Basic Fibroblast Growth Factor Increases Constitutive Nitric Oxide Synthase During Healing of Rat Gastric Ulcers

1997 ◽  
Vol 25 ◽  
pp. S122-S128 ◽  
Author(s):  
Yasutada Akiba ◽  
Masahiko Nakamura ◽  
Masaya Oda ◽  
Hiroyuki Kimura ◽  
Soichiro Miura ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Growth Factor ◽  
Nitric Oxide Synthase ◽  
Fibroblast Growth Factor ◽  
Basic Fibroblast Growth Factor ◽  
Gastric Ulcers ◽  
Fibroblast Growth ◽  
Constitutive Nitric Oxide Synthase ◽  
Oxide Synthase

Basic fibroblast growth factor increases nitric oxide synthase production in bovine endothelial cells

1995 ◽  
Vol 269 (5) ◽  
pp. H1583-H1589 ◽  
Author(s):  
S. K. Kostyk ◽  
S. Kourembanas ◽  
E. L. Wheeler ◽  
D. Medeiros ◽  
L. P. McQuillan ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Endothelial Cells ◽  
Growth Factor ◽  
Nitric Oxide Synthase ◽  
Fibroblast Growth Factor ◽  
Basic Fibroblast Growth Factor ◽  
Blot Analysis ◽  
Fibroblast Growth ◽  
Oxide Synthase

Basic fibroblast growth factor (bFGF) and nitric oxide (NO) are expressed by endothelial cells (EC) and are involved in regulation of endothelial functions. In vivo, bFGF has a hypotensive effect which is mediated, in part, through activation of nitric oxide synthase (NOS) and the subsequent generation of NO. Thus we hypothesized that regulation of NOS in EC might be modulated by bFGF. bFGF treatment of EC in vitro resulted in increased NADPH diaphorase staining, a histochemical marker associated with the presence of NOS. Using cGMP generation in a reporter cell as a bioassay for NO release, we demonstrated that bFGF treatment of EC leads to increased production of biologically active NO. Furthermore, bFGF treatment of EC resulted in an increase in cellular content of the endothelial form of NOS as shown by Western blot analysis. Finally, Northern blot analysis was used to demonstrate that message levels of the constitutive, calcium-dependent, endothelial form of NOS is increased in EC by treatment with bFGF in vitro. These results suggest that bFGF has potential to regulate vascular tone through the modulation of levels of endothelial NOS.

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