Serum levels of interleukin-12 in adult and paediatric patients with HIV-1 infection

AIDS ◽  
1995 ◽  
Vol 9 (4) ◽  
pp. 405-406
Author(s):  
&NA;
Keyword(s):  
Serum Levels ◽  
Interleukin 12 ◽  
Paediatric Patients ◽  
Hiv 1

Lack of mother-to-child HIV-1 transmission is associated with elevated serum levels of 90 K immune modulatory protein

AIDS ◽  
2000 ◽  
Vol 14 (4) ◽  
pp. F41-F45 ◽  
Author(s):  
Piernicola Pelliccia ◽  
Luisa Galli ◽  
Maurizio de Martino ◽  
Francesco Chiarelli ◽  
Alberto Verrotti ◽  
...  
Keyword(s):  
Elevated Serum ◽  
Serum Levels ◽  
Mother To Child ◽  
Hiv 1

scholarly journals Intranasal Vaccination Using Interleukin-12 and Cholera Toxin Subunit B as Adjuvants To Enhance Mucosal and Systemic Immunity to Human Immunodeficiency Virus Type 1 Glycoproteins

2003 ◽  
Vol 77 (10) ◽  
pp. 5589-5597 ◽  
Author(s):  
Diana I. Albu ◽  
Agnes Jones-Trower ◽  
Amy M. Woron ◽  
Kathleen Stellrecht ◽  
Christopher C. Broder ◽  
...  
Keyword(s):  
Interleukin 12 ◽  
Mucosal Antibody ◽  
Immunodeficiency Virus ◽  
Cholera Toxin Subunit B ◽  
Iga Antibody ◽  
Antibody Levels ◽  
Subunit B ◽  
Anti Hiv ◽  
Hiv 1

ABSTRACT We have investigated the induction of protective mucosal immunity to human immunodeficiency virus type 1 (HIV-1) isolate 89.6 by intranasal (i.n.) immunization of mice with gp120 and gp140 together with interleukin-12 (IL-12) and cholera toxin subunit B (CTB) as adjuvants. It was found that both IL-12 and CTB were required to elicit mucosal antibody responses and that i.n. immunization resulted in increased total, immunoglobulin G1 (IgG1), and IgG2a anti-HIV-1 antibody levels in serum; increased total, IgG1, IgG2a, and IgA antibody expression in bronchoalveolar lavage fluids; and increased IgA antibody levels in vaginal washes. Levels of anti-HIV-1 antibodies in both sera and secretions were higher in groups immunized with gp140 than in those immunized with gp120. However, only gp120-specific mucosal antibodies demonstrated neutralizing activity against HIV-1 89.6. Taken together, the results show that IL-12 and CTB act synergistically to enhance both systemic and local mucosal antibody responses to HIV-1 glycoproteins and that even though gp140 induces higher antibody titers than gp120, only gp120-specific mucosal antibodies interfere with virus infectivity.

scholarly journals The Safety and Immunogenicity of an Interleukin-12–Enhanced Multiantigen DNA Vaccine Delivered by Electroporation for the Treatment of HIV-1 Infection

2016 ◽  
Vol 71 (2) ◽  
pp. 163-171 ◽  
Author(s):  
Jeffrey M. Jacobson ◽  
Lu Zheng ◽  
Cara C. Wilson ◽  
Pablo Tebas ◽  
Roy M. Matining ◽  
...  
Keyword(s):  
Dna Vaccine ◽  
Interleukin 12 ◽  
Hiv 1

Assessment of etravirine resistance in HIV-1-infected paediatric patients using population and deep sequencing: final results of the PIANO study

2015 ◽  
Vol 21 (4) ◽  
pp. 317-327 ◽  
Author(s):  
Lotke Tambuyzer ◽  
Kim Thys ◽  
Annemie Hoogstoel ◽  
Steven Nijs ◽  
Frank Tomaka ◽  
...  
Keyword(s):  
Deep Sequencing ◽  
Paediatric Patients ◽  
Piano Study ◽  
Hiv 1

scholarly journals Immunological and pharmacological strategies to reactivate HIV-1 from latently infected cells: a possibility for HIV-1 paediatric patients?

2015 ◽  
Vol 1 (3) ◽  
pp. 148-152 ◽  
Author(s):  
M. Martínez-Bonet ◽  
M.I. Clemente ◽  
M.J. Serramía ◽  
S. Moreno ◽  
E. Muñoz ◽  
...  
Keyword(s):  
Paediatric Patients ◽  
Latently Infected ◽  
Infected Cells ◽  
Hiv 1

scholarly journals Sustained expansion of NKT cells and antigen-specific T cells after injection of α-galactosyl-ceramide loaded mature dendritic cells in cancer patients

2005 ◽  
Vol 201 (9) ◽  
pp. 1503-1517 ◽  
Author(s):  
David H. Chang ◽  
Keren Osman ◽  
John Connolly ◽  
Anjli Kukreja ◽  
Joseph Krasovsky ◽  
...  
Keyword(s):  
T Cells ◽  
Dendritic Cells ◽  
Advanced Cancer ◽  
Serum Levels ◽  
Nkt Cells ◽  
Interleukin 12 ◽  
Cell Immunity ◽  
Galactosyl Ceramide ◽  
Inducible Protein

Natural killer T (NKT) cells are distinct glycolipid reactive innate lymphocytes that are implicated in the resistance to pathogens and tumors. Earlier attempts to mobilize NKT cells, specifically, in vivo in humans met with limited success. Here, we evaluated intravenous injection of monocyte-derived mature DCs that were loaded with a synthetic NKT cell ligand, α-galactosyl-ceramide (α-GalCer; KRN-7000) in five patients who had advanced cancer. Injection of α-GalCer–pulsed, but not unpulsed, dendritic cells (DCs) led to >100-fold expansion of several subsets of NKT cells in all patients; these could be detected for up to 6 mo after vaccination. NKT activation was associated with an increase in serum levels of interleukin-12 p40 and IFN-γ inducible protein-10. In addition, there was an increase in memory CD8+ T cells specific for cytomegalovirus in vivo in response to α-GalCer–loaded DCs, but not unpulsed DCs. These data demonstrate the feasibility of sustained expansion of NKT cells in vivo in humans, including patients who have advanced cancer, and suggest that NKT activation might help to boost adaptive T cell immunity in vivo.

scholarly journals Human Immunodeficiency Virus Type 1 gp120 Induces Abnormal Maturation and Functional Alterations of Dendritic Cells: a Novel Mechanism for AIDS Pathogenesis

2004 ◽  
Vol 78 (18) ◽  
pp. 9763-9772 ◽  
Author(s):  
Laura Fantuzzi ◽  
Cristina Purificato ◽  
Karim Donato ◽  
Filippo Belardelli ◽  
Sandra Gessani
Keyword(s):  
Human Immunodeficiency Virus ◽  
Dendritic Cells ◽  
Human Immunodeficiency Virus Type ◽  
Virus Type ◽  
Interleukin 12 ◽  
Activation Markers ◽  
Antigen Uptake ◽  
Immunodeficiency Virus ◽  
Hiv 1

ABSTRACT Dendritic cells (DCs) play a crucial role in bridging innate and acquired immune responses to pathogens. In human immunodeficiency virus type 1 (HIV-1) infection, immature DCs (iDCs) are also main targets for HIV-1 at the mucosal level. In this study, we evaluated the effects of HIV-1-DC interactions on the maturation and functional activity of these cells. Exposure of human monocyte-derived iDCs to either aldrithiol-2-inactivated HIV-1 or gp120 led to an upmodulation of activation markers indicative of functional maturation. Despite their phenotype, these cells retained antigen uptake capacity and showed an impaired ability to secrete cytokines or chemokines and to induce T-cell proliferation. Although gp120 did not interfere with DC differentiation, the capacity of these cells to produce interleukin-12 (IL-12) upon maturation was markedly reduced. Likewise, iDCs stimulated by classical maturation factors in the presence of gp120 lacked allostimulatory capacity and did not produce IL-12, in spite of their phenotype typical of activated DCs. Exogenous addition of IL-12 restores the allostimulatory capacity of gp120-exposed DCs. The finding that gp120 induces abnormal maturation of DCs linked to profound suppression of their activities unravels a novel mechanism by which HIV can lead to immune dysfunction in AIDS patients.

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