Electrostatic endothelial cell seeding technique for small diameter (<6 mm) vascular prostheses: Feasibility testing

Multiple studies have indicated the importance of surface charge in the adhesion of multiple cardiovascular cell lines including platelets and endothelial cells on the substrate materials (1,4,7-10,12-15). It is the purpose of this article to report a feasibility study conducted using an electrostatic endothelial cell seeding technique. The feasibility study was conducted using human umbilical vein endothelial cells (HUVEC), a static pool apparatus, a voltage source, and a parallel plate capacitor. The HUVEC concentration and seeding times were constant at 560,000 HUVEC/ml and 30 min, respectively. Scanning electron microscopy examination of the endothelial cell adhesion indicated that an induced temporary positive surface charge on e-PTFE graft material enhances the number and the maturation (flattening) of HUVECs adhered. The results indicated that the total number of endothelial cells adhered (70.9 mm2) was increased from 9198 ± 1194 HUVECs on the control (no induced surface charge) e-PTFE to 22,482 ± 4814 HUVECs (2.4 × control) on the maximum induced positive surface charge. The total number of cells in the flattened phase of adhesion increased from 837 ± 275 to 6785 ± 1012 HUVECs (8.1 ×) under identical conditions. Thus, the results of the feasibility study support the premise that electrostatic interaction is an important factor in both the endothelial cell adhesion and spreading processes and suggest that the electrostatic seeding technique may lead to an increased patency of small diameter (<6 mm) vascular prostheses.

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Endothelial Cell Seeding onto the Extracellular Matrix of Fibroblasts for the Development of a Small Diameter Polyurethane Vessel

ASAIO Journal ◽

10.1097/00002480-199339030-00113 ◽

1993 ◽

Vol 39 (3) ◽

pp. M740-M745 ◽

Cited By ~ 11

Author(s):

Yoon-Shin Lee ◽

Dong Kook Park ◽

Yong Bae Kim ◽

Jeong Wook Seo ◽

Kyu Back Lee ◽

...

Keyword(s):

Extracellular Matrix ◽

Endothelial Cell ◽

Small Diameter ◽

Cell Seeding ◽

Endothelial Cell Seeding

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Endothelial Cell Seeding of Polytetrafluoroethylene Vascular Prostheses Coated With a Fibroblastic Matrix

Annals of Vascular Surgery ◽

10.1007/bf02000149 ◽

1993 ◽

Vol 7 (6) ◽

pp. 549-555 ◽

Cited By ~ 13

Author(s):

J.M. Bellón ◽

J. Buján ◽

N.G. Honduvilla ◽

A. Hernando ◽

J. Navlet

Keyword(s):

Endothelial Cell ◽

Cell Seeding ◽

Vascular Prostheses ◽

Endothelial Cell Seeding

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Microvascular Endothelial Cell Seeding of Small-Diameter Dacron|2TM|0 Vascular Grafts

Journal of Investigative Surgery ◽

10.3109/08941938809141074 ◽

1988 ◽

Vol 1 (1) ◽

pp. 35-44 ◽

Cited By ~ 13

Author(s):

Steven P. Schmidt ◽

Navid Monajjem ◽

M. Michelle Evancho ◽

Todd R. Pippert ◽

W. V. Sharp

Keyword(s):

Endothelial Cell ◽

Vascular Grafts ◽

Small Diameter ◽

Microvascular Endothelial Cell ◽

Cell Seeding ◽

Endothelial Cell Seeding ◽

Microvascular Endothelial

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Influence of endothelial cell seeding on platelet deposition and patency in small-diameter Dacron arterial grafts

Journal of Vascular Surgery ◽

10.1067/mva.1984.avs0010224 ◽

1984 ◽

Vol 1 (1) ◽

pp. 224-233 ◽

Cited By ~ 7

Author(s):

Brent T. Allen ◽

Julie A. Long ◽

Richard E. Clark ◽

Gregorio A. Sicard ◽

Kevin T. Hopkins ◽

...

Keyword(s):

Endothelial Cell ◽

Small Diameter ◽

Cell Seeding ◽

Arterial Grafts ◽

Platelet Deposition ◽

Endothelial Cell Seeding

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Endothelial Cell Seeding on Crosslinked Collagen: Effects of Crosslinking on Endothelial Cell Proliferation and Functional Parameters

Thrombosis and Haemostasis ◽

10.1055/s-0037-1614015 ◽

2000 ◽

Vol 84 (08) ◽

pp. 325-331 ◽

Cited By ~ 51

Author(s):

M. J. B. Wissink ◽

M. J. A. van Luyn ◽

R. Beernink ◽

F. Dijk ◽

A. A. Poot ◽

...

Keyword(s):

Endothelial Cells ◽

Endothelial Cell ◽

Plasminogen Activator ◽

Type I Collagen ◽

Vascular Grafts ◽

Small Diameter ◽

Endothelial Cell Proliferation ◽

Type I ◽

Cell Seeding ◽

Endothelial Cell Seeding

SummaryEndothelial cell seeding, a promising method to improve the performance of small-diameter vascular grafts, requires a suitable substrate, such as crosslinked collagen. Commonly used crosslinking agents such as glutaraldehyde and formaldehyde cause, however, cytotoxic reactions and thereby hamper endothelialization of currently available collagen-coated vascular graft materials.The aim of this study was to investigate the effects of an alternative method for crosslinking of collagen, using N-(3-dimethylaminopropyl)-N’-ethylcarbodiimide (EDC) in combination with N-hydroxysuccinimide (NHS), on various cellular functions of human umbilical vein endothelial cells (HUVECs) in vitro. Compared to non-crosslinked type I collagen, proliferation of seeded endothelial cells was significantly increased on EDC/NHS-crosslinked collagen. Furthermore, higher cell numbers were found with increasing crosslink densities. Neither the morphology of the cells nor the secretion of prostacyclin (PGI2), von Willebrand factor (vWF), tissue plasminogen activator (t-PA) and plasminogen activator inhibitor (PAI-1) was affected by the crosslink density of the collagen substrate. Therefore, EDC/NHScrosslinked collagen is candidate substrate for in vivo application such as endothelial cell seeding of collagen-coated vascular grafts.

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