scholarly journals Differential Response of Salivary Gland Cell Types to Gamma‐tocopherol Treatments

2011 ◽  
Vol 25 (S1) ◽  
Author(s):  
Leonard E. Gerber ◽  
Cynthia L. Jackson ◽  
Chung J Cha ◽  
Douglas R. Gnepp
Keyword(s):  
Salivary Gland ◽  
Gland Cell ◽  
Cell Types ◽  
Differential Response ◽  
Salivary Gland Cell ◽  
Gamma Tocopherol

Raman hyperspectral imaging of different salivary gland cell types for tissue engineering

2019 ◽  
Author(s):  
Ting Chean Khoo ◽  
Nicholas Moskwa ◽  
Anna Sharikova ◽  
Melinda Larsen ◽  
Alexander T. Khmaladze
Keyword(s):  
Tissue Engineering ◽  
Salivary Gland ◽  
Hyperspectral Imaging ◽  
Gland Cell ◽  
Cell Types ◽  
Salivary Gland Cell

scholarly journals Alginate Hydrogel Microtubes for Salivary Gland Cell Organization and Cavitation

2022 ◽  
Vol 9 (1) ◽  
pp. 38
Author(s):  
Matthew Jorgensen ◽  
Pujhitha Ramesh ◽  
Miriam Toro ◽  
Emily Evans ◽  
Nicholas Moskwa ◽  
...  
Keyword(s):  
Salivary Gland ◽  
Gland Cell ◽  
Mesenchymal Cell ◽  
Cell Types ◽  
Salivary Gland Cell ◽  
Cellular Organization ◽  
Alginate Hydrogel ◽  
Practical Applications ◽  
Model Cell ◽  
Cell Organization

Understanding the different regulatory functions of epithelial and mesenchymal cell types in salivary gland development and cellular organization is essential for proper organoid formation and salivary gland tissue regeneration. Here, we demonstrate a biocompatible platform using pre-formed alginate hydrogel microtubes to facilitate direct epithelial–mesenchymal cell interaction for 3D salivary gland cell organization, which allows for monitoring cellular organization while providing a protective barrier from cell-cluster loss during medium changes. Using mouse salivary gland ductal epithelial SIMS cells as the epithelial model cell type and NIH 3T3 fibroblasts or primary E16 salivary mesenchyme cells as the stromal model cell types, self-organization from epithelial–mesenchymal interaction was examined. We observed that epithelial and mesenchymal cells undergo aggregation on day 1, cavitation by day 4, and generation of an EpCAM-expressing epithelial cell layer as early as day 7 of the co-culture in hydrogel microtubes, demonstrating the utility of hydrogel microtubes to facilitate heterotypic cell–cell interactions to form cavitated organoids. Thus, pre-formed alginate microtubes are a promising co-culture method for further understanding epithelial and mesenchymal interaction during tissue morphogenesis and for future practical applications in regenerative medicine.

Matrigel improves functional properties of human submandibular salivary gland cell line

2011 ◽  
Vol 43 (4) ◽  
pp. 622-631 ◽  
Author(s):  
Ola M. Maria ◽  
Osama Maria ◽  
Younan Liu ◽  
Svetlana V. Komarova ◽  
Simon D. Tran
Keyword(s):  
Salivary Gland ◽  
Cell Line ◽  
Functional Properties ◽  
Gland Cell ◽  
Salivary Gland Cell ◽  
Submandibular Salivary Gland

scholarly journals Salivary gland cell aggregates are derived from self-organization of acinar lineage cells

2019 ◽  
Vol 97 ◽  
pp. 122-130 ◽  
Author(s):  
Jomy J. Varghese ◽  
M. Eva Hansen ◽  
Azmeer Sharipol ◽  
Matthew H. Ingalls ◽  
Martha A. Ormanoski ◽  
...  
Keyword(s):  
Salivary Gland ◽  
Gland Cell ◽  
Self Organization ◽  
Salivary Gland Cell ◽  
Cell Aggregates

scholarly journals Functional analysis of the biochemical activity of mammalian phosphatidylinositol 5 phosphate 4-kinase enzymes

2019 ◽  
Vol 39 (2) ◽  
Author(s):  
Swarna Mathre ◽  
K. Balasankara Reddy ◽  
Visvanathan Ramya ◽  
Harini Krishnan ◽  
Avishek Ghosh ◽  
...  
Keyword(s):  
Salivary Gland ◽  
Cell Size ◽  
Kinase Activity ◽  
Gland Cell ◽  
Specific Activity ◽  
Salivary Gland Cell ◽  
Drosophila Genome ◽  
Biochemical Activity

Abstract Phosphatidylinositol 5 phosphate 4-kinase (PIP4K) are enzymes that catalyse the phosphorylation of phosphatidylinositol 5-phosphate (PI5P) to generate PI(4,5)P2. Mammalian genomes contain three genes, PIP4K2Α, 2B and 2C and murine knockouts for these suggested important physiological roles in vivo. The proteins encoded by PIP4K2A, 2B and 2C show widely varying specific activities in vitro; PIP4K2A is highly active and PIP4K2C 2000-times less active, and the relationship between this biochemical activity and in vivo function is unknown. By contrast, the Drosophila genome encodes a single PIP4K (dPIP4K) that shows high specific activity in vitro and loss of this enzyme results in reduced salivary gland cell size in vivo. We find that the kinase activity of dPIP4K is essential for normal salivary gland cell size in vivo. Despite their highly divergent specific activity, we find that all three mammalian PIP4K isoforms are able to enhance salivary gland cell size in the Drosophila PIP4K null mutant implying a lack of correlation between in vitro activity measurements and in vivo function. Further, the kinase activity of PIP4K2C, reported to be almost inactive in vitro, is required for in vivo function. Our findings suggest the existence of unidentified factors that regulate PIP4K enzyme activity in vivo.

Interferon-γ induced cell death in a cultured human salivary gland cell line

1996 ◽  
Vol 167 (2) ◽  
pp. 297-304 ◽  
Author(s):  
Ava J. Wu ◽  
Zhi Jian Chen ◽  
Maria Tsokos ◽  
Brian C. O'Connell ◽  
Indu S. Ambudkar ◽  
...  
Keyword(s):  
Cell Death ◽  
Salivary Gland ◽  
Cell Line ◽  
Gland Cell ◽  
Interferon Γ ◽  
Salivary Gland Cell ◽  
Human Salivary Gland

Effect of tumor necrosis factor-? and interferon-? on the growth of a human salivary gland cell line

1994 ◽  
Vol 161 (2) ◽  
pp. 217-226 ◽  
Author(s):  
Ava J. Wu ◽  
Regina H. Kurrasch ◽  
Joseph Katz ◽  
Philip C. Fox ◽  
Bruce J. Baum ◽  
...  
Keyword(s):  
Salivary Gland ◽  
Tumor Necrosis Factor ◽  
Cell Line ◽  
Tumor Necrosis ◽  
Gland Cell ◽  
Salivary Gland Cell ◽  
Human Salivary Gland ◽  
Necrosis Factor

scholarly journals A Steroid-Triggered Transcriptional Hierarchy Controls Salivary Gland Cell Death during Drosophila Metamorphosis

2000 ◽  
Vol 5 (3) ◽  
pp. 445-455 ◽  
Author(s):  
Changan Jiang ◽  
Anne-Françoise J. Lamblin ◽  
Hermann Steller ◽  
Carl S. Thummel
Keyword(s):  
Cell Death ◽  
Salivary Gland ◽  
Gland Cell ◽  
Salivary Gland Cell
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