scholarly journals A Novel Method to Measure Glucose Uptake by Single Skeletal Muscle Fibers Reveals a Similar Level of Insulin Resistance for Type IIA, IIB, IIX and IIB/X Fibers from Obese Zucker Rat Epitrochlearis Muscle

2012 ◽  
Vol 26 (S1) ◽  
Author(s):  
James G. MacKrell ◽  
Gregory D. Cartee
Keyword(s):  
Insulin Resistance ◽  
Skeletal Muscle ◽  
Glucose Uptake ◽  
Muscle Fibers ◽  
Zucker Rat ◽  
Skeletal Muscle Fibers ◽  
Obese Zucker Rat ◽  
Novel Method

scholarly journals High-Fat Diet-Induced Insulin Resistance in Single Skeletal Muscle Fibers is Fiber Type Selective

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Mark W. Pataky ◽  
Haiyan Wang ◽  
Carmen S. Yu ◽  
Edward B. Arias ◽  
Robert J. Ploutz-Snyder ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
Skeletal Muscle ◽  
High Fat Diet ◽  
Fiber Type ◽  
Muscle Fibers ◽  
High Fat ◽  
Skeletal Muscle Fibers

scholarly journals Fibroblast growth factor-21 potentiates glucose transport in skeletal muscle fibers

2020 ◽  
Vol 65 (3) ◽  
pp. 85-95
Author(s):  
G Rosales-Soto ◽  
A Diaz-Vegas ◽  
M Casas ◽  
A Contreras-Ferrat ◽  
E Jaimovich
Keyword(s):  
Skeletal Muscle ◽  
Growth Factor ◽  
Glucose Uptake ◽  
Fibroblast Growth Factor ◽  
Glucose Transport ◽  
Muscle Fibers ◽  
Fibroblast Growth Factor 21 ◽  
Fibroblast Growth ◽  
Skeletal Muscle Fibers ◽  
Pkc Ζ

Fibroblast growth factor 21 (FGF21) is a pleiotropic peptide hormone that is considered a myokine playing a role in a variety of endocrine functions, including regulation of glucose transport and lipid metabolism. Although FGF21 has been associated with glucose metabolism in skeletal muscle cells, its cellular mechanism in adult skeletal muscle fibers glucose uptake is poorly understood. In the present study, we found that FGF21 induced a dose−response effect, increasing glucose uptake in skeletal muscle fibers from the flexor digitorum brevis muscle of mice, evaluated using the fluorescent glucose analog 2-NBDG (300 µM) in single living fibers. This effect was prevented by the use of either cytochalasin B (5 µM) or indinavir (100 µM), both antagonists of GLUT4 activity. The use of PI3K inhibitors such as LY294002 (50 µM) completely prevented the FGF21-dependent glucose uptake. In fibers electroporated with the construct encoding GLUT4myc-eGFP chimera and stimulated with FGF21 (100 ng/mL), a strong sarcolemmal GLUT4 label was detected. This effect promoted by FGF21 was demonstrated to be dependent on atypical PKC-ζ, by using selective PKC inhibitors. FGF21 at low concentrations potentiated the effect of insulin on glucose uptake but at high concentrations, completely inhibited the uptake in the presence of insulin. These results suggest that FGF21 regulates glucose uptake by a mechanism mediated by GLUT4 and dependent on atypical PKC-ζ in skeletal muscle.

Phosphorescence quenching method for measurement of intracellular P O 2 in isolated skeletal muscle fibers

1999 ◽  
Vol 86 (2) ◽  
pp. 720-724 ◽  
Author(s):  
Michael C. Hogan
Keyword(s):  
Skeletal Muscle ◽  
Single Cells ◽  
Muscle Fibers ◽  
Ringer Solution ◽  
Phosphorescence Lifetime ◽  
Phosphorescence Quenching ◽  
Skeletal Muscle Fibers ◽  
Lumbrical Muscle ◽  
Novel Method ◽  
Quenching Method

Values of skeletal muscle intracellular[Formula: see text] during conditions ranging from rest to maximal metabolic rates have been difficult to quantify. A method for measurement of intracellular[Formula: see text] in isolated single skeletal muscle fibers by using O2-dependent quenching of a phosphorescent-O2probe is described. Intact single skeletal muscle fibers from Xenopus laevis were dissected from the lumbrical muscle and mounted in a glass chamber containing Ringer solution at 20°C. The chamber was placed on the stage of an inverted microscope configured for epi-illumination. A solution containing palladium- meso-tetra (4-carboxyphenyl) porphine bound to bovine serum albumin was injected into single fibers by micropipette pressure injection. Phosphorescence-decay curves (average of 10 rapid flashes) were recorded every 7 s from single cells ( n = 24) in which respiration had been eliminated with NaCN, while the [Formula: see text]of the Ringer solution surrounding the cell was varied from 0 to 159 Torr. For each measurement, the phosphorescence lifetime was calculated at the varied extracellular [Formula: see text] by obtaining a best-fit estimate by using a monoexponential function. The phosphorescence lifetime varied from 40 to 70 μs at an extracellular[Formula: see text] of 159 Torr to 650–700 μs at 0 Torr. The phosphorescent lifetimes for the varied[Formula: see text] were used to calculate, by using the Stern-Volmer relationship, the phosphorescence-quenching constant (100 Torr−1 ⋅ s−1), and the phosphorescence lifetime in a zero-O2 environment (690 μs) for the phosphor within the intracellular environment. This technique demonstrates a novel method for determining intracellular[Formula: see text] in isolated single skeletal muscle fibers.

Microautoradiographic studies of glucose uptake in skeletal muscle fibers at rest

2001 ◽  
Vol 103 (4) ◽  
pp. 355-363 ◽  
Author(s):  
Hana Hayasaki ◽  
Masahisa Shimada ◽  
Kiyoto Kanbara ◽  
Masahito Watanabe
Keyword(s):  
Skeletal Muscle ◽  
Glucose Uptake ◽  
Muscle Fibers ◽  
Skeletal Muscle Fibers

scholarly journals Fibroblast Growth Factor 21 Promotes Glucose Uptake in Adult Skeletal Muscle Fibers from Mice

2018 ◽  
Vol 32 (S1) ◽  
Author(s):  
Giovanni Rosales‐Soto ◽  
Alexis Díaz‐Vegas ◽  
Paola Llanos ◽  
Enrique Jaimovich ◽  
Ariel Contreras‐Ferrat
Keyword(s):  
Skeletal Muscle ◽  
Growth Factor ◽  
Glucose Uptake ◽  
Fibroblast Growth Factor ◽  
Muscle Fibers ◽  
Fibroblast Growth Factor 21 ◽  
Fibroblast Growth ◽  
Adult Skeletal Muscle ◽  
Skeletal Muscle Fibers

The “KIMWIPE” Effect in Ultra-Thin Cryosections

1985 ◽  
Vol 43 ◽  
pp. 458-459
Author(s):  
I. Taylor ◽  
P. Ingram ◽  
J.R. Sommer
Keyword(s):  
Skeletal Muscle ◽  
Electrical Stimulation ◽  
Muscle Fibers ◽  
Ice Crystals ◽  
Crystal Formation ◽  
Ice Crystal ◽  
Thin Sections ◽  
Skeletal Muscle Fibers ◽  
Freeze Dried ◽  
Ice Crystal Formation

In studying quick-frozen single intact skeletal muscle fibers for structural and microchemical alterations that occur milliseconds, and fractions thereof, after electrical stimulation, we have developed a method to compare, directly, ice crystal formation in freeze-substituted thin sections adjacent to all, and beneath the last, freeze-dried cryosections. We have observed images in the cryosections that to our knowledge have not been published heretofore (Figs.1-4). The main features are that isolated, sometimes large regions of the sections appear hazy and have much less contrast than adjacent regions. Sometimes within the hazy regions there are smaller areas that appear crinkled and have much more contrast. We have also observed that while the hazy areas remain still, the regions of higher contrast visibly contract in the beam, often causing tears in the sections that are clearly not caused by ice crystals (Fig.3, arrows).

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