016 ◽  
Vol 11 (11) ◽  
pp. e0167017 ◽  
Author(s):  
Sharon M. Barth ◽  
Christian M. Schreitmüller ◽  
Franziska Proehl ◽  
Kathrin Oehl ◽  
Leonie M. Lumpp ◽  
...  
Keyword(s):  
T Cell ◽  
Mhc Class I ◽  
Peptide Binding ◽  
Class I ◽  
Binding Motif ◽  
Cell Immunotherapy ◽  
Peptide Binding Motif ◽  
T Cell Immunotherapy

scholarly journals A Peptide-binding Motif for I-Ag7, the Class II Major Histocompatibility Complex (MHC) Molecule of NOD and Biozzi AB/H Mice

1997 ◽  
Vol 185 (6) ◽  
pp. 1013-1022 ◽  
Author(s):  
Leonard C. Harrison ◽  
Margo C. Honeyman ◽  
Sylvie Trembleau ◽  
Silvia Gregori ◽  
Fabio Gallazzi ◽  
...  
Keyword(s):  
Major Histocompatibility Complex ◽  
T Cell ◽  
Critical Role ◽  
Peptide Binding ◽  
Class Ii ◽  
Binding Motif ◽  
T Cell Epitopes ◽  
Major Histocompatibility ◽  
Histocompatibility Complex ◽  
Hla Dq

The class II major histocompatibility complex molecule I-Ag7 is strongly linked to the development of spontaneous insulin-dependent diabetes mellitus (IDDM) in non obese diabetic mice and to the induction of experimental allergic encephalomyelitis in Biozzi AB/H mice. Structurally, it resembles the HLA-DQ molecules associated with human IDDM, in having a non-Asp residue at position 57 in its β chain. To identify the requirements for peptide binding to I-Ag7 and thereby potentially pathogenic T cell epitopes, we analyzed a known I-Ag7-restricted T cell epitope, hen egg white lysozyme (HEL) amino acids 9–27. NH2- and COOH-terminal truncations demonstrated that the minimal epitope for activation of the T cell hybridoma 2D12.1 was M12-R21 and the minimum sequence for direct binding to purified I-Ag7 M12-Y20/ K13-R21. Alanine (A) scanning revealed two primary anchors for binding at relative positions (p) 6 (L) and 9 (Y) in the HEL epitope. The critical role of both anchors was demonstrated by incorporating L and Y in poly(A) backbones at the same relative positions as in the HEL epitope. Well-tolerated, weakly tolerated, and nontolerated residues were identified by analyzing the binding of peptides containing multiple substitutions at individual positions. Optimally, p6 was a large, hydrophobic residue (L, I, V, M), whereas p9 was aromatic and hydrophobic (Y or F) or positively charged (K, R). Specific residues were not tolerated at these and some other positions. A motif for binding to I-Ag7 deduced from analysis of the model HEL epitope was present in 27/30 (90%) of peptides reported to be I-Ag7–restricted T cell epitopes or eluted from I-Ag7. Scanning a set of overlapping peptides encompassing human proinsulin revealed the motif in 6/6 good binders (sensitivity = 100%) and 4/13 weak or non-binders (specificity = 70%). This motif should facilitate identification of autoantigenic epitopes relevant to the pathogenesis and immunotherapy of IDDM.

Selection of Self-reactive Peptides Within Human Aggrecan by use of a HLA-DRB1*0401 Peptide Binding Motif

1997 ◽  
Vol 10 (6) ◽  
pp. 569-578 ◽  
Author(s):  
A.Mieke H Boots ◽  
Gijs F.M Verheijden ◽  
Ron Schöningh ◽  
Catherina J van Staveren ◽  
Ebo Bos ◽  
...  
Keyword(s):  
Peptide Binding ◽  
Binding Motif ◽  
Peptide Binding Motif ◽  
Selection Of

Synthetic peptide libraries in the determination of T cell epitopes and peptide binding specificity of class I molecules

1992 ◽  
Vol 22 (6) ◽  
pp. 1405-1412 ◽  
Author(s):  
Ton N. M. Schumacher ◽  
Grada M. Van Bleek ◽  
Marie-ThéRèSe Heemels ◽  
Karl Deres ◽  
Ka Wan Li ◽  
...  
Keyword(s):  
T Cell ◽  
Synthetic Peptide ◽  
Peptide Binding ◽  
Binding Specificity ◽  
Peptide Libraries ◽  
Class I ◽  
T Cell Epitopes ◽  
Peptide Binding Specificity

The peptide binding motif of the disease associated HLA-DQ (α 1* 0501, β 1* 0201) molecule

1996 ◽  
Vol 26 (11) ◽  
pp. 2764-2772 ◽  
Author(s):  
Frode Vartdal ◽  
Bente H. Johansen ◽  
Thomas Friede ◽  
Christopher J. Thorpe ◽  
Stefan Stevanović ◽  
...  
Keyword(s):  
Peptide Binding ◽  
Binding Motif ◽  
Hla Dq ◽  
Peptide Binding Motif
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