Detection of Human Papillomavirus (HPV) DNA in Cervical Swabs by the Polymerase Chain Reaction

1994 ◽  
Vol 13 (2) ◽  
pp. 139-142 ◽  
Author(s):  
Ulla Hørding ◽  
Søren Daugaard ◽  
Johannes E. Bock
Keyword(s):  
Polymerase Chain Reaction ◽  
Human Papillomavirus ◽  
Chain Reaction ◽  
Hpv Dna ◽  
Polymerase Chain

Polymerase chain reaction (PCR) identification of human papillomavirus (HPV) DNA in verrucous carcinoma of the larynx

1993 ◽  
Vol 103 (4) ◽  
pp. 416-420 ◽  
Author(s):  
Jan L. Kasperbauer ◽  
Gerard L. O'Halloran ◽  
Mark J. Espy ◽  
Thomas F. Smith ◽  
Jean E. Lewis
Keyword(s):  
Polymerase Chain Reaction ◽  
Human Papillomavirus ◽  
Verrucous Carcinoma ◽  
Chain Reaction ◽  
Hpv Dna ◽  
Pcr Identification ◽  
Carcinoma Of The Larynx ◽  
Polymerase Chain

Human papillomavirus genotyping by a polymerase chain reaction-based genechip method in cervical carcinoma treated with neoadjuvant chemotherapy plus radical surgery

2004 ◽  
Vol 14 (4) ◽  
pp. 639-649 ◽  
Author(s):  
H. J. Huang ◽  
S. L. Huang ◽  
C. Y. Lin ◽  
R. W. Lin ◽  
F. Y. Chao ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Human Papillomavirus ◽  
Cervical Carcinoma ◽  
Radical Surgery ◽  
Chain Reaction ◽  
Hpv 16 ◽  
Hpv Dna ◽  
Hpv Genotyping ◽  
Polymerase Chain ◽  
Hpv 18

The aim of this study was to evaluate the accuracy of human papillomavirus (HPV) genotyping by a polymerase chain reaction (PCR)-based genechip method and to determine the prognostic value of HPV genotype in bulky stage IB or IIA cervical carcinoma treated with neoadjuvant chemotherapy (NAC) and radical surgery. A total of 149 patients had adequate tissue for the study. The SPF1/GP6+ primers were used to amplify a 184 bp fragment. The amplimers were submitted for direct sequencing and hybridization with a genechip using revert-blot detection of 39 types of HPV DNA in a single reaction. Two runs of PCR with respective hybridization were performed for each tumor. The complete concordance of HPV genotyping was 80.5% (120/149) of the paired genechip results. The kappa coefficient was 0.634 (P < 0.0001). HPV DNA sequences were detected in 100% of the specimens, among which 67.8% harbored single type and 32.2% contained multiple types. HPV-16 was detected in 98.7%, HPV-18 in 22.8%, HPV-31 in 0.7%, HPV-45 in 1.3%, HPV-52 in 2.0%, HPV-58 in 6.7%, HPV-59 in 4.7%, and HPV-67 in 0.7%. In multivariate analyses, the HPV genotype [HPV-18 or HPV-16 and HPV-18 only versus all others: relative risk (RR), 2.33; 95% CI, 1.17–4.64; P = 0.016] and pre-NAC tumor size (>5 versus ≤5 cm: RR, 2.25; 95% CI, 1.13–4.48; P = 0.021) were significantly related to overall survival. This PCR-based genechip method is sensitive and reproducible for HPV genotyping. The association of HPV-18 or HPV-16 and HPV-18 with poor outcome in cervical carcinoma treated with NAC plus radical surgery is confirmed.

Evaluation of a New Multiplex Real-Time Polymerase Chain Reaction Assay for the Detection of Human Papillomavirus Infections in a Referral Population

2012 ◽  
Vol 22 (6) ◽  
pp. 1050-1056 ◽  
Author(s):  
Matthias Jentschke ◽  
Philipp Soergel ◽  
Victoria Lange ◽  
Boštjan Kocjan ◽  
Thilo Doerk ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Human Papillomavirus ◽  
High Risk ◽  
Polymerase Chain Reaction Assay ◽  
Clinical Performance ◽  
Chain Reaction ◽  
Hpv Dna ◽  
Hpv Genotypes ◽  
Polymerase Chain ◽  
High Risk Hpv

ObjectivesHuman papillomavirus (HPV) testing is an important part of cervical cancer screening and management of women with atypical screening results. This study was conducted to evaluate the analytical and clinical performance of the Abbott RealTime High-Risk HPV assay (RealTime) in a referral population, in comparison to the Qiagen Hybrid Capture 2 High-Risk HPV DNA Test (hc2).MethodsRealTime is a new polymerase chain reaction assay that detects 14 high-risk HPV genotypes with simultaneous differentiation between HPV 16 and HPV 18. Five hundred forty-five routine cervical smear samples (ThinPrep) from women who were referred to 2 German colposcopy clinics were included in the study. All samples were tested with both assays for the detection of high-risk HPV DNA. Specimens with repeatedly discordant results were genotyped by Linear Array (Roche) and in-house polymerase chain reaction assays.ResultsBoth assays showed excellent overall agreement (92.8%; κ = 0.86) on 545 samples. Analytical sensitivity of RealTime was comparable to that of hc2 (97.6% vs 95.1%,P= 0.189), whereas RealTime demonstrated significantly higher analytical specificity compared with hc2 (100% vs 93.1%,P< 0.0001). RealTime showed no cross-reactivity with untargeted HPV genotypes in this study. The clinical performance of the assays was evaluated based on histology results available from 319 women (90 nonpathological, 73 cervical intraepithelial neoplasia [CIN] 1, 75 CIN 2, 74 CIN 3, and 7 invasive cancers). High-risk HPV detection rates observed in women with CIN 1, CIN 2+, and CIN 3+ diagnosis, respectively, were comparable for both assays: 47.9%, 92.3%, and 97.5% (RealTime) and 47.9%, 92.3%, and 93.8% (hc2). Detection of HPV 16/18 with RealTime was highly correlated with severity of dysplasia: less than CIN 2, 30.5%; CIN 2+, 59.0%; CIN 3+, 71.6%.ConclusionsThese results support the use of RealTime for routine detection of HPV infections in a referral population.

Detection of human papillomavirus (HPV) DNA in human prostatic tissues by polymerase chain reaction (PCR)

The Prostate ◽  
1993 ◽  
Vol 22 (2) ◽  
pp. 171-180 ◽  
Author(s):  
Fazlul H. Sarkar ◽  
Wael A. Sakr ◽  
Yi-Wei Li ◽  
Prabhakar Sreepathi ◽  
John D. Crissman
Keyword(s):  
Polymerase Chain Reaction ◽  
Human Papillomavirus ◽  
Chain Reaction ◽  
Hpv Dna ◽  
Polymerase Chain

Analysis of Prevalence of Human Papillomavirus in Laryngeal Carcinomas Study of 40 Cases Using Polymerase Chain Reaction and Consensus Primers

1993 ◽  
Vol 102 (4) ◽  
pp. 309-313 ◽  
Author(s):  
Margaret S. Brandwein ◽  
Gerard J. Uuovo ◽  
Hugh Biller
Keyword(s):  
Polymerase Chain Reaction ◽  
Human Papillomavirus ◽  
Chain Reaction ◽  
Hpv Dna ◽  
Hot Start ◽  
Laryngeal Carcinomas ◽  
Polymerase Chain ◽  
Consensus Primers ◽  
Disease Free

In this study we determined the frequency of association of human papillomavirus (HPV) and laryngeal carcinomas and investigated the possibility that HPV may be associated with larger or more aggressive tumors. Laryngeal squamous cell carcinomas from 40 patients who did not have preexisting papillomas by clinical history were retrieved from formalin-fixed, paraffin-embedded blocks and analyzed for HPV. Twenty-two cases were tumors of the true vocal folds, and 18 were supraglottic. Clinical follow-up was available for 25 patients. We used the polymerase chain reaction (PCR) with the “hot start” modification and consensus primers that can detect over 30 distinct HPV types. Three of the 40 patients (8%) had detectable HPV DNA. These 3 patients did not have unusual age demographics and were smokers. All but 1 of the 22 HPV-negative patients who were questioned were also smokers. We compared the outcomes for large (4 cm or greater) HPV-positive and -negative tumors. Six of the 40 tumors were 4 cm or greater and involved contiguous structures. Two of these 6 were HPV-positive, and these patients died of disease after 3 and 16 months, respectively. Of the 4 HPV-negative patients with tumors greater than 4 cm, 3 are disease-free at 41, 42, and 3 months, respectively, and 1 was lost to follow-up. The third HPV-positive patient had a tumor less than 1 cm, and is disease-free after 38 months. While the number of HPV-positive cases is too small for definitive conclusions, it is possible that for large tumors the presence of HPV DNA may portend a worse prognosis. However, in the population of patients with laryngeal carcinoma whom we have studied, we conclude that the L1 region of HPV DNA is rarely seen to be present in the tumors by “hot start” PCR, and hence HPV is probably not a common cancer promoter for laryngeal carcinoma.

Detection of Human Papillomavirus on Papanicolaou-Stained Cervical Smears Using Indirect In Situ Polymerase Chain Reaction Hybridization

2001 ◽  
Vol 125 (3) ◽  
pp. 353-357
Author(s):  
Mahmood Manavi ◽  
Margit Bauer ◽  
Kerstin Pischinger ◽  
Klaus Czerwenka
Keyword(s):  
Polymerase Chain Reaction ◽  
Human Papillomavirus ◽  
Squamous Intraepithelial Lesions ◽  
Chain Reaction ◽  
Hpv Dna ◽  
Cervical Smears ◽  
Polymerase Chain ◽  
Dna Types ◽  
Intraepithelial Lesions

Abstract Objective.—Polymerase chain reaction (PCR) and indirect in situ hybridization were combined to detect human papillomavirus (HPV) DNA on Papanicolaou (PAP)-stained cervical smears. To our knowledge, this is the first report of an experiment using indirect in situ PCR (IS-PCR) on PAP-stained cervical smears. Design.—We collected native cell specimens from cervicovaginal lavage of 162 patients with squamous intraepithelial lesions. Solution-phase PCR (SP-PCR) was performed as the reference method in the detection of HPV DNA. Indirect IS-PCR was carried out for the same patients to detect the HPV DNA types 6/11 and 16/18 after the PAP-stained smears had been decolorized. Low-risk and high-risk HPV DNA types were also detected by both SP-PCR and indirect IS-PCR. Results.—In the evaluation by indirect IS-PCR, 48 of 81 PAP-stained cell smears of low-grade squamous intraepithelial lesions were positive for HPV DNA, as compared to 40 positive cell smears determined by indirect SP-PCR (sensitivity of indirect IS-PCR compared to SP-PCR, 98.1%). Forty-two of 42 high-grade squamous intraepithelial lesion samples were positive for HPV DNA, as determined by both methods (sensitivity of IS-PCR, 100%). Cell lines investigated in this study as positive or negative controls for HPV DNA were confirmed by indirect IS-PCR and SP-PCR. Conclusions.—Our data show that in comparison to SP-PCR, indirect IS-PCR is a highly sensitive method to detect HPV DNA in cell smears from the uterine cervix. The advantages of indirect IS-PCR are (a) low numbers of cells needed, (b) the possibility of using PAP-stained specimens, and (c) cytologic details of smears can be preserved.

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