scholarly journals Myosin II is important for endothelial barrier function but is not involved in sphingosine‐1‐phosphate‐induced endothelial barrier enhancement

2013 ◽  
Vol 27 (S1) ◽  
Author(s):  
Xun Elvis Zhang ◽  
Jerome W Breslin
Keyword(s):  
Barrier Function ◽  
Myosin Ii ◽  
Endothelial Barrier ◽  
Endothelial Barrier Function ◽  
Sphingosine 1 Phosphate

scholarly journals Endothelial Barrier Function is co-regulated at Vessel Bifurcations by Fluid Forces and Sphingosine-1-Phosphate

2020 ◽  
Author(s):  
Ehsan Akbari ◽  
Griffin B. Spychalski ◽  
Miles M. Menyhert ◽  
Kaushik K. Rangharajan ◽  
Shaurya Prakash ◽  
...  
Keyword(s):  
Shear Stress ◽  
Fluid Flow ◽  
Barrier Function ◽  
Endothelial Barrier ◽  
Laminar Shear Stress ◽  
Endothelial Barrier Function ◽  
Fluid Forces ◽  
Sphingosine 1 Phosphate ◽  
Laminar Shear ◽  
Vessel Bifurcations

AbstractSphingosine-1-phosphate (S1P) is a blood-borne bioactive lipid mediator of endothelial barrier function. Prior studies have implicated mechanical stimulation due to intravascular laminar shear stress in co-regulating S1P signaling in endothelial cells (ECs). Yet, vascular networks in vivo consist of vessel bifurcations, and this geometry produces hemodynamic forces that are distinct from laminar shear stress. However, the role of these forces at vessel bifurcations in regulating S1P-dependent endothelial barrier function is not known. In this study, we implemented a microfluidic platform that recapitulates the flow dynamics of vessel bifurcations with in situ quantification of the permeability of microvessel analogues. Co-application of S1P with impinging bifurcated fluid flow, which was characterized by approximately zero shear stress and 38 dyn cm-2 stagnation pressure at the vessel bifurcation point, promotes vessel stabilization. Similarly, co-treatment of carrier-free S1P with 3 dyn cm-2 laminar shear stress is also protective of endothelial barrier function. Moreover, it is shown that vessel stabilization due to laminar shear stress, but not bifurcated fluid flow, is dependent on S1P receptor 1 or 2 signaling. Collectively, these findings demonstrate the endothelium-protective function of fluid forces at vessel bifurcations and their involvement in coordinating S1P-dependent regulation of vessel permeability.

scholarly journals Impaired endothelial barrier function in apolipoprotein M‐deficient mice is dependent on sphingosine‐1‐phosphate receptor 1

2016 ◽  
Vol 30 (6) ◽  
pp. 2351-2359 ◽  
Author(s):  
Pernille M. Christensen ◽  
Catherine H. Liu ◽  
Steven L. Swendeman ◽  
Hideru Obinata ◽  
Klaus Qvortrup ◽  
...  
Keyword(s):  
Barrier Function ◽  
Endothelial Barrier ◽  
Endothelial Barrier Function ◽  
Apolipoprotein M ◽  
Sphingosine 1 Phosphate ◽  
Deficient Mice

scholarly journals The role of sphingolipids in endothelial barrier function

2015 ◽  
Vol 396 (6-7) ◽  
pp. 681-691 ◽  
Author(s):  
Peter L. Jernigan ◽  
Amy T. Makley ◽  
Richard S. Hoehn ◽  
Michael J. Edwards ◽  
Timothy A. Pritts
Keyword(s):  
Barrier Function ◽  
Biologically Active ◽  
Endothelial Barrier ◽  
Endothelial Barrier Function ◽  
Physiologic Function ◽  
Sphingosine 1 Phosphate ◽  
Endothelial Integrity ◽  
Pathologic Processes ◽  
Immense Potential

Abstract Sphingolipids are a ubiquitous family of essential lipids with an increasingly understood role as biologically active mediators in numerous physiologic and pathologic processes. Two particular sphingolipid species, sphingosine-1-phosphate and ceramide, and their metabolites interact both directly and indirectly with endothelial cells to regulate vascular permeability. Sphingosine-1-phosphate generally augments endothelial integrity while ceramide tends to promote vascular leak, and a tight balance between the two is necessary to maintain normal physiologic function. The mechanisms by which sphingolipids regulate endothelial barrier function are complex and occur through multiple different pathways, and disruptions or imbalances in these pathways have been implicated in a number of specific disease processes. With improved understanding of sphingolipid biology, endothelial function, and the interactions between the two, several targets for therapeutic intervention have emerged and there is immense potential for further advancement in this field.

scholarly journals High Density Lipoprotein-associated Sphingosine 1-Phosphate Promotes Endothelial Barrier Function

2008 ◽  
Vol 283 (36) ◽  
pp. 25074-25081 ◽  
Author(s):  
Kelley M. Argraves ◽  
Patrick J. Gazzolo ◽  
Eric M. Groh ◽  
Brent A. Wilkerson ◽  
Bryan S. Matsuura ◽  
...  
Keyword(s):  
High Density Lipoprotein ◽  
Barrier Function ◽  
Density Lipoprotein ◽  
High Density ◽  
Endothelial Barrier ◽  
Endothelial Barrier Function ◽  
Sphingosine 1 Phosphate
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