scholarly journals Effects of Regulator Binding on the Structure and Conformational Flexibility of the Amino Terminal Domains of Ionotropic Glutamate Receptors

2015 ◽  
Vol 29 (S1) ◽  
Author(s):  
Philip Varnes ◽  
Carlos Metz ◽  
Emily Bartle ◽  
J Ellis Bell
Steroids ◽  
2012 ◽  
Vol 77 (7) ◽  
pp. 774-779 ◽  
Author(s):  
Krasnodara Cameron ◽  
Emily Bartle ◽  
Ryan Roark ◽  
David Fanelli ◽  
Melissa Pham ◽  
...  

Author(s):  
Robin Herbrechter ◽  
Nadine Hube ◽  
Raoul Buchholz ◽  
Andreas Reiner

AbstractIonotropic glutamate receptors (iGluRs) play key roles for signaling in the central nervous system. Alternative splicing and RNA editing are well-known mechanisms to increase iGluR diversity and to provide context-dependent regulation. Earlier work on isoform identification has focused on the analysis of cloned transcripts, mostly from rodents. We here set out to obtain a systematic overview of iGluR splicing and editing in human brain based on RNA-Seq data. Using data from two large-scale transcriptome studies, we established a workflow for the de novo identification and quantification of alternative splice and editing events. We detected all canonical iGluR splice junctions, assessed the abundance of alternative events described in the literature, and identified new splice events in AMPA, kainate, delta, and NMDA receptor subunits. Notable events include an abundant transcript encoding the GluA4 amino-terminal domain, GluA4-ATD, a novel C-terminal GluD1 (delta receptor 1) isoform, GluD1-b, and potentially new GluK4 and GluN2C isoforms. C-terminal GluN1 splicing may be controlled by inclusion of a cassette exon, which shows preference for one of the two acceptor sites in the last exon. Moreover, we identified alternative untranslated regions (UTRs) and species-specific differences in splicing. In contrast, editing in exonic iGluR regions appears to be mostly limited to ten previously described sites, two of which result in silent amino acid changes. Coupling of proximal editing/editing and editing/splice events occurs to variable degree. Overall, this analysis provides the first inventory of alternative splicing and editing in human brain iGluRs and provides the impetus for further transcriptome-based and functional investigations.


2009 ◽  
Vol 392 (5) ◽  
pp. 1125-1132 ◽  
Author(s):  
Amber Clayton ◽  
Christian Siebold ◽  
Robert J.C. Gilbert ◽  
Geoffrey C. Sutton ◽  
Karl Harlos ◽  
...  

2021 ◽  
pp. 108631
Author(s):  
David Stroebel ◽  
Laetitia Mony ◽  
Pierre Paoletti

Sign in / Sign up

Export Citation Format

Share Document