scholarly journals Endothelial Cell Seeding onto the Extracellular Matrix of Fibroblasts for the Development of a Small Diameter Polyurethane Vessel

ASAIO Journal ◽  
1993 ◽  
Vol 39 (3) ◽  
pp. M740-M745 ◽  
Author(s):  
Yoon-Shin Lee ◽  
Dong Kook Park ◽  
Yong Bae Kim ◽  
Jeong Wook Seo ◽  
Kyu Back Lee ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Endothelial Cell ◽  
Small Diameter ◽  
Cell Seeding ◽  
Endothelial Cell Seeding

Endothelial Cell Seeding onto the Extracellular Matrix of Fibroblasts for the Development of a Small Diameter Polyurethane Vessel

ASAIO Journal ◽  
1993 ◽  
Vol 39 (3) ◽  
pp. M740-M745 ◽  
Author(s):  
YOON-SHIN LEE ◽  
DONG KOOK PARK ◽  
YONG BAE KIM ◽  
JEONG WOOK SEO ◽  
KYU BACK LEE ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Endothelial Cell ◽  
Small Diameter ◽  
Cell Seeding ◽  
Endothelial Cell Seeding

Microvascular Endothelial Cell Seeding of Small-Diameter Dacron|2TM|0 Vascular Grafts

1988 ◽  
Vol 1 (1) ◽  
pp. 35-44 ◽  
Author(s):  
Steven P. Schmidt ◽  
Navid Monajjem ◽  
M. Michelle Evancho ◽  
Todd R. Pippert ◽  
W. V. Sharp
Keyword(s):  
Endothelial Cell ◽  
Vascular Grafts ◽  
Small Diameter ◽  
Microvascular Endothelial Cell ◽  
Cell Seeding ◽  
Endothelial Cell Seeding ◽  
Microvascular Endothelial

Influence of endothelial cell seeding on platelet deposition and patency in small-diameter Dacron arterial grafts

1984 ◽  
Vol 1 (1) ◽  
pp. 224-233 ◽  
Author(s):  
Brent T. Allen ◽  
Julie A. Long ◽  
Richard E. Clark ◽  
Gregorio A. Sicard ◽  
Kevin T. Hopkins ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Small Diameter ◽  
Cell Seeding ◽  
Arterial Grafts ◽  
Platelet Deposition ◽  
Endothelial Cell Seeding

Endothelial Cell Seeding on Crosslinked Collagen: Effects of Crosslinking on Endothelial Cell Proliferation and Functional Parameters

2000 ◽  
Vol 84 (08) ◽  
pp. 325-331 ◽  
Author(s):  
M. J. B. Wissink ◽  
M. J. A. van Luyn ◽  
R. Beernink ◽  
F. Dijk ◽  
A. A. Poot ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Endothelial Cell ◽  
Plasminogen Activator ◽  
Type I Collagen ◽  
Vascular Grafts ◽  
Small Diameter ◽  
Endothelial Cell Proliferation ◽  
Type I ◽  
Cell Seeding ◽  
Endothelial Cell Seeding

SummaryEndothelial cell seeding, a promising method to improve the performance of small-diameter vascular grafts, requires a suitable substrate, such as crosslinked collagen. Commonly used crosslinking agents such as glutaraldehyde and formaldehyde cause, however, cytotoxic reactions and thereby hamper endothelialization of currently available collagen-coated vascular graft materials.The aim of this study was to investigate the effects of an alternative method for crosslinking of collagen, using N-(3-dimethylaminopropyl)-N’-ethylcarbodiimide (EDC) in combination with N-hydroxysuccinimide (NHS), on various cellular functions of human umbilical vein endothelial cells (HUVECs) in vitro. Compared to non-crosslinked type I collagen, proliferation of seeded endothelial cells was significantly increased on EDC/NHS-crosslinked collagen. Furthermore, higher cell numbers were found with increasing crosslink densities. Neither the morphology of the cells nor the secretion of prostacyclin (PGI2), von Willebrand factor (vWF), tissue plasminogen activator (t-PA) and plasminogen activator inhibitor (PAI-1) was affected by the crosslink density of the collagen substrate. Therefore, EDC/NHScrosslinked collagen is candidate substrate for in vivo application such as endothelial cell seeding of collagen-coated vascular grafts.

scholarly journals Endothelial Cell Seeding of Small Diameter Vascular Grafts

1990 ◽  
Vol 14 (5) ◽  
pp. 355-360 ◽  
Author(s):  
N. L. James ◽  
K. Schindhelm ◽  
P. Slowiaczek ◽  
B. K. Milthorpe ◽  
N. P. B. Dudman ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Vascular Grafts ◽  
Small Diameter ◽  
Cell Seeding ◽  
Endothelial Cell Seeding ◽  
Small Diameter Vascular Grafts

scholarly journals Influence of endothelial cell seeding on platelet deposition and patency in small-diameter Dacron arterial grafts

1984 ◽  
Vol 1 (1) ◽  
pp. 224-233 ◽  
Author(s):  
Brent T. Allen ◽  
Julie A. Long ◽  
Richard E. Clark ◽  
Gregorio A. Sicard ◽  
Kevin T. Hopkins ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Small Diameter ◽  
Cell Seeding ◽  
Arterial Grafts ◽  
Platelet Deposition ◽  
Endothelial Cell Seeding
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